FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836713
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37750.45117023399899364No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35450.42368171643084307No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29910.35747024367973246No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28460.3401405260824201No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27470.3283085119987379No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25550.3053615755940209No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21620.25839206514061575No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20950.2503845404577197No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20490.24488683694408953No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20240.24189895459972532No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18440.22038620172030315No Hit
CCTCTACAGACTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAGCGAACTGTGGCTGCACCAT18360.21943007937010658No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18340.21919104878255746No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG18330.21907153348878292No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18250.21811541113858635No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17810.21285673821250534No Hit
CTACAGACTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAGCG16800.200785693541274No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16750.2001881170724012No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16460.1967221735529387No Hit
GTAATAAACCCCAACATCCTCAGCCTCCATTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA16080.1921805923895051No Hit
GGTCTAGTCAGAGCCTCCTGCATACTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC15350.18345597594396165No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15010.17939245595562636No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14770.17652408890503674No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14670.17532893596729104No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14600.17449232891086908No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14450.17269959950425057No Hit
CCTGCATACTGATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAG14430.17246056891670142No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13740.16421401364625623No Hit
GATTTTACACTGAAAATCAGCAGAATGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAACCTCTACAGACT13620.16277983012096142No Hit
GTCTGTAGAGGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCATTCTGCTGATTTTCAGTGTAAAAT13470.1609871007143429No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13270.15859679483885156No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13230.15811873366375329No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA13100.1565650348446839No Hit
GAGTTATACCAGGTCGTCAGTGCCAAATCACTACAAGTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGT13050.15596745837581108No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12740.15226248426879946No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12440.14867702545556244No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12410.14831847957423872No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12350.1476013878115913No Hit
CTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC12080.14437447487967797No Hit
GGTCGATATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC11950.1428207760606086No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT11800.14102804665399007No Hit
GTTTATTACTGCATGCAACCTCTACAGACTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAGCGAA11660.13935483254114614No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11630.13899628665982242No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11620.13887677136604784No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11550.13804016430962587No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCGACCTTGGTCCCTTGGCCGAACGTCCACGGAGT11540.13792064901585133No Hit
GTGCAGCCACAGTTCGCTTGATATCGACCTTGGTCCCTTGGCCGAACGTC11510.1375621031345276No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAA11280.13481325137771255No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11270.13469373608393798No Hit
GAGTCTGTAGAGGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCATTCTGCTGATTTTCAGTGTAAAAT11260.1345742207901634No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11160.13337906785241774No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11130.13302052197109404No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11090.13254246079599574No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCC11050.1320643996208975No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10960.13098876197692638No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10810.12919603257030787No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10750.12847894080766045No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10640.1271642725761402No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10540.12596911963839452No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT10420.12453493611309971No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10400.12429590552555057No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10320.12333978317535405No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10210.12202511494383379No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10080.1204714161247644No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG9900.11832014083682219No Hit
CTGTAGAGGTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCATTC9890.11820062554304762No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9840.11760304907417478No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9680.11569080437378168No Hit
CTCTACAGACTCCGTGGACGTTCGGCCAAGGGACCAAGGTCGATATCAAG9500.11353952908583946No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9460.1130614679107412No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9440.11282243732319205No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9400.1123443761480938No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9390.11222486085431921No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9220.11019310086015158No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA9220.11019310086015158No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9050.10816134086598392No Hit
TTATTACTGCATGCAACCTCTACAGACTCCGTGGACGTTCGGCCAAGGGA8820.10541248910916885No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA8810.10529297381539429No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC8780.10493442793407057No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC8590.10266363735235379No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8540.10206606088348094No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA8530.10194654558970638No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8510.10170751500215724No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8490.10146848441460812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGC150.00223742469.8991347
GACGTTA200.002905509465.439569
ACGTTAG350.004835384457.53946370
CACGTAA200.006158137754.13293561
GGTATCA10750.045.6360471
GTGGTAT2600.039.0387951
CCGGTAG350.001487111938.64629
GTATCAA25850.037.302061
AGGTATC601.5357418E-533.8275572
ATGGCAA1401.2045912E-633.56468670
TGGTATC3050.033.2730032
TCGGGTT450.00412230531.39202752
CCGATAA450.004371099631.01838546
AATTCCG450.004528966330.79428742
TCTATCG450.004912962730.2862131
TATCAAC31750.030.2583482
CTCGAAC450.00509647830.0599523
AGTCATA450.005097967630.05815312
GATTCAC2600.029.9297431
ATCAACG32400.029.5380743
Produced by FastQC (version 0.11.5)