FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483919
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCGCAGTTTCAGGGAGA66250.44645293981679596No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61850.41680172570066154No Hit
GTATATTACTGTGCGAGACACCGGTACCGCGTTGCTGGAGCTAATCGTGT54540.36754027679408374No Hit
GTCTCTGGTGGCTCCATCACCACTGATCCTTTTTACTGGGGCTGGATCCG31240.21052362022455406No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCAGACGTTCGGCCAAGGGA25980.1750769415311752No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21330.1437409993402605No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20790.1401019866987349No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20370.1372716435331039No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20310.1368673087951566No Hit
CCTCTGGGTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCC19210.129454505266123No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18800.12669155122348322No Hit
GGACTGGTGAGGCCGTCGGAGACCCTCTCCCTCACGTGCACTGTCTCTGG17910.12069391927726514No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17630.11880702383351112No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGATAATGAAGCACCTCTGGGT17390.11718968488172198No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17180.11577451329890648No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16790.1131463375022491No Hit
GGTCTGGAGTGGATTGGAAGTATGTATTATGATGGAGACAGCTTCTACAA16640.11213550065738088No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16160.1089008227538026No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15660.10553136660424187No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15590.10505964274330339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT17150.054.93271670
GGTATCA17850.039.728041
GTACGGT350.001502066938.5695423
CTACGGA18050.035.6366869
GTATCAA39250.032.779431
TTCGATC450.00468294130.58613239
TCTACGG19250.029.77253368
TATCAAC44400.028.6653522
GTGTCTA19150.028.57684965
ATCAACG45000.028.198623
TCAACGC45050.028.0914654
CGCAGTT19100.028.08918435
TGTCTAC19400.028.03235866
AACGCAG45800.027.7013996
CAACGCA46150.027.4181985
CTAACGC500.00861320426.998683
GGGCCGT19600.026.98344460
GCGCTAA26700.026.71540570
GAGCGCA20050.026.67270932
AATATAC20450.026.4045773
Produced by FastQC (version 0.11.5)