FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186346
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47160.3975231509188719No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA33300.2806938279388981No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT33150.2794294413265607No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32650.2752148192854361No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG31240.26332958512946475No Hit
GTCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGGTTAT30390.25616472765955295No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC29590.2494213323937536No Hit
GGGTTATCTGTGGGGGAAACAACATTGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC28250.23812614532353968No Hit
GTCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGC28220.2378732680010722No Hit
GTGTATTACTGCGTGGTAGCTGCTAGCGTCGCGTTATCAACTTTTGATAT27750.23391152328241507No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCACTGTCCCACACCTGACAGTAATAGT27720.2336586459599476No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG27530.23205708958432025No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25390.214018507248307No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAGTTCCAATGTTGTTTCCCCCACAGATAA24920.2100567625296499No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24720.20837091371320002No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24390.2055892631660578No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGGTATCCCT23080.19454695341831135No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22880.1928611046018615No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22780.1920181801936366No Hit
GTGGTAGTGATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA22640.1908380860221217No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCACTGTCCCACACCTGA22270.1877192657116895No Hit
GCCCTCAGGTATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG21180.17853138966203788No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21090.17777275769463546No Hit
GTGTGGGACAGTGGTAGTGATTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC20860.17583403155571814No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20340.17145082463294856No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATACCTGAGGGCCGGGCGCTATCATCATAGA20230.17052360778390116No Hit
GGGATACCTGAGGGCCGGGCGCTATCATCATAGACGACCAGCACAGGGGC20230.17052360778390116No Hit
CCCCAGGACAGACGGCCAGGGTTATCTGTGGGGGAAACAACATTGGAACT20150.16984926825732122No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACT20030.16883775896745132No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTACCAC19960.16824771188169385No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19150.16142002417507204No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19050.16057709976684711No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAATCACTAC18950.1597341753586222No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18250.15383370450104777No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18130.15282219521117785No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17790.14995625222321313No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17670.14894474293334323No Hit
CACTACCACTGTCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCCTCGACCCTGCTGATGGTCAGGGTGG17390.14658455459031344No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17360.14633167726784596No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGA17000.14329714939823626No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16970.1430442720757688No Hit
CTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG16790.14152700814096394No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAA16520.13925111223875666No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16520.13925111223875666No Hit
GTCCACATAGTATTCGTCGCTTCCATCTTCGTTTATGTTGGCCACCCACT16400.13823960294888674No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16380.13807101806724176No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16340.1377338483039518No Hit
CCTGAGGGCCGGGCGCTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACA16190.13646946169161442No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16160.13621658436914694No Hit
CTCCAGCCCCTTCTCTGGAGCCTGGCGGACCCAGCTCATCCAAGAGGCAC16150.13613229192832443No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16110.13579512216503448No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGCCTCTTGGATGAGCTGGGTCCGCCAGGCTCCAGAGAAGGGG16110.13579512216503448No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG15870.13377210358529468No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTG15860.13368781114447217No Hit
CAGGTATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC15820.13335064138118222No Hit
CTTTTAGTTCCAATGTTGTTTCCCCCACAGATAACCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG15730.1325920094137798No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15670.13208625476884484No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15570.13124333036061991No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGCCCGGCCCTCAGG15420.12997894374828253No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15210.12820880249101022No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15070.12702870831949534No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15040.12677583099702786No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC14980.12627007635209292No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG14750.1243313502131756No Hit
GTATTACTGCGTGGTAGCTGCTAGCGTCGCGTTATCAACTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGT14690.12382559556824062No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14670.12365701068659564No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACTCAGC14610.12315125604166069No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14450.12180257698850083No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14440.12171828454767833No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG14270.12028531305369597No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14240.1200324357312285No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC14200.11969526596793854No Hit
CCTCTCTCACTGCACAGTCTCTGTGACCTCCTATGTGCTGACTCAGCCAC14160.11935809620464854No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAGGCCGGGGATGAGGCCGACTAT13750.1159021061309264No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG13680.11531205904516893No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13630.11489059684105649No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13570.11438484219612154No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13540.11413196487365407No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13470.11354191778789663No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13400.11295187070213916No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13290.11202465385309177No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGACCTCCTA13230.11151889920815682No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13170.11101314456322185No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC12800.10789432425278966No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12620.1063770603179848No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12570.10595559811387234No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTGTGACCTC12490.10528125858729241No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12360.10418545685660001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGA200.007280230551.89111343
TTCGGCG200.00795033450.74820331
GGCTAAT307.0902455E-444.92648327
GTATCAA14550.041.8134461
GGTATCA5250.041.6154371
GTGGTAT1500.035.85331
GTAAGCA400.00285750933.83213831
TTAGGGT400.002953885633.6039664
ATCAACG18100.033.232653
TCAACGC18550.032.4264684
CAACGCA18700.032.1704335
AACGCAG18900.032.0132266
GTATAAG652.7741542E-531.0268921
ACGCAGA19600.030.8737987
GTGCAAC553.794722E-430.5567871
CGCAGAG19800.030.3972848
TGGTATC1900.030.0679722
AGAGTCG450.005234899529.89665411
GGATCGA450.005237059729.89413519
GCTTGGG1351.2732926E-1129.8777481
Produced by FastQC (version 0.11.5)