FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045332
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43700.41804900261352373No Hit
GTAGTATACGGCCGTGTCGTCGGCTCTGAGACTGTTCATTTGCAGATACA39900.38169691542973905No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGGGACCC37820.36179893086598325No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGATGTCACTCCACG35620.34075298565431844No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32220.3082274339635637No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCAGCGTGGAGTGACATCCTGAATGGCTGGGTGTTCGGCGGAGGGAC28980.277232496470021No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26480.25331664963858375No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT26270.251307718504743No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGG25260.24164571638484234No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25220.2412630628355393No Hit
TCCTCAGACTGCACCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC24710.2363842300819261No Hit
GTATACTACTGTGCGAAAGGGCAGGTCTGGCGCGATTACTATTACTATTT23770.2273918716733057No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23620.22595692086341948No Hit
GTCTTCTCCACGGTGGTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23350.22337400940562424No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22940.21945181052526855No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22920.21926048375061702No Hit
GATTATTATTGTGCAGCGTGGAGTGACATCCTGAATGGCTGGGTGTTCGG22780.21792119632805654No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGGTCCCTTCA22670.2168688990674733No Hit
GTGCTGACTCAGCCACCCTCAACGTCTGGGACCCCCGGGCAGAGGGTCAC22150.21189440292653433No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21180.20261505435593669No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT21070.20156275709535343No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC20350.1946749932078995No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19500.18654360528521083No Hit
ATAATAATCAGCCTCATCCTCAGACTGCACCCCACTGATGGCCAGGGAGG19140.18309972334148383No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGGTGCAGT18550.17745558348926466No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18510.17707292993996165No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18350.17554231574274967No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18330.17535098896809817No Hit
CCCCTGAGGGCCGCCAATTATTACTATAGATGAGGAGTTTGGGGGCCGTT18320.17525532558077242No Hit
AGGTAATACTATAAATTGGTATCAGCAGGTCCCAGGAACGGCCCCCAAAC18010.1722897605736742No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17660.168941542017273No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17560.16798490814401548No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGAAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG17060.16320173877772803No Hit
GCCGTATACTACTGTGCGAAAGGGCAGGTCTGGCGCGATTACTATTACTA16020.15325274649585013No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15980.15287009294654713No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15710.1502871814887519No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15700.15019151810142614No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15630.14952187439014591No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15530.1485652405168884No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15210.14550401212246444No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15100.14445171486188121No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15090.14435605147455546No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14790.141486149854783No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14620.13985987227024524No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT14440.13813793129838176No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14370.13746828758710153No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13910.13306777177011705No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13750.13153715757290507No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG13670.13077185047429907No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13640.13048486031232182No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13580.12991087998836734No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGCCATTCAGGA13300.12723230514324638No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13120.1255103641713829No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12920.12359709642486788No Hit
CTCTTGTTCTGGAAGCAACTCCAACATCCTAGGTAATACTATAAATTGGTATCAGCAGGTCCCAGGAACGGCCCC12800.1224491357769589No Hit
GCCACATACCGCTCATCCGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCAAGAACACG12730.12177949206567866No Hit
GTATTACCTAGGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGACCCT12640.1209185215797469No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12520.11977056093183792No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12490.11948357076986067No Hit
CCCTCAACGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG12370.11833561012195169No Hit
GCACAATAATAATCAGCCTCATCCTCAGACTGCACCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG12350.1181442833473002No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12340.11804861995997444No Hit
GTATTAGTGGGAGTGGTGGTGCCACATACCGCTCATCCGCAGACTCCGTG12290.1175703030233457No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12270.1173789762486942No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12190.11661366915008821No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11960.11441341124159597No Hit
ATCCTAGGTAATACTATAAATTGGTATCAGCAGGTCCCAGGAACGGCCCC11930.11412642107961873No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT11810.11297846043170974No Hit
GTATACGGCCGTGTCGTCGGCTCTGAGACTGTTCATTTGCAGATACAGCG11790.11278713365705823No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11750.11240448010775524No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA11650.11144784623449776No Hit
TTATTGTGCAGCGTGGAGTGACATCCTGAATGGCTGGGTGTTCGGCGGAG11600.110969529297869No Hit
GTGGGGTGCAGTCTGAGGATGAGGCTGATTATTATTGTGCAGCGTGGAGT11570.11068253913589175No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11410.10915192493867977No Hit
GGATGTCACTCCACGCTGCACAATAATAATCAGCCTCATCCTCAGACTGC11310.10819529106542228No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11270.10781263751611929No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACTTTTAGAAACTATGCCATGAGCTGGGTCCG11110.10628202331890729No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGGTGCAGTCTGAGGATG11060.10580370638227855No Hit
ACCTAGGATGTTGGAGTTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGTTGAGGGTGG11040.10561237960762704No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10920.10446441895971806No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10790.10322079492448331No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC10760.10293380476250606No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC10590.10130752717796834No Hit
AGTCTGTGCTGACTCAGCCACCCTCAACGTCTGGGACCCCCGGGCAGAGG10560.10102053701599109No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10550.10092487362866535No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10510.10054222007936234No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGGGACCCCCGGGC10500.10044655669203659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)