FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750862
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC76110.43470016483309365No Hit
CTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAG75480.43110193721721074No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCA75250.4297882985637932No Hit
CACCTGCACCTTGCCCAGTGACATCAATGTTGGTACCTCCAACATATACTGGTACCAACAGAAGCCAGGGAGCC71390.4077420150760026No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCGGAGATGAGTAAGATCC70990.4054574261135372No Hit
CTACTACTCAGACTCAGATAAGCGCCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGC68620.39192123651093No Hit
GACTATTACTGTATGATTTGGCCAGCCAATACTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG58440.333778447416187No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56480.3225839615001068No Hit
GAGTAGTAGTGTAGCAGAAACCTGGGAGGGCTCCCTGGCTTCTGTTGGTA54480.3111610166877801No Hit
GTAATACACAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGAAACA53110.3033362994913363No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCAAGTATTGGCTGGCCAAATCATACAGTAATAGTCAG52280.29859577739422066No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT47740.27266569267023905No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC46580.2660403846790895No Hit
GTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTG45150.2578729791382759No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA42390.242109315297265No Hit
GTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAG42000.23988184105886132No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40550.2316002060699244No Hit
GGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCAC40460.23108617355336974No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT38980.22263319439224794No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38890.22211916187569325No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT37260.21280946185364696No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36770.2100108403746269No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAACGCAGGGATCTTACTCATCTCCGGG35590.20327130293535411No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35370.20201477900599818No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG35370.20201477900599818No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34900.1993303869751014No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCGGAGATGAGTAAGAT34460.19681733911638952No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33340.19042049002148656No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC33220.18973511333274695No Hit
CTATTACTGTATGATTTGGCCAGCCAATACTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC33060.1888212777477608No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32560.18596554154467912No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG32270.18430921454689178No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31980.1826528875491044No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31680.18093944582725538No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC30960.17682718569481776No Hit
GTGCAGGTGAGTCTGGCGGATTCTCCAGGAGATGCGGAGGAGGAAGGTGG30200.1724864666661336No Hit
GTGTATTACTGTGCAAGAGGGTATACAGCCAGTAGGCACTGGTACTTCGA29870.1706016807720997No Hit
GTGCATGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCC29170.16660365008778533No Hit
CTCCTGGAGAATCCGCCAGACTCACCTGCACCTTGCCCAGTGACATCAAT28960.16540424088249103No Hit
CTCCAGAGCCCCGGCGCTTATCTGAGTCTGAGTAGTAGTGTAGCAGAAAC28640.16357656971251874No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27580.15752240896198558No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27540.15729395006573904No Hit
TCCTCAGACTGGAGCCCGGAGATGAGTAAGATCCCTGCGTTGGCTGAAGC27190.15529493472358188No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27060.15455244331078064No Hit
CATCAATGTTGGTACCTCCAACATATACTGGTACCAACAGAAGCCAGGGAGCCCTCCCAGGTTTCTGCTACACTA27030.15438109913859574No Hit
ATGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTC26980.15409552551828756No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC26730.15266765741674673No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT26720.1526105426926851No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26090.14901231507680215No Hit
CCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCACCTGCACCTTGCCCAGTGACATCAATGTTGGTAC25190.14387198991125513No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCAAGTATTGGCTGGCCAAATCATACAGTAATAGTCAGC25110.1434150721187621No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC25020.14290103960220737No Hit
ATGTTGGTACCTCCAACATATACTGGTACCAACAGAAGCCAGGGAGCCCTCCCAGGTTTCTGCTACACTACTACT24910.1422727776375294No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24270.13861743529758483No Hit
CCCAAGTATTGGCTGGCCAAATCATACAGTAATAGTCAGCCTCATCCTCA24150.13793205860884525No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCCAGCCAATACTTGGGTATTCGGCGGAGGGACCAAG23950.13678976412761257No Hit
GTATATGTTGGAGGTACCAACATTGATGTCACTGGGCAAGGTGCAGGTGA23670.13519055185388684No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23310.13313442178766802No Hit
GAGTAAGATCCCTGCGTTGGCTGAAGCATCTTTGGATCCAGAGAAGCGGC23200.13250615982299005No Hit
GCTTGGTCCCTCCGCCGAATACCCAAGTATTGGCTGGCCAAATCATACAGTAATAGTCAGCCTCATCCTCAGA22880.13067848865301776No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCAAGTATTGG22750.1299359972402165No Hit
ATACTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC22700.12965042361990836No Hit
CTCACTGCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCG22280.12725160520931977No Hit
CTGCTACACTACTACTCAGACTCAGATAAGCGCCGGGGCTCTGGAGTCCC22050.1259379665559022No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG21780.12439586900623809No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC21680.12382472176562173No Hit
GTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTG21590.12331068924906702No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC21290.12159724752721801No Hit
CCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCAC21160.12085475611441679No Hit
GCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGA21140.12074052666629352No Hit
GTATTGGCTGGCCAAATCATACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCGGAGATGAGTAAGATCC20850.11908419966850615No Hit
CATATACTGGTACCAACAGAAGCCAGGGAGCCCTCCCAGGTTTCTGCTACACTACTACTCAGACTCAGATAAGCG20590.11759921684290366No Hit
GCCAATACTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT20290.11588577512105466No Hit
GGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGC20250.11565731622480813No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20130.11497193953606852No Hit
CCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCACCTTCCTC20110.11485771008794526No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19820.11320138309015787No Hit
ATGTTGGAGGTACCAACATTGATGTCACTGGGCAAGGTGCAGGTGAGTCT19710.1125731211254799No Hit
GATAAGCGCCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAACGCAGGGATCTTA19650.11223043278111011No Hit
GCCTCATCCTCAGACTGGAGCCCGGAGATGAGTAAGATCCCTGCGTTGGCTGAAGCATCTTTGGATCCAGAGAAG19550.11165928554049377No Hit
GACTCAGATAAGCGCCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAACGCAGGG19310.11028853216301455No Hit
TGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCC19270.11006007326676802No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19200.10966027019833659No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC19070.10891777878553537No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18960.10828951682085737No Hit
GTATCAACGCAGAGTACGGGGTGCATGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCT18800.10737568123587124No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18760.10714722233962472No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG18760.10714722233962472No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC18590.10617627203057695No Hit
GATCTTACTCATCTCCGGGCTCCAGTCTGAGGATGAGGCTGACTATTACT18570.10606204258245366No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18450.10537666589371408No Hit
GGATGAGGCTGACTATTACTGTATGATTTGGCCAGCCAATACTTGGGTATTCGGCGGAGGGACCAAGCTGACCGT18320.10463417448091283No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18090.10332053582749526No Hit
ATCTTACTCATCTCCGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTG18050.10309207693124872No Hit
GGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCC17730.10126440576127645No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC17580.10040768490035194No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17510.10000788183192051No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAA200.00752747651.45573435
GGTATCA13950.046.909021
TCGTATG1050.045.97506740
ATCGCGT306.4317527E-445.82539737
TACGTTG306.49224E-445.7384335
CGTATGC1000.044.89966241
TATACAC3750.042.7092063
CGAGACT3550.042.23203723
TTATACA3800.041.2504922
TCTCCGA3800.041.24342711
ACGAGAC3700.040.51761222
GTGGTAT3450.040.503651
TGGTATC3450.040.4967042
TCTCGTA1450.037.96493538
CACGAGA3950.037.95103521
CCCACGA4050.037.0129219
GCATCGG655.702459E-736.94257735
GTATCAA34050.036.8349461
CGTTTAA400.002259012535.49385558
CTTATAC4150.035.3142281
Produced by FastQC (version 0.11.5)