FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045332
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTATACGGCCGTGTCGTCGGCTCTGAGACTGTTCATTTGCAGATACA45640.4366076997547191No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC45570.4359380560434388No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGGGACCC37880.36237291118993775No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGATGTCACTCCACG36160.3459188085699089No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29590.2830679630968917No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCAGCGTGGAGTGACATCCTGAATGGCTGGGTGTTCGGCGGAGGGAC28850.27598887243478626No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGG28530.2729276440403623No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT28440.27206667355443054No Hit
TCCTCAGACTGCACCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC28060.26843146483605207No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27810.26603988015290836No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26670.25513425399777295No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25470.24365464751868307No Hit
GTATACTACTGTGCGAAAGGGCAGGTCTGGCGCGATTACTATTACTATTT25410.24308066719472857No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGGTCCCTTCA25200.24107173606088783No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24610.23542759620866863No Hit
GATTATTATTGTGCAGCGTGGAGTGACATCCTGAATGGCTGGGTGTTCGG23600.22576559408876798No Hit
GTCTTCTCCACGGTGGTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22820.21830384987735954No Hit
GTGCTGACTCAGCCACCCTCAACGTCTGGGACCCCCGGGCAGAGGGTCAC22630.2164862455181703No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC22270.21304236357444334No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22180.2121813930885116No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20750.19850152870092946No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20480.19591861724313425No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGGTGCAGT20310.19429233965859652No Hit
AGGTAATACTATAAATTGGTATCAGCAGGTCCCAGGAACGGCCCCCAAAC20010.19142243803882403No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20000.19132677465149828No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC19810.18950917029230904No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19800.1894135069049833No Hit
ATAATAATCAGCCTCATCCTCAGACTGCACCCCACTGATGGCCAGGGAGG19740.18883952658102882No Hit
CCCCTGAGGGCCGCCAATTATTACTATAGATGAGGAGTTTGGGGGCCGTT19240.18405635721474134No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19120.18290839656683236No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18590.17783823703856766No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17670.16903720540459874No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17660.168941542017273No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17140.163967045876334No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17110.16368005571435676No Hit
GCCGTATACTACTGTGCGAAAGGGCAGGTCTGGCGCGATTACTATTACTA16740.16014051038330407No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16620.15899254973539506No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16330.15621831150294835No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGAAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG16330.15621831150294835No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15420.14751294325630518No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT15420.14751294325630518No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15360.14693896293235068No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15330.14665197277037345No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14860.14215579356606323No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14460.13832925807303326No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14460.13832925807303326No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14080.1346940493546548No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGA14040.13431139580535179No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13970.13364175209407153No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13860.1325894548334883No Hit
GCACAATAATAATCAGCCTCATCCTCAGACTGCACCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG13740.13144149418557932No Hit
CCCTCAACGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG13650.13058052369964757No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13640.13048486031232182No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG13590.13000654337569306No Hit
GCCACATACCGCTCATCCGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCAAGAACACG13450.1286672559531326No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13220.12646699804464037No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13050.12484072046010262No Hit
CTCTTGTTCTGGAAGCAACTCCAACATCCTAGGTAATACTATAAATTGGTATCAGCAGGTCCCAGGAACGGCCCC13050.12484072046010262No Hit
GTATACGGCCGTGTCGTCGGCTCTGAGACTGTTCATTTGCAGATACAGCG12960.12397974997417088No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12900.1234057696502164No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12860.1230231161009134No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12720.12168382867835291No Hit
GTATTACCTAGGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGACCCT12480.11938790738253492No Hit
TTATTGTGCAGCGTGGAGTGACATCCTGAATGGCTGGGTGTTCGGCGGAG12390.11852693689660318No Hit
GTATTAGTGGGAGTGGTGGTGCCACATACCGCTCATCCGCAGACTCCGTG12350.1181442833473002No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT12260.11728331286136845No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12200.11670933253741395No Hit
ATCCTAGGTAATACTATAAATTGGTATCAGCAGGTCCCAGGAACGGCCCC11930.11412642107961873No Hit
AGTCTGTGCTGACTCAGCCACCCTCAACGTCTGGGACCCCCGGGCAGAGG11860.1134567773683385No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11800.11288279704438399No Hit
ACCTAGGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGTTGAGGGTGG11760.11250014349508097No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGGTGCAGTCTGAGGATG11750.11240448010775524No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC11710.11202182655845223No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11530.11029988558658875No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGGTCCCTTCATGCGTGACCTGG11460.10963024187530851No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11430.10934325171333126No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA11370.10876927138937678No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11200.10714299380483903No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTTAGAAACTATGCCATGAGCTGGGTCCG11190.1070473304175133No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10880.10408176541041507No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10880.10408176541041507No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG10720.10255115121320307No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10670.10207283427657433No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10660.10197717088924858No Hit
TCCGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCAA10570.10111620040331684No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10570.10111620040331684No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10550.10092487362866535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1000.044.90730341
TCGTATG1000.044.8422140
TCTGGAG14800.044.76450770
ATCTCGT955.456968E-1239.80964737
GCCGTCT1250.038.94563746
GTGGTAT1850.038.782491
CGGACTC1150.038.6514928
GACGGAC1150.038.62374526
TCTCGTA1009.094947E-1237.85014738
TATCTCG1009.094947E-1237.78277236
ACGAGAC1200.037.0020322
TATACAC1400.036.5989153
CAAGGGA10350.036.39845770
GTATCAA17450.035.829751
ACAGGTA1051.8189894E-1135.7924581
CTCCTAT1201.8189894E-1234.25732832
ACTCCTA1300.034.22776831
AGACGGA1300.034.1622625
CGAGACG1300.034.1557223
CACGAGA1300.034.15408721
Produced by FastQC (version 0.11.5)