FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483919
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCGCAGTTTCAGGGAGA67930.45777431247932No Hit
GTATATTACTGTGCGAGACACCGGTACCGCGTTGCTGGAGCTAATCGTGT55940.3769747540128538No Hit
GTCTCTGGTGGCTCCATCACCACTGATCCTTTTTACTGGGGCTGGATCCG32400.21834075849153492No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCAGACGTTCGGCCAAGGGA28180.1899025485892424No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT27320.18410708401199796No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25240.17009014642982534No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21940.14785173584272457No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21760.14663873162888272No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20430.13767597827105119No Hit
CCTCTGGGTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCC20310.1368673087951566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20160.13585647195028838No Hit
GGACTGGTGAGGCCGTCGGAGACCCTCTCCCTCACGTGCACTGTCTCTGG18680.12588288174758866No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18300.12332209507392249No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17050.11489845470002069No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16970.11435934171609098No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16940.11415717434711734No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16940.11415717434711734No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16450.11085510732054783No Hit
CAGTAATACACTGCAACATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA16390.11045077258260054No Hit
GCCCAGAACCAGTTCTCCCTGAAACTGCGCTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAC16170.10896821187679383No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16170.10896821187679383No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16070.10829432064688167No Hit
GGTCTGGAGTGGATTGGAAGTATGTATTATGATGGAGACAGCTTCTACAA16020.10795737503192561No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGATAATGAAGCACCTCTGGGT15630.10532919923526823No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15500.10445314063638243No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGTAGGTTAGCG15450.10411619502142637No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15200.10243146694664601No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15130.10195974308570752No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14910.1004771823799008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT19150.051.14711470
CGTATGC5800.046.42751741
TCGTATG5800.046.3477640
CGTACTA5850.045.2213729
ACCGTAC5900.044.78513727
GACCGTA5950.043.806926
GTACTAG6200.042.71187230
CTAGATC6000.041.47062733
ATCTCGT6150.041.21787637
ATGCCGT6600.041.0272844
ACGAGAC6400.040.6786822
GCCGTCT6650.040.39810646
GGTATCA14300.039.4840661
ACTAGAT6800.038.55090732
CTTATAC6950.038.431181
CCGTCTT7050.038.18437247
TACTAGA6950.038.1439931
TATGCCG7000.038.11437643
TAGATCT6700.037.17450734
GAGACCG7200.036.6420124
Produced by FastQC (version 0.11.5)