FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938959
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34940.37211422436975417No Hit
GTATAATAATTATCCGGTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT32980.35124004349497684No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32140.34229396597721523No Hit
GTAGTATATAGCCGTGTCCTCGACTCTCAGGTTGTTCATTTGCAGATAAA29660.3158817371152521No Hit
TTATTATACTGTTGGCAGTAATAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC29540.3146037260412861No Hit
GGATATTACCAAATGGGTAGGCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGTCCCTGATGTATTCTACATC23790.25336569541375076No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23700.2524071871082763No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23040.24537812620146351No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21390.22780547393443165No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20690.2203504093362969No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20370.21694237980572104No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20280.21598387150024656No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18400.19596169800811325No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTC18270.1945771860113168No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18150.19329917493735083No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17970.1913821583264019No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17850.19010414725243596No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17690.188400132487148No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16780.17870854850957285No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15980.1701884746831331No Hit
GCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT15930.16965597006898064No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15730.16752595161237072No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT15730.16752595161237072No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15630.16646094238406575No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14990.159644883322914No Hit
GTCTCCCAGGTGTCAGTTGTGACATCCAGATGACCCAGTCTCCGTCCTCA14830.15794086855762604No Hit
ATATACTACTGTGCGAGAGATTGGGGCCAGGGGACTAGTGGTTATGTCGA13840.14739727719740692No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13710.14601276520061046No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13620.14505425689513599No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13420.14292423843852606No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12890.13727968952850977No Hit
GTCTGCATCTGTAGGAGACACAGTAACCATCACTTGTCGGGCGAGTCAGG12650.13472366738057784No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12580.13397816092076437No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12510.1332326544609509No Hit
CAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTCTCCCAGGTGTCAGTTGTGACAT12500.1331261535381204No Hit
AGTCAGGATATTACCAAATGGGTAGGCTGGTATCAGCAGAAACCAGGAAA12480.13291315169245943No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12170.12961162308471405No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAATTTATTACTGCCAACAGTATAATAATTAT11900.12673609816829062No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11750.1251385843258332No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11640.12396707417469773No Hit
GCTATATACTACTGTGCGAGAGATTGGGGCCAGGGGACTAGTGGTTATGT11510.12258256217790127No Hit
GTAATATCCTGACTCGCCCGACAAGTGATGGTTACTGTGTCTCCTACAGA11440.1218370557180878No Hit
GGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCT11390.12130455110393533No Hit
GATGTATTCTACATCCATTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATATGGGACAGATTTCA11380.12119805018110481No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11290.12023954187563035No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG11130.11853552711034242No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11050.11768351972769844No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTCTCCCAGGTGTCAGTTGTGACATCCAGATGACCCAGTCTCCGTCCTCA11020.11736401695920694No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10760.11459499296561405No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10760.11459499296561405No Hit
GTAGGAGACACAGTAACCATCACTTGTCGGGCGAGTCAGGATATTACCAAATGGGTAGGCTGGTATCAGCAGAAA10600.11289097820032609No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10520.11203897081768213No Hit
CAGTAACCATCACTTGTCGGGCGAGTCAGGATATTACCAAATGGGTAGGCTGGTATCAGCAGAAACCAGGAAAAG10390.11065445882088568No Hit
GTATATAGCCGTGTCCTCGACTCTCAGGTTGTTCATTTGCAGATAAAATG10320.1099089523610722No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGG10130.10788543482729278No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10100.10756593205880129No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCGGTAACTATGGCATGCACTGGGTCCGCCAGGGTCCAGGCAAGCGG10010.10660742375332682No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9890.10532941267936086No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9870.10511641083369987No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9840.10479690806520839No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9600.10224088591727647No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9590.10213438499444598No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9580.10202788407161548No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9420.10032386930632754No Hit
GAGTGAAATCTGTCCCATATCCACTGCCGCTGAACCTTGATGGGACCCCA9390.10000436653783604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2350.054.6687544
TCGTATG2400.054.50523440
CGTATGC2450.052.1207341
ATCTCGT2600.051.37449337
CCTAACG200.00798744750.687642
TGCCGTC2600.049.5042645
ACGAGAC2700.048.8207222
TATACAC2850.048.612713
CACGAGA2650.048.4664421
CATCTCG2850.046.78713236
GCCGTCT2700.046.4894446
GTATGCC2850.046.1369842
GTGGTAT2150.045.5868641
CTTATAC3000.045.062881
TTATACA3050.044.3170622
AGACCTC3000.043.9737925
ACACATC3250.042.6296046
CTTGAAA3050.042.36295757
CTCTCTA3050.042.25425329
CGAGACC3150.041.85748323
Produced by FastQC (version 0.11.5)