FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089802
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC48560.4455855283803847No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45260.41530479848633056No Hit
CCCATATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC39190.3596066074387825No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACA37620.3452003207922173No Hit
GATTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT37000.3395112139636374No Hit
GTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA35960.32996819605763245No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG35760.3281330003064777No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACACAGTAATAAT34060.31253383642166194No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32740.30042154446404024No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32480.29803578998753905No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG29810.27353592670962246No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29110.2671127415805807No Hit
GTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG27500.25233941578378455No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT27410.25151357769576493No Hit
ATGTATTACTGTGCGAGATGTTATAGGGGCTACGGTGACTACGTTGACTA26730.2452739121418386No Hit
GTCTCTGGTGGCTCCATCAGCGATAGTAGTTACTACTGGGGCTGGATCCG26180.24022712382616293No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25270.2318769831584086No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24960.22903242974411867No Hit
CTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAA24760.22719723399296385No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCAT24240.22242572503996136No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG23560.2161860594860351No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23460.21526846161045768No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22890.2100381537196665No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22410.205633683916895No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG22380.20535840455422177No Hit
CTACTAGAGACTGAGCCAGAGGTCAAGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGG22270.20434904689108666No Hit
ATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA21810.2001280966634306No Hit
GTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC21270.19517306813531266No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20930.1920532353583495No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20520.18829108406848216No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT20020.18370309469059518No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG19870.18232669787722908No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG19390.17792222807445757No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18940.17379303763435927No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18760.17214136145831993No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18730.17186608209564674No Hit
CAGTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA18560.17030616570716514No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18250.1674616122928752No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17400.15966203035046733No Hit
CCCCTGGAGGGACGGTCACACTCACTTGTGGCTTGACCTCTGGCTCAGTC17380.15947851077535186No Hit
GATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACGGTCACA16950.15553283991036904No Hit
CTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC16950.15553283991036904No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16880.15489052139746487No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT16850.15461524203479166No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16750.15369764415921425No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16510.15149540925782848No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16470.15112837010759753No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16430.15076133095736657No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16310.1496602135066737No Hit
GTATTACTGTGCGAGATGTTATAGGGGCTACGGTGACTACGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT16220.14883437541865402No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCGATAGTAGTTACTACTGGG15880.14571454264169087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15800.14498046434122897No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACGGTCACACTCACTTGTGGCTTGACCTCTGG15760.144613425190998No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15690.14397110667809382No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC15510.1423194305020545No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15200.13947487708776457No Hit
ACTAGAGACTGAGCCAGAGGTCAAGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGGCTC15000.13763968133660978No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14890.13663032367347464No Hit
CACTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG14770.13552920622278175No Hit
GGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC14520.13323521153383824No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTAC14470.13277641259604955No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14440.13250113323337634No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14270.13094121684489476No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14180.13011537875687512No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTACTAGAGACTGAG13940.12791314385548935No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13800.126628506829681No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13630.12506859044119942No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13430.12323339469004463No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13400.1229581153273714No Hit
CAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC13380.12277459575225592No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13180.12093940000110111No Hit
CTCCATATCCGTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCT13160.12075588042598565No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12940.11873716509971535No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT12920.11855364552459988No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12920.11855364552459988No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12800.117452528073907No Hit
CTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAG12650.1160761312605409No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12540.11506677359740577No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12400.1137821365715974No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12340.11323157784625099No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG12250.11240573975823132No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC12240.11231397997067358No Hit
GTCTATTATAGTGGGAGCACCCACTACAACCCGTCCCTCAAGAGTCGAGT12240.11231397997067358No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGAT12230.11222222018311583No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12210.11203870060800035No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA11980.10992822549417233No Hit
CTATTATAGTGGGAGCACCCACTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACACGTCCAAGAA11900.10919414719371041No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11880.10901062761859492No Hit
ACCCATATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT11710.10745071123011336No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAA11660.10699191229232466No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC11600.10644135356697822No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11420.1047896773909389No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11410.10469791760338117No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCA11300.10368855994024603No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11200.10277096206466863No Hit
GCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACACAGTAATAATCAGATTCATCATCTG11080.10166984461397574No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG11050.10139456525130253No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTT11000.10093576631351384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)