FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175793
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTACCTCACCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT58790.5000029767144387No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56870.48367357179367454No Hit
GTACCATACTGCTGACAATAATATACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTAAGAGTGAAGTCTGTC46310.3938618447294719No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43700.37166405991530826No Hit
ATATTATTGTCAGCAGTATGGTACCTCACCGTCCACTTTTGGCCAGGGGA38610.32837412707849084No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA37350.31765795509923944No Hit
GTACCTCACCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA36590.3111942323181036No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34380.2923984068624324No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32770.27870552044450003No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32560.27691949178129144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31920.2714763568077034No Hit
GTGAGGTACCATACTGCTGACAATAATATACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTAAGAGTGAAGT31790.27037072001619333No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28880.2456214656831602No Hit
GTCTGGGACAGACTTCACTCTTACCATCAGCAGAGTGGAGCCTGAAGATT28640.24358029006806473No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCCGGGATGCCAGTGGCCCTGCG27620.23490529370390878No Hit
CATATATGGTGATTCCCGCAGGGCCACTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA27380.23286411808881324No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27320.2323538241850394No Hit
CAATAATATACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTAAGAGTGAAGTCTGTCCCAGACCCACTGCCA27180.23116313840956698No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAT27120.2306528445057931No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCATCATCTACTTAGCCTGGTACC26480.2252097095322051No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25940.22061706439824016No Hit
CCTCATATATGGTGATTCCCGCAGGGCCACTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT25860.21993667252654167No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24070.20471290439728762No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC23810.20250163081426747No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23450.1994398673916242No Hit
GTCTTAGCATCATCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATATATGGTGATT23240.19765383872841566No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22960.19527246717747088No Hit
CCATATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGATGATGCTAAGACTCTGA22710.19314624257841304No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22380.1903396261076567No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21600.1837058053585963No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21050.17902811124066906No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20910.17783742546519668No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20870.17749722952934743No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG20550.1747756620425534No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20480.17418031915481721No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19620.16686610653405828No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19590.16661095958217137No Hit
GTATATTATTGTCAGCAGTATGGTACCTCACCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA19240.16363424514349037No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCCGGGATGCCA18620.15836120813782698No Hit
GACTTCACTCTTACCATCAGCAGAGTGGAGCCTGAAGATTTTGCAGTATATTATTGTCAGCAGTATGGTACCTCA18250.1552143957312214No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17920.15240777926046506No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17920.15240777926046506No Hit
ATAATATACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTAAGAGTGAAGTCTGTCCCAGACCCACTGCCA17520.14900581990197254No Hit
TATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAA17450.14841047701423635No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17120.14560386054348No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17050.14500851765574382No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17020.14475337070385688No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16860.14339258696045987No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16750.14245704813687443No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16620.14135141134536436No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16440.13982052963404273No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16110.1370139131632864No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15720.13369700278875618No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15570.1324212680293215No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15460.13148572920573606No Hit
GTATCAACGCAGAGTACGGGAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCT14520.12349112471327862No Hit
GTCAGAGTCTTAGCATCATCTACTTAGCCTGGTACCAGCAGAAACCTGGC14500.12332102674535397No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAGATTGTGTTGACGCAGTC14140.12025926332271072No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13980.11889847957931371No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13830.11762274481987901No Hit
CTCCCAGGCTCCTCATATATGGTGATTCCCGCAGGGCCACTGGCATCCCG13820.1175376958359167No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13760.11702740193214281No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13640.11600681412459507No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAGATTGTGTTGACGCAGTCTC13460.11447593241327343No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13370.1137104915576126No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13150.11183941391044172No Hit
GAGTACGGGAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCC13060.1110739730547809No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12770.10860755251987382No Hit
CATCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATATATGGTGATTCCCGCAGGGC12530.1065663769047783No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12320.10478034824156973No Hit
GGCCAAAAGTGGACGGTGAGGTACCATACTGCTGACAATAATATACTGCA12140.1032494665302481No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGAGGAATCATCCCTTTCCTTCGT12120.10307936856232347No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12000.1020587807547757No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATATATGGTGATTCCCGCAGGGCCACTGGCATCCCGGACAGGTT11860.10086809497930334No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11840.10069799701137871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)