FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836713
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44870.536265123166486No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37960.4536800551682596No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28430.33978198020109646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28260.33775022020692874No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26940.3219742014286858No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26710.3192253496718708No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26130.31229346263294583No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23110.2761998439130263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22040.26341170747914755No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19960.23855252637403745No Hit
CCTCTACAGACTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAGCGAACTGTGGCTGCACCAT19900.23783543461139006No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19710.23556464402967325No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18690.22337408406466736No Hit
CTACAGACTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAGCG18510.2212228087767251No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18290.2185934723136846No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18100.21632268173196784No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG18070.21596413585064414No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17010.20329551471053994No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16840.20126375471637226No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15970.1908659241579849No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15920.19026834768911205No Hit
GTAATAAACCCCAACATCCTCAGCCTCCATTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA15620.186682888875875No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15310.1829779147688634No Hit
GGTCTAGTCAGAGCCTCCTGCATACTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC15200.18166324653734314No Hit
CCTGCATACTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAG14660.1752094206735165No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14560.1740142677357708No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14480.17305814538557426No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14400.17210202303537772No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14370.17174347715405403No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14170.16935317127856267No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA13860.16564819717155105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13630.162899345414736No Hit
GATTTTACACTGAAAATCAGCAGAATGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAACCTCTACAGACT13190.15764067248865501No Hit
GTCTGTAGAGGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCATTCTGCTGATTTTCAGTGTAAAAT13130.1569235807260076No Hit
CTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13080.15632600425713475No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13030.15572842778826193No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12990.15525036661316366No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12750.15238199956257403No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12740.15226248426879946No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC12670.15142587721237746No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12520.14963314780575895No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12490.14927460192443526No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12440.14867702545556244No Hit
GAGTTATACCAGGTCGTCAGTGCCAAATCACTACAAGTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGT12210.14592817369874736No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCGACCTTGGTCCCTTGGCCGAACGTCCACGGAGT12200.1458086584049728No Hit
GTGCAGCCACAGTTCGCTTGATATCGACCTTGGTCCCTTGGCCGAACGTC12050.14401592899835428No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12040.1438964137045797No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12010.143537867823256No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT11960.14294029135438316No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11880.14198416900418662No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11760.1405499854788918No Hit
GAGTCTGTAGAGGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCATTCTGCTGATTTTCAGTGTAAAAT11650.13923531724737157No Hit
GTTTATTACTGCATGCAACCTCTACAGACTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAGCGAA11480.1372035572532039No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT11230.1342156749088397No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11150.13325955255864316No Hit
CTGTAGAGGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCATTC11060.13218391491467205No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10920.1305107008018281No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10900.13027167021427896No Hit
GGTCGATATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC10880.13003263962672984No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10760.12859845610143503No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10700.1278813643387876No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAA10640.1271642725761402No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10510.12561057375707083No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10460.12501299728819798No Hit
CTCTACAGACTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAG10380.12405687493800145No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA10380.12405687493800145No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10220.12214463023760835No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC9910.11843965613059675No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9700.11592983496133083No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC9570.11437613614226144No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA9550.1141371055547123No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9530.11389807496716318No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9360.11186631497299553No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG9320.11138825379789724No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9300.11114922321034812No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9180.10971503968505329No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9170.10959552439127873No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG9110.10887843262863132No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9060.10828085615975848No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8970.10720521851578738No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8940.10684667263446367No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG8870.1060100655780417No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8840.10565151969671799No Hit
GGACCAAGGTCGATATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC8800.10517345852161973No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8790.10505394322784516No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8750.10457588205274689No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8670.10361975970255034No Hit
TTATTACTGCATGCAACCTCTACAGACTCCGTGGACGTTCGGCCAAGGGA8650.1033807291150012No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8600.10278315264612835No Hit
ATATCGACCTTGGTCCCTTGGCCGAACGTCCACGGAGTCTGTAGAGGTTGCATGCAGTAATAAACCCCAACATC8580.10254412205857923No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8420.10063187735818613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)