FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890841
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGGCTGCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCT67980.7630991389035754No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55420.6221087713744653No Hit
ATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT54770.6148122953478792No Hit
GCCTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTGTTATTGTTGGAGGTGTATAAAATATGG52390.5880959677428408No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC41430.4650661565868657No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38150.4282470160219388No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36660.4115212478994568No Hit
ATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA34960.39243815675300087No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34690.38940731286503427No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT32930.36965070085458573No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31300.3513533840494544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31000.3479857797294916No Hit
ATTTTATACACCTCCAACAATAACAACTACTTAAGTTGGTACCAGCAGAA29400.33002522335635653No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA27020.30330889575131814No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT26910.3020741075006651No Hit
TTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT24880.27928665160225No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24870.27917439812491796No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24380.27367397773564534No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23890.26817355734637266No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA23770.26682651561838755No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23370.2623363765251038No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23210.2605403208877903No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22580.25346835181586835No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22280.25010074749590555No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21540.24179399017333061No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21450.24078370887734174No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21070.23651807673872216No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20790.23337497937342355No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20760.23303821894142726No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20080.22540498248284488No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19940.22383343380019557No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCATATTTTATACAC18950.21272033954431824No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18790.21092428390700474No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGAGAA18540.2081179469737024No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCTCGGGT18360.20609738438172467No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT18180.204076821789747No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17790.1996989361737953No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG17310.1943107692618548No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCA16830.1889226023499143No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16820.18881034887258222No Hit
GCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT16620.18656527932594033No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16430.18443246325663054No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15920.17870753591269373No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15910.17859528243536163No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15740.17668697332071603No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGC15640.1755644385473951No Hit
GTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACTTA15560.17466641072873834No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15310.171860073795436No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15260.1712988064087755No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15220.17084979249944715No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14960.1679312020888127No Hit
GTCAGGGACCCCGGATTCTCGGGTAGATGCCCAGTAAATGAGCAGCCTAGGAGGCTGTCCTGGTTTCTGCTGGTA14830.1664719068834955No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14780.16591063949683502No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14580.16366556995019313No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14120.1585019099929168No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA13550.15210346178498743No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTA13440.15086867353433442No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13400.15041965962500603No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13330.14963388528368138No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTT13190.14806233660103205No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13150.14761332269170369No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13070.14671529487304694No Hit
GTCCTGGTTTCTGCTGGTACCAACTTAAGTAGTTGTTATTGTTGGAGGTG12950.1453682531450618No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12530.14065360709711386No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGC12520.14054135361978176No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12480.14009233971045337No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12460.13986783275578918No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12410.13930656536912872No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12340.13852079102780407No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12290.13795952364114358No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12230.13728600277715103No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12200.13694924234515474No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG12050.13526544018517334No Hit
CCTCCAACAATAACAACTACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGG12040.13515318670784124No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11970.1343674123665166No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11840.13290811716119938No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT11760.1320100893425426No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11620.13043854065989327No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG11590.130101780227897No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11020.12370333201996765No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGCTGA11000.12347882506530347No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10920.12258079724664671No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10820.12145826247332576No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10750.1206724881320011No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10700.12011122074534063No Hit
GCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGAGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT10690.11999896726800854No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10660.11966220683601225No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10560.11853967206269132No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT10460.1174171372893704No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10340.11607009556138526No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC10240.1149475607880643No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10180.11427403992407174No Hit
CTACTTAAGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGAGAATC10050.11281474471875452No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9990.11214122385476197No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9940.11157995646810148No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9910.1112431960361052No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9840.11045742169478055No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9720.10911037996679541No Hit
GTCTCTGGGCGAGAGGGCCTCCATCAACTGCAAGTCCAGCCATATTTTAT9690.10877361953479914No Hit
GTATAAAATATGGCTGGACTTGCAGTTGATGGAGGCCCTCTCGCCCAGAG9680.10866136605746704No Hit
ATTATAGTACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA9660.10843685910280286No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGC9580.10753883128414611No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTG9510.10675305694282146No Hit
TTATTACTGTCAGCAATATTATAGTACTCCGCTCACTTTCGGCGGAGGGA9490.10652854998815725No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT9470.10630404303349308No Hit
CGGGGACATCGTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGG9450.10607953607882888No Hit
GTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC9380.10529376173750422No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9250.10383446653218699No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9220.10349770610019071No Hit
CTACGGGGACATCGTGCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCTCCATCAACTG9200.10327319914552655No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8930.10024235525755999No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8920.1001301017802279No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT502.5262847E-455.37212870
TCGCGGG1500.050.75778270
GGTATCA7500.044.7222941
GCGGCAT659.212465E-442.5939470
GTGGTAT2100.042.375361
TGGTATC2150.041.3573342
GTATCAA19950.040.1450731
TATCAAC22350.035.6529962
TCAACGC23000.035.0737574
ATCAACG23000.034.9310233
CAACGCA23450.034.40075
AACGCAG23600.034.1820536
GTCACCG3500.033.6187970
GGTCTGG27000.033.0694670
ACGCAGA24900.032.3974467
CGCAGAG25350.031.9571848
AGAGTAC25850.031.46245811
GCTTGAC553.4121552E-431.1147691
CAGAGTA26400.030.81044810
GCAGAGT26600.030.710729
Produced by FastQC (version 0.11.5)