FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783361
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTGGCCCCCCCTCTACACTTTTGGCCAGGGGACCAAGCTGG27220.3474770891070656No Hit
CTATTATACTCCAGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTC27100.34594522831746793No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26120.3334350318690872No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23810.3039467116693325No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18660.23820435278243363No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18580.23718311225603522No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17890.22837491271584875No Hit
GTTAGGGCCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA17820.22748132725525014No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17170.2191837479782629No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16610.21203506429347388No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14870.18982308284430804No Hit
GTTTATTACTGTCTGGAGTATAATAGTTGGCCCCCCCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA14570.18599343087031395No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14440.18433391501491647No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14340.18305736435691847No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGTAACAACTTAGCCTGGTACCAGCAGAAACCTGACCAGGCCCCCAGACT14020.17897240225132474No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13890.1773128863959273No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT13770.17578102560632966No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13640.17412150975093219No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13550.17297261415873397No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13290.16965358244793907No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13160.16799406659254162No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12820.1636537943553483No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12670.16173896836835125No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12490.15944117718395479No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT12470.15918586705235516No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12380.15803697146015694No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12180.15548387014416085No Hit
GTCTCCAGCCACCGCGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGTAA11230.1433566388931795No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11040.14093119264298326No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10690.13646326533999012No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT10520.13429312922139347No Hit
GTGTAGAGGGGGGGCCAACTATTATACTCCAGACAGTAATAAACTGCAAA10290.13135706270799796No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10210.13033582218159953No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10080.12867630632620208No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9880.12612320501020602No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9820.1253572746154072No Hit
CCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG9700.12382541382580955No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9550.1219105878388125No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9510.12139996757561328No Hit
ATTATACTCCAGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGC9290.11859155612801761No Hit
GAGTATAATAGTTGGCCCCCCCTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA9280.1184639010622178No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGTAACAACTTAGCCTGGTACCAGCAGAAAC9210.11757031560161917No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCTGGAGTATAATAGTTGG9090.11603845481202155No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8620.11003866671943077No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA8560.10927273632463194No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8440.1077408755350343No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8430.1076132204692345No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGAGGGGGGG8370.10684729007443566No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8330.10633666981123646No Hit
GCTCAGTTAGGGCCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8220.10493246408743863No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGTAACAACTTAGCCTGGTACCAGCAGAAACCTGACCAGG8110.10352825836364077No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA8050.10276232796884194No Hit
GTCAGAGTCTTAGTAACAACTTAGCCTGGTACCAGCAGAAACCTGACCAG8040.10263467290304215No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8020.10237936277144254No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7970.10174108744244352No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA7960.10161343237664372No Hit
CTGCTACTCTGGCTCCCAGATATCACTGGAGAAGTCGTGATGACGCAGTC7880.10059219185024529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)