FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908725
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAAGTCTGTTGACAGTAATAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC43430.47792236375140995No Hit
GACTTATAGTGAGCCGTTCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAACTGTGGCTGCACCAT43110.47440094638091834No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31640.34818014250735924No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27930.3073537098682219No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22080.24297779856392196No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21520.23681531816556164No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18980.2088640677872844No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18580.20446229607416988No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17160.18883600649261328No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17100.1881757407356461No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16580.18245343750859722No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16050.17662108998872045No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15910.17508046988913037No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15490.1704586095903601No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15350.16891798949077003No Hit
GTAATAGACGGCCGTATCGTCAGATGTCAGTCTGCTCAATTCCATGTAGACTGTCGACGTGGACATGTCCCTGGT15010.1651764835346227No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGTCCCAGTC14690.16165506616413106No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14390.15835373737929517No Hit
CTCCTGATCTATGCGGCATCCAATTTGCAAAGTGGCGTCCCGTCAAGGTT14180.15604280722991004No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13900.15296156703072986No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13590.14955019395306612No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13130.1444881564829844No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12920.14217722633359928No Hit
GATCAGGAGCTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAATGTAAAT12790.14074665052683705No Hit
GTTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTC12400.13645492310655039No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTATTACTGTCAACAGACTTATAGTGAG12310.13546452447109963No Hit
GTGGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGAACTTACATCTATTTACATTGGTATCAGCAGAAA12200.13425403724999313No Hit
GTGCAGCCACAGTTCGATTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG12130.13348372720019808No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT12050.13260337285757517No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11920.13117279705081294No Hit
GTCTCCATCCTCCCTGTCCGCATCTGTGGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGAACTTACAT11910.1310627527579851No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11830.13018239841536217No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11640.1280915568516328No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11330.12468018377396901No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11180.12302951938155107No Hit
ATAGTGAGCCGTTCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGA11100.12214916503892816No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTATTA11020.12126881069630525No Hit
GTCCGCATCTGTGGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA10490.11543646317642851No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGTCCCAGTCTCCATCCTCC10430.11477619741946134No Hit
CCTTGGTCCCTCCGCCGAAAGTGAACGGCTCACTATAAGTCTGTTGACAG10080.11092464717048613No Hit
GTCTATTACTGTGCGAGAAGCCACAGCCCCGATAGTAGTGCTCATCTCCACTGGGGCCAGGGAACCCTGGTGT9930.10927398277806818No Hit
GATCTATGCGGCATCCAATTTGCAAAGTGGCGTCCCGTCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCA9870.10861371702110098No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9750.10729318550716663No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9620.10586260970040441No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGAACTTACATCTATTTACATTGGTATCAGCAGAAACCAGGGAGAG9550.10509229965060937No Hit
ACTTATTACTGTCAACAGACTTATAGTGAGCCGTTCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAA9410.1035516795510193No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTGACGGGACGCCA9380.10322154667253569No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9380.10322154667253569No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9260.10190101515860134No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9260.10190101515860134No Hit
GCTCACTATAAGTCTGTTGACAGTAATAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT9160.1008005722303227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2600.049.06402640
GACCGAT3900.047.78314226
ACCGATC3950.046.35627727
CGTATGC2600.045.21011741
TATCTCG3350.044.97852736
AGACCGA4200.044.34562325
AGTATCG551.5891419E-743.47315634
TATACAC4450.043.326143
ACGAGAC4300.043.27141622
ATCTCGT3500.043.1253537
TATCGCG405.2249154E-542.80626736
GTATCGC405.2749245E-542.7372235
CCGATCA4300.042.6136528
ATCTCCG4400.041.51224510
CCCACGA4500.041.3414119
CGATCAG4500.040.7489529
TACACAT4500.040.5897525
GAGACCG4600.040.47387324
CGAGACC4650.040.0232423
CCGTCTT2800.039.96768647
Produced by FastQC (version 0.11.5)