FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186346
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA37090.31264066301062254No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35900.30260986255274597No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG35390.2983109480707989No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT34720.2926633545356919No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG30240.25490034104721554No Hit
GTCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGGTTAT29530.24891557774881864No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCACTGTCCCACACCTGACAGTAATAGT29040.24478524814851654No Hit
GTCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGC28700.2419193051605518No Hit
GTGTATTACTGCGTGGTAGCTGCTAGCGTCGCGTTATCAACTTTTGATAT28580.24090779587068192No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAGTTCCAATGTTGTTTCCCCCACAGATAA28270.23829473020518466No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27010.2276738826615507No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26720.2252294018776984No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC26490.2232906757387811No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26220.2210147798365738No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT25400.21410279968912949No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24740.208539498594845No Hit
GGGTTATCTGTGGGGGAAACAACATTGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC24600.20735940442333012No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGGTATCCCT23890.20137464112493322No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCAC23400.19724431152463112No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22460.18932082208731685No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCACTGTCCCACACCTGA22120.18645487909935213No Hit
GCCCTCAGGTATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG21870.18434756807878983No Hit
CCCCAGGACAGACGGCCAGGGTTATCTGTGGGGGAAACAACATTGGAACT21490.18114445532753515No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21420.18055440824177768No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21010.17709841816805552No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20640.17397959785762332No Hit
GGGATACCTGAGGGCCGGGCGCTATCATCATAGACGACCAGCACAGGGGC20150.16984926825732122No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20080.16925922117156378No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAA19330.16293728810987687No Hit
CACTACCACTGTCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGG19250.16226294858329696No Hit
GTGGTAGTGATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA19200.16184148637918447No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19200.16184148637918447No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18560.156446770166545No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG18490.15585672308078757No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACT18260.15391799694187025No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA18150.15299078009282283No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTAC18110.15265361032953287No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18050.15214785568459793No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18040.15206356324377543No Hit
GTGTGGGACAGTGGTAGTGATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC18040.15206356324377543No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18000.15172639348048547No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17870.15063059174979307No Hit
CTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG17780.14987195978239065No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGA17480.1473431865577159No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17340.14616309238620098No Hit
CCTGAGGGCCGGGCGCTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACA17270.14557304530044354No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17250.14540446041879856No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17130.14439295112892866No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTG16970.1430442720757688No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16910.14253851743083384No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16830.1418641779042539No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16660.14043120641027154No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16600.13992545176533658No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16540.13941969712040164No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16530.13933540467957914No Hit
GTCCACATAGTATTCGTCGCTTCCATCTTCGTTTATGTTGGCCACCCACT16500.13908252735711168No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16500.13908252735711168No Hit
CTCCAGCCCCTTCTCTGGAGCCTGGCGGACCCAGCTCATCCAAGAGGCAC16400.13823960294888674No Hit
CAGGTATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC16350.13781814074477428No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16340.1377338483039518No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAGGCCGGGGATGAGGCCGACTAT15770.13292917917706976No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATACCTGAGGGCCGGGCGCTATCATCATAGA15580.13132762280144242No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGCCTCTTGGATGAGCTGGGTCCGCCAGGCTCCAGAGAAGGGG15580.13132762280144242No Hit
CTTTTAGTTCCAATGTTGTTTCCCCCACAGATAACCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG15400.12981035886663755No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGG15370.12955748154417007No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC15180.12795592516854273No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14870.1253428595030455No Hit
CCTCTCTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACTCAGCCAC14830.1250056897397555No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC14800.12475281241728804No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14690.12382559556824062No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14590.12298267116001571No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14520.12239262407425827No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14370.12112823746192089No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACTCAGC14270.12028531305369597No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG14130.11910521888218108No Hit
GTATTACTGCGTGGTAGCTGCTAGCGTCGCGTTATCAACTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT14020.11817800203313368No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13670.11522776660434644No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13580.11446913463694403No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13270.11185606897144679No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13180.11109743700404434No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13150.11084455968157687No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13040.10991734283252946No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG12900.10873724866101457No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12830.10814720157525713No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGACCTCCTA12790.10781003181196717No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC12790.10781003181196717No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC12690.10696710740374225No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12550.10578701323222736No Hit
CAGATAACCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGGCTG12430.10477550394235745No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12360.10418545685660001No Hit
GTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGG12280.10351111733002008No Hit
CAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGT12150.10241531559932768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)