FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175793
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56880.48375862077763687No Hit
GTATGGTACCTCACCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT54900.46691892195309886No Hit
GTACCATACTGCTGACAATAATATACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTAAGAGTGAAGTCTGTC42380.3604375940322829No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36640.31161947723791517No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA36600.3112792813020659No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36360.3092381056869704No Hit
ATATTATTGTCAGCAGTATGGTACCTCACCGTCCACTTTTGGCCAGGGGA35810.3045604115690432No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33690.2865300269690328No Hit
GTACCTCACCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA32790.27887561841242464No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32650.27768493263695226No Hit
GTGAGGTACCATACTGCTGACAATAATATACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTAAGAGTGAAGT30650.26067513584448965No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29160.248002837234105No Hit
GTCTGGGACAGACTTCACTCTTACCATCAGCAGAGTGGAGCCTGAAGATT29040.24698224942655725No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27910.23737171423881587No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCATCATCTACTTAGCCTGGTACC26600.22623029733975283No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCCGGGATGCCAGTGGCCCTGCG25970.2208722113501271No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAT25470.21661976215201142No Hit
CAATAATATACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTAAGAGTGAAGTCTGTCCCAGACCCACTGCCA25120.2136430477133305No Hit
CCTCATATATGGTGATTCCCGCAGGGCCACTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT24910.21185701905012191No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24770.2106663332746495No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24610.20930554953125252No Hit
CATATATGGTGATTCCCGCAGGGCCACTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA24540.20871020664351633No Hit
GTCTTAGCATCATCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATATATGGTGATT24320.20683912899634543No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24200.2058185411887977No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23940.20360726760577758No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23440.1993548184076619No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC23250.19773888771237794No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG23070.1962080060010563No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21610.1837908543425586No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21600.1837058053585963No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20780.1767317886736866No Hit
CCATATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGATGATGCTAAGACTCTGA20700.1760513968019881No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20440.17384012321896797No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19750.16797174332556836No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19090.1623585103840557No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18850.16031733476896018No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18550.1577658652500908No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18530.15759576728216618No Hit
GTATATTATTGTCAGCAGTATGGTACCTCACCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18520.15751071829820384No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCCGGGATGCCA18430.15674527744254302No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18210.15487419979537215No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18120.15410875893971132No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17360.14764503615857552No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17240.14662444835102778No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17100.1454337625755554No Hit
GACTTCACTCTTACCATCAGCAGAGTGGAGCCTGAAGATTTTGCAGTATATTATTGTCAGCAGTATGGTACCTCA17060.14509356663970616No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16820.14305239102461062No Hit
ATAATATACTGCAAAATCTTCAGGCTCCACTCTGCTGATGGTAAGAGTGAAGTCTGTCCCAGACCCACTGCCA16790.14279724407272368No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16340.1389700397944196No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16100.1369288641793241No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC16030.1363335212915879No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15430.1312305822538491No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAGATTGTGTTGACGCAGTC15390.13089038631799985No Hit
TATATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAA15200.12927445562271592No Hit
GTATCAACGCAGAGTACGGGAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCT15180.12910435765479128No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14990.12748842695950732No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14460.12298083080950473No Hit
GTCAGAGTCTTAGCATCATCTACTTAGCCTGGTACCAGCAGAAACCTGGC14450.12289578182554242No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14390.12238548792176854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14130.12017421433874839No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13750.11694235294818052No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAGATTGTGTTGACGCAGTCTC13470.11456098139723574No Hit
GAGTACGGGAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCC13030.11081882610289395No Hit
CTCCCAGGCTCCTCATATATGGTGATTCCCGCAGGGCCACTGGCATCCCG13010.11064872813496933No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12780.10869260150383614No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12770.10860755251987382No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12480.10614113198496673No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12220.1039298584019466No Hit
GGCCAAAAGTGGACGGTGAGGTACCATACTGCTGACAATAATATACTGCA12160.10341956449817272No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11940.10154848685100183No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGC11780.10018770310760482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23950.036.087331
TATCAAC26150.032.6539842
ATCAACG26900.031.7435593
TCAACGC26800.031.7337134
ATGGCAA3550.031.64971270
CAACGCA27650.030.7607945
GGTATCA13950.030.4903451
AACGCAG28100.030.3931316
TTAATCG802.809522E-630.12105837
AGAGTAC29600.028.99749611
CGCAGAG29900.028.581688
ACGCAGA29900.028.573177
TAATCGT854.4701974E-628.3773238
GATTCAC2950.027.6831651
TTCCGCA1005.606398E-727.2519219
CAGTTCG20150.026.89601770
CGTAGGA907.509094E-626.54579428
ATTCACT2900.025.8082682
GAGTTTA3550.024.9213471
TACGTAC2350.024.63954512
Produced by FastQC (version 0.11.5)