FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004688126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750862
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCA72060.411568701588132No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC71780.4099694893144063No Hit
CTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAG70600.4032299518751335No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCGGAGATGAGTAAGATCC69130.39483408743807336No Hit
CACCTGCACCTTGCCCAGTGACATCAATGTTGGTACCTCCAACATATACTGGTACCAACAGAAGCCAGGGAGCC64290.3671905609922427No Hit
CTACTACTCAGACTCAGATAAGCGCCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGC61850.35325456832120405No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG60970.3482284726037803No Hit
GACTATTACTGTATGATTTGGCCAGCCAATACTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG54470.31110390196371845No Hit
GAGTAGTAGTGTAGCAGAAACCTGGGAGGGCTCCCTGGCTTCTGTTGGTA52690.3009374810807477No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCAAGTATTGGCTGGCCAAATCATACAGTAATAGTCAG51040.2915135516105781No Hit
GTAATACACAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGAAACA46810.2673540233325071No Hit
GTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTG46150.26358445154443927No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC44950.25673068465704324No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44810.2559310785201803No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG44020.2514190153193113No Hit
GTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAG43480.2483348202199831No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43230.24690695211844224No Hit
GGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCAC42800.24445101898379196No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT40580.23177155024210933No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39340.22468932445846673No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39160.22366125942535733No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG38730.2212053262907071No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35980.20549877717375784No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35750.20418513852034026No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35360.20195766428193654No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC33510.19139144033053435No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC33450.19104875198616453No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33270.19002068695305513No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAACGCAGGGATCTTACTCATCTCCGGG33270.19002068695305513No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32550.1859084268206175No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCGGAGATGAGTAAGAT31610.18053964275882395No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC31520.18002561024226924No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31110.17768390655574226No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG30770.17574200593764672No Hit
GTGCATGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCC30210.17254358139019524No Hit
CTCCAGAGCCCCGGCGCTTATCTGAGTCTGAGTAGTAGTGTAGCAGAAAC29950.17105859856459277No Hit
GTGCAGGTGAGTCTGGCGGATTCTCCAGGAGATGCGGAGGAGGAAGGTGG29650.16934515684274373No Hit
CTCCTGGAGAATCCGCCAGACTCACCTGCACCTTGCCCAGTGACATCAAT29430.1680886329133878No Hit
CTATTACTGTATGATTTGGCCAGCCAATACTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC29390.16786017401714126No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28910.16511866726218286No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28840.16471886419375142No Hit
GTGTATTACTGTGCAAGAGGGTATACAGCCAGTAGGCACTGGTACTTCGA28540.16300542247190242No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC27250.15563762306795167No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26880.15352437827767124No Hit
TCCTCAGACTGGAGCCCGGAGATGAGTAAGATCCCTGCGTTGGCTGAAGC26260.14998326538584994No Hit
ATGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTC25990.14844116783618583No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT25680.1466706113902752No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25620.1463279230459054No Hit
CATCAATGTTGGTACCTCCAACATATACTGGTACCAACAGAAGCCAGGGAGCCCTCCCAGGTTTCTGCTACACTA25470.14547120218498089No Hit
GGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGC25400.14507139911654945No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25060.1431294984984539No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24880.14210143346534448No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24170.1380462880569685No Hit
GCTTGGTCCCTCCGCCGAATACCCAAGTATTGGCTGGCCAAATCATACAGTAATAGTCAGCCTCATCCTCAGA24160.1379891733329069No Hit
GTCTGAGGATGAGGCTGACTATTACTGTATGATTTGGCCAGCCAATACTTGGGTATTCGGCGGAGGGACCAAG23990.1370182230238591No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23950.13678976412761257No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23350.13336288068391455No Hit
CCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGACTCACCTGCACCTTGCCCAGTGACATCAATGTTGGTAC23270.13290596289142148No Hit
ATGTTGGTACCTCCAACATATACTGGTACCAACAGAAGCCAGGGAGCCCTCCCAGGTTTCTGCTACACTACTACT22540.12873658803492222No Hit
CCCAAGTATTGGCTGGCCAAATCATACAGTAATAGTCAGCCTCATCCTCA22520.12862235858679896No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCAAGTATTGG22480.12839389969055243No Hit
GTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTG22440.1281654407943059No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCAAGTATTGGCTGGCCAAATCATACAGTAATAGTCAGC22040.12588085183184056No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21950.12536681931528584No Hit
CTGCTACACTACTACTCAGACTCAGATAAGCGCCGGGGCTCTGGAGTCCC21750.12422452483405316No Hit
GTATATGTTGGAGGTACCAACATTGATGTCACTGGGCAAGGTGCAGGTGA21480.12268242728438905No Hit
CCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCAC21390.12216839476783437No Hit
GAGTAAGATCCCTGCGTTGGCTGAAGCATCTTTGGATCCAGAGAAGCGGC21370.12205416531971108No Hit
ATACTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC21140.12074052666629352No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG21030.12011226470161555No Hit
CTCACTGCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCG20890.11931265856475269No Hit
CATATACTGGTACCAACAGAAGCCAGGGAGCCCTCCCAGGTTTCTGCTACACTACTACTCAGACTCAGATAAGCG20880.11925554384069105No Hit
GCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCACCTTCCTCCTCCGCATCTCCTGGAGAATCCGCCAGA20580.11754210211884204No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC20580.11754210211884204No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACC20210.11542885732856159No Hit
CCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCCCAGCCTGTGCTGACTCAGCCACCTTCCTC20080.11468636591576034No Hit
GTATTGGCTGGCCAAATCATACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCGGAGATGAGTAAGATCC19980.11411521867514401No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19740.1127444652976648No Hit
GTATCAACGCAGAGTACGGGGTGCATGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCT19300.11023141743895294No Hit
GATAAGCGCCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCAGCCAACGCAGGGATCTTA19090.10903200823365862No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT19090.10903200823365862No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC18950.10823240209679576No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18800.10737568123587124No Hit
GACTCAGATAAGCGCCGGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATC18560.10600492785839205No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18550.10594781313433041No Hit
GCCAATACTTGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT18510.10571935423808387No Hit
TGTCAGGCTGTGTCCACCATGGCCTGGACTCCTCTTCTTCTCTTGCTCCTCTCTCACTGCACAGGTTCCCTCTCC18460.1054337806177757No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC18400.1050910922734059No Hit
GGTTTCTGCTACACTACTACTCAGACTCAGATAAGCGCCGGGGCTCTGGA18010.10286361803500219No Hit
GCCTCATCCTCAGACTGGAGCCCGGAGATGAGTAAGATCCCTGCGTTGGCTGAAGCATCTTTGGATCCAGAGAAG17870.10206401189813932No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17660.10086460269284502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)