FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890841
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGCTGCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCT78700.8834348666035802No Hit
GCCTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTGTTATTGTTGGAGGTGTATAAAATATGG62100.6970940942323041No Hit
ATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT59450.6673469227392991No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57970.6507334080941493No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47620.5345510590554319No Hit
ATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA39590.4444115167577604No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC38810.435655745525857No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37910.4255529325659686No Hit
ATTTTATACACCTCCAACAATAACAACTACTTAAGTTGGTACCAGCAGAA37130.4167971613340652No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35020.3931116776169934No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33730.3786309790411532No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30300.340128036316245No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT29570.3319335324710021No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29440.33047423726568487No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27790.31195241350588937No Hit
TTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT27780.3118401600285573No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26830.3011760796820083No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26770.3005025588180158No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26680.2994922775220269No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25580.2871443950154966No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25390.28501157894618684No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25110.2818684815808882No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC24730.2776028494422686No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT24720.2774905959649365No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCATATTTTATACAC23480.2635711647757568No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23480.2635711647757568No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22970.25784623743182No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGAGAA22320.2505497614052339No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22260.24987624054124136No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22100.24808018490392786No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20670.2320279376454384No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20100.22562948943750905No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGC19940.22383343380019557No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT19850.2228231525042067No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCA19480.21866977384291925No Hit
GCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT19260.21620019734161314No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18750.21047526999767638No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC18680.2096894956563517No Hit
GTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACTTA18620.20901597479235914No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18480.20744442610970984No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17970.20171949876577303No Hit
GTCAGGGACCCCGGATTCTCGGGTAGATGCCCAGTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGCTGGTA17910.20104597790178047No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17790.1996989361737953No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17500.19644358533116457No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16960.19038189755523152No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16950.19026964407789942No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16750.18802457453125754No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16640.1867897862806045No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16310.1830854215286454No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15740.17668697332071603No Hit
GTATAAAATATGGCTGGACTTGCAGTTGATGGAGGCCCTCTCGCCCAGAG15690.17612570593405558No Hit
CCTCCAACAATAACAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGG15630.17545218507006302No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15160.1701762716354546No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15080.16927824381679785No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14860.16680866731549177No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG14640.1643390908141857No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTT14620.16411458385952152No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14310.1606347260622266No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14120.1585019099929168No Hit
GTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTGTTATTGTTGGAGGTG13840.15535881262761816No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13520.15176670135299117No Hit
GTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC13340.14974613876101345No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13280.1490726178970209No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13260.14884811094235673No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13250.14873585746502463No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13240.14862360398769253No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13170.14783782964636788No Hit
GCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT12710.14267416968909155No Hit
GTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCATATTTTAT12690.14244966273442736No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12600.1414393814384385No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12530.14065360709711386No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12490.14020459318778547No Hit
CTACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGAGAATC12470.13998008623312128No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12370.13885755145980036No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12360.13874529798246826No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12320.13829628407313987No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12310.13818403059580778No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12300.1380717771184757No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12090.1357144540945017No Hit
CTACGGGGACATCGTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTG11860.13313262411586355No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGC11800.132459103251871No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11750.1318978358652105No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11650.1307753010918896No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11590.130101780227897No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11570.12987727327323284No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11500.12909149893190816No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11430.1283057245905835No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11330.12718318981726257No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11270.12650966895327No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11260.1263974154759379No Hit
CATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA11180.12549938765728116No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11070.12426459940662812No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT10920.12258079724664671No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA10880.12213178333731833No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10830.12157051595065786No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGCTGA10790.12112150204132949No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10750.1206724881320011No Hit
ATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA10360.11629460251604944No Hit
GCCATATTTTATACACCTCCAACAATAACAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAGG10310.11573333512938898No Hit
ACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATT10240.1149475607880643No Hit
TTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTTCGGCGGAGGGA10180.11427403992407174No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA10140.11382502601474337No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10090.1132637586280829No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10070.11303925167341872No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC10010.11236573080942613No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTG9970.11191671690009779No Hit
GCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCTGACCGATT9940.11157995646810148No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG9920.1113554495134373No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTA9900.11113094255877311No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG9850.11056967517211265No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9770.1096716473534559No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9680.10866136605746704No Hit
CTGCAAGTCCAGCCATATTTTATACACCTCCAACAATAACAACTACTTAA9440.10596728260149678No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA9310.10450798739617957No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9290.10428348044151536No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC9130.10248742480420187No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9070.10181390394020931No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9040.10147714350821303No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC9030.10136489003088094No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9010.10114038307621676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)