FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003088
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66780.6657441819660886No Hit
ATATTATAGTAATCCTCGGACGTTCGGCCAAGGCACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT64280.6408211443063818No Hit
CTATAATATTGGTGGCAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC58030.5785135501571148No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57930.5775166286507266No Hit
GCTTAGGAGGCTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTATCAGTGTTGGAGCTGTATGAAATACT53450.5328545451645319No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT52010.5184988754725408No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51900.5174022618155136No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48630.48480292855661716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41660.41531749956135455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40220.40096182986936346No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38980.3886000031901488No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37840.3772350980173225No Hit
ATTTCATACAGCTCCAACACTGATAACTACTTAGCTTGGTACCAGCAGAA37750.3763378686615731No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37270.3715526454309094No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36520.3640757341329973No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35790.3567982071363629No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35440.35330898186400395No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA34630.3452339176622589No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34270.34164500023926114No Hit
GTTTATTACTGCCACCAATATTATAGTAATCCTCGGACGTTCGGCCAAGGCACCAAGGTGGAAATCAAACGAA33120.33018040291579603No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31690.3159244253744437No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30670.3057558260092833No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTGCCTTGGCCGAACGTC30650.30555644170800567No Hit
GCTCCAACACTGATAACTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG29710.2961853795479559No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29610.2951884580415677No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC29510.2941915365351794No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29500.29409184438454056No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29080.2899047740577098No Hit
GTGTTGGAGCTGTATGAAATACTCTGGCTGGACTTGCAGTTGATGGTGGC28800.28711339383982265No Hit
GTATGAAATACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA27240.27156141834016556No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTATCAGTGTTGGAGCTG27220.2713620340388879No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT27020.26936819102611137No Hit
AGCCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA26390.26308758553586525No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25550.25471344488220377No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25220.25142360391112245No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25020.2494297608983459No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG24570.2449436141195987No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGCTTCTGCTGGTA23600.23527347550763245No Hit
CTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC23430.23357870894677238No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23320.23248209528974528No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTCATA23300.2322827109884676No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22570.22500518399183322No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22470.22400826248544495No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22310.2224131880752237No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21990.21922303925478123No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC21680.21613258258497756No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGCCACCAATATTATAGTAAT21470.21403904742156224No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21320.2125436651619798No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20420.20357137160448535No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20220.20157752859170883No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTCATACAGCTCCAA19820.1975898425661557No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19550.19489815449890735No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGCTTCTGCTGGTACCAAGCTA19420.19360215654060262No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19020.18961447051504954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18930.18871724115930008No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18930.18871724115930008No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18370.18313448072352576No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18270.18213755921713748No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18160.18104094556011038No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTCATACAGCTCCAACA18140.18084156125883274No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18110.18054248480691623No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT17970.17914679469797265No Hit
GGCTGAAGATGTGGCAGTTTATTACTGCCACCAATATTATAGTAATCCTC17900.1784489496435009No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17870.1781498731915844No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17820.17765141243839025No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17480.17426187931667012No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17350.17296588135836535No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17350.17296588135836535No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC16820.1676821973745075No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16610.16558866221109214No Hit
GCTAAGTAGTTATCAGTGTTGGAGCTGTATGAAATACTCTGGCTGGACTT16540.16489081715662038No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16280.16229882124001085No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTGCCTTGGCCGAACGTCCGAGGATTA15890.1584108273650966No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15800.15751359800934714No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15790.15741390585870832No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15770.15721452155743065No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15740.15691544510551417No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15610.1556194471472094No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15580.15532037069529292No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15430.15382498843571055No Hit
ATAGTAATCCTCGGACGTTCGGCCAAGGCACCAAGGTGGAAATCAAACGA15350.1530274512305999No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGCCACCAATATTATAGTAATCCTCGGACGTT15270.1522299140254893No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTCATACAGCTCCAACACTGATAACTA15190.1514323768203787No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15070.15023607101271275No Hit
GATAACTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCTAAGCT14940.14894007305440798No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG14930.14884038090376916No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT14820.14774376724674207No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14820.14774376724674207No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14690.1464477692884373No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14550.14505207917949373No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14240.14196162250969008No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14080.14036654809946883No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13800.1375751678815817No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA13680.13637886207391575No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13540.13498317196497217No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13420.13378686615730626No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13400.1335874818560286No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG13230.13189271529516852No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG13100.13059671733686376No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13070.1302976408849473No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13010.1296994879811143No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12870.12830379787217072No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC12820.1278053371189766No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG12780.1274065685164213No Hit
GAGTATTTCATACAGCTCCAACACTGATAACTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCTAAG12760.12720718421514363No Hit
GTACCAGCAGAAGCCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA12410.12371795894278467No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT12310.12272103743639641No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC12160.121225655176814No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT12120.12082688657425869No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTGCCTTGGCCGAACGTCCGAGG12060.12022873367042572No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT11830.1179358142057327No Hit
GCACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11670.11634073979551146No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA11530.11494504968656788No Hit
CCTTGGTGCCTTGGCCGAACGTCCGAGGATTACTATAATATTGGTGGCAGTAATAAACTGCCACATCTTCAGC11490.11454628108401256No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTGCCTTGGC11480.11444658893337374No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA11240.11205397731804188No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTGC11120.11085767151037597No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGG11060.11025951860654301No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10740.10706936978610053No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10720.10686998548482286No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10560.10527491107460163No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC10510.10477645032140749No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10500.10467675817076867No Hit
GTATTTCATACAGCTCCAACACTGATAACTACTTAGCTTGGTACCAGCAGAAGCCAGGACAGCCTCCTAAGCTG10480.104477373869491No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG10450.10417829741757453No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG10410.10377952881501923No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG10380.10348045236310274No Hit
GTAGTTATCAGTGTTGGAGCTGTATGAAATACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGA10350.10318137591118624No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10350.10318137591118624No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT10320.10288229945926977No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG10200.10168599365160386No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA10090.10058937999457675No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGCTTCTG10090.10058937999457675No Hit
CTGCTGGTACCAAGCTAAGTAGTTATCAGTGTTGGAGCTGTATGAAATAC10090.10058937999457675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA305.9179583E-446.60410349
AATGGTA3550.041.8974270
GGTATCA11350.037.0670661
GTATCAA23100.035.984591
GTGGTAT2750.034.5452041
GACAGGT1155.820766E-1132.4531821
TGCTTAA450.00429741331.1269650
GGTCTGG26650.030.44226870
TACTGGC450.00502091530.1524982
TATCAAC27750.029.8265272
ATCAACG28000.029.4376033
TCAACGC28200.029.3491084
TTGCCGT853.8579947E-628.91857752
AACGCAG28800.028.7462656
CAACGCA28800.028.7391015
ACGCGCC656.021247E-428.23887666
GCCTGAC5550.028.1291897
CTAGGGA500.007257205427.96246149
TGCTAAC1104.2698957E-827.78447511
ACAAGGT500.00790490427.47694236
Produced by FastQC (version 0.11.5)