FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783361
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28650.3657317635164375No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27490.35092377588366025No Hit
CTATTATACTCCAGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTC26860.34288150673827267No Hit
GTATAATAGTTGGCCCCCCCTCTACACTTTTGGCCAGGGGACCAAGCTGG24210.3090529143013247No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20390.2602886791657997No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19810.2528846853494111No Hit
GTTAGGGCCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA18920.24152338449322852No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18690.23858731797983304No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18250.23297049508464168No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16950.2163753365306672No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16820.21471582067526976No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15570.1987589374502943No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14880.18995073791010786No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14490.18497219034391552No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGTAACAACTTAGCCTGGTACCAGCAGAAACCTGACCAGGCCCCCAGACT14150.1806319181067222No Hit
GTTTATTACTGTCTGGAGTATAATAGTTGGCCCCCCCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA13980.1784617819881255No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13730.17527040534313043No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT12950.16531331021074575No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12760.16288786396054947No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12700.16212193356575064No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT12590.16071772784195282No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12270.1566327657363591No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12160.15522856001256127No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12060.1539520093545632No Hit
GTCTCCAGCCACCGCGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGTAA11900.15190952830176635No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11540.14731394593297342No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11540.14731394593297342No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11280.14399491422217853No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT10990.14029291731398424No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10910.13927167678758579No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10910.13927167678758579No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10330.1318676829711972No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10070.1285486512604023No Hit
GTGTAGAGGGGGGGCCAACTATTATACTCCAGACAGTAATAAACTGCAAA10030.12803803099720307No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10010.12778272086560347No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9830.125484929681207No Hit
CCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG9700.12382541382580955No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGAGGGGGGG9570.12216589797041211No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9360.11948514158861623No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9210.11757031560161917No Hit
CCCCCAGACTCCTCATCTATGATGGTTCCACCAGGGCCACTGGTATCCCA9140.11667673014102056No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9030.11527252441722272No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8990.11476190415402349No Hit
GCTCAGTTAGGGCCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8940.11412362882502447No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8840.11284707816702644No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8730.1114428724432286No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGTAACAACTTAGCCTGGTACCAGCAGAAAC8710.11118756231162899No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA8560.10927273632463194No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCTGGAGTATAATAGTTGG8480.10825149579823351No Hit
ATTATACTCCAGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGC8450.10786853060083411No Hit
GGATACCAGTGGCCCTGGTGGAACCATCATAGATGAGGAGTCTGGGGGCCTGGTCAGGTTTCTGCTGGTACCAGG8410.10735791033763488No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8410.10735791033763488No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8340.10646432487703625No Hit
GTCAGAGTCTTAGTAACAACTTAGCCTGGTACCAGCAGAAACCTGACCAG8320.10620901474543666No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8270.10557073941643765No Hit
GAGTATAATAGTTGGCCCCCCCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA8210.10480480902163881No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8150.10403887862684No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA8050.10276232796884194No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8010.10225170770564274No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8000.10212405263984292No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG7980.10186874250824333No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGTAACAACTTAGCCTGGTACCAGCAGAAACCTGACCAGG7960.10161343237664372No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7890.10071984691604509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAG150.00252391267.80694617
CGTATGG200.00733515951.7909141
TCGTATG1300.050.35163540
GGTATCA9400.049.415661
GACGTAG1600.042.4362326
ATCTCGT1300.042.198837
CACGAGA1550.041.56440421
AAGCGGT806.660365E-541.07350570
CGAGACG1650.039.0503323
CCCACGA1650.039.0403619
CGTAGAG1750.038.84105328
ACGTAGA1750.038.8286327
AGACGTA1750.038.78148725
CGTATGC1450.038.0990641
ATGCCGT1300.037.3727444
GTATCAA23800.037.324691
ACGAGAC1750.036.81418622
ATCTCCG1850.036.65006610
TACGTAC754.6848072E-836.16370412
GCGATTT1101.0312386E-535.8459770
Produced by FastQC (version 0.11.5)