FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739654
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGTTTACCTCCCACTTTCGGCGGAGGGA30770.4160053214070363No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21900.296084385401823No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20980.28364613724795645No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTCCCAGACCCACTGCCA17670.23889548356393667No Hit
GTATGGTAGTTTACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGGGAACTGTGGCTGCACCAT17130.23159477269101497No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17120.23145957434151646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15730.21266700376121808No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14810.20022875560735154No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTTACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGGGAA13910.1880609041524821No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13900.18792570580298357No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13160.1779210279400909No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12760.17251309396014894No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12120.16386039959224177No Hit
GTCTGGGACAGACTTCGCTCTCACCATCAGCAGACTGGAGCCTGAAGATT12020.1625084160972563No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11870.16048044085477806No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTC11830.15993964745678385No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11300.15277413493336073No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT11250.15209814318586798No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11140.15061096134138396No Hit
CTCCTGCAGGGCCAGTCAGATTCTTACCAACAACTACTTAGCCTGGTACC11070.1496645728948941No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11000.14871818444840426No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA10990.14858298609890572No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10660.14412144056545356No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTCCCAGACCCACTGCCA10600.14331025046846227No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT10300.1392542999835058No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9970.13479275445005368No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9680.13087200231459573No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9240.12492327493665957No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8570.11586498552025677No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT8280.11194423338479884No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8200.11086264658881045No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATCTCCACCTTGGTCC8130.10991625814232059No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8120.10978105979282204No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGATTCTTACCAA8010.108293877948338No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT7800.10545471260886848No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7740.10464352251187718No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7690.10396753076438443No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7530.10180435717240764No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7480.10112836542491489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACG200.00787041650.8761921
GGTATCA8750.045.3371621
GCATCGG405.2650157E-542.7495335
CGTATGC702.1722371E-839.45929341
GTATCAA21100.036.477071
TCGTATG601.3480036E-534.4770440
CACGAGA1850.033.00077421
TCTCCGA1850.032.9940811
TCTGGAG7100.032.69882670
TGCGTTC3900.032.4882652
GTGCGTT4000.031.61393451
CCCACGA1950.031.30207419
AGTGCGT4050.031.1581750
ATACACA6300.030.6768554
GCGTTCT4300.030.36343653
CGGGTAT450.005033014330.13662517
TATACAC2150.029.9634363
GACTCCT2050.029.8094126
ACAGCCG4100.029.7751468
ATGCCGT1052.2033419E-829.73983644
Produced by FastQC (version 0.11.5)