FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908725
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47320.5207295936614487No Hit
GACTTATAGTGAGCCGTTCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAACTGTGGCTGCACCAT43010.4733005034526397No Hit
CTATAAGTCTGTTGACAGTAATAAGTCGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC41650.4583344796280503No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30520.3358551817106385No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22000.24209744422129909No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21440.23593496382293874No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19880.2187680541417921No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17360.19103689234917054No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17110.18828578502847396No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16870.18564472200060525No Hit
GTGGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGAACTTACATCTATTTACATTGGTATCAGCAGAAA16620.18289361467990867No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15980.17585077993892542No Hit
GTAATAGACGGCCGTATCGTCAGATGTCAGTCTGCTCAATTCCATGTAGACTGTCGACGTGGACATGTCCCTGGT15640.17210927398277806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15470.17023852100470438No Hit
CTCCTGATCTATGCGGCATCCAATTTGCAAAGTGGCGTCCCGTCAAGGTT15000.16506643924179482No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14350.15791356020798372No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14300.1573633387438444No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13980.15384192137335276No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGTCCCAGTC13280.14613882087540234No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13230.14558859941126304No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTATTACTGTCAACAGACTTATAGTGAG13090.14404797931167296No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13060.14371784643318936No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12970.14272744779773858No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12930.14228727062642715No Hit
GTTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTC12860.1415169605766321No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12720.13997634047704202No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12560.1382156317917962No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11860.13051253129384577No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCGACTTATTA11810.12996230982970644No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11380.12523040523810833No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11090.12203912074610032No Hit
GATCAGGAGCTTAGGGGCTCTCCCTGGTTTCTGCTGATACCAATGTAAAT11040.12148889928196098No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11000.12104872211064954No Hit
GTGCAGCCACAGTTCGATTGATGTCCACCTTGGTCCCTCCGCCGAAAGTG10820.119067924839748No Hit
GTCTCCATCCTCCCTGTCCGCATCTGTGGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGAACTTACAT10560.11620677322622355No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGTCCCAGTCTCCATCCTCC10450.11499628600511705No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10380.11422597595532201No Hit
GTCTATTACTGTGCGAGAAGCCACAGCCCCGATAGTAGTGCTCATCTCCACTGGGGCCAGGGAACCCTGGTGT10280.1131255330270434No Hit
GTCCGCATCTGTGGGGGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA10090.11103469146331398No Hit
GATCTATGCGGCATCCAATTTGCAAAGTGGCGTCCCGTCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCA9770.10751327409282237No Hit
ATAGTGAGCCGTTCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGA9490.1044320338936422No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTGACGGGACGCCA9360.10300145808687998No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9360.10300145808687998No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9270.1020110594514292No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9220.10146083798728989No Hit
ACTTATTACTGTCAACAGACTTATAGTGAGCCGTTCACTTTCGGCGGAGGGACCAAGGTGGACATCAATCGAA9150.10069052793749485No Hit
GTGGCGTCCCGTCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCACT9130.10047043935183912No Hit
CCTTGGTCCCTCCGCCGAAAGTGAACGGCTCACTATAAGTCTGTTGACAG9130.10047043935183912No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9090.10003026218052766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT300.002531675667.7497870
CTAACGC353.802179E-757.9082533
GTGGTAT2250.039.064551
CCTAACG502.10123E-433.7946932
GTATCAA17100.033.2127761
TATCAAC20350.027.567172
TAACGCT500.00857242927.023854
CAACGCA20750.026.6967955
ATCAACG20950.026.6046283
TCAACGC21000.026.3804264
AACGCAG21350.025.9465316
AACGGCC2050.025.09254537
GACAGGT1951.8189894E-1224.2708741
ACGCAGA22750.024.201357
TTGCGCG700.001504626424.13773518
AATCTGT14650.023.78139768
TCGACGT5700.023.67420654
AGAGTAC23450.023.47762711
TGTCGAC5750.023.35350252
GCAGAGT23950.022.9862239
Produced by FastQC (version 0.11.5)