FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297945
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT41580.3203525573117505No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37910.2920770910939986No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34080.2625689070029932No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30280.23329185751322282No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG29830.22982483849469737No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29230.2252021464699968No Hit
GATTATTACTGCAGCTCATATACAACCAGCAGCAGTTATGTCTTCGGAACTGGGACCATGATCACCGTCCTAGGT27750.213799506142402No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27550.21225860880083516No Hit
GTACTCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC25290.19484646884112963No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24770.1908401357530558No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC24440.18829765513947047No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT23960.18459950151971No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22180.17088551517976494No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21610.16649395775629938No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT21330.16433670147810578No Hit
GGTCAGTACTCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCAT21260.16379738740855737No Hit
GCCTTGGGCTGACCTAGGACGGTGATCATGGTCCCAGTTCCGAAGACATAACTGCTGCTGGTTGTATATGAGCTG21070.16233353493406885No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGATCATGGTCCCAGTTCCGAAGACATAACTGCTG21040.16210240033283382No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20760.15994514405464022No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20570.1584812915801517No Hit
GACCTAGGACGGTGATCATGGTCCCAGTTCCGAAGACATAACTGCTGCTGGTTGTATATGAGCTGCAGTAATAAT20290.1563240353019581No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20110.15493722769454793No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTCATTATAACTATGTCTCCTGGTACCAACAGCA20090.15478313796039123No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19890.15324224061882438No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG19330.14892772806243715No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTCATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC19040.1466934269171652No Hit
TTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAAC18600.1433034527657181No Hit
GTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAGTTATAATGA18440.14207073489246463No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18060.1391430299434876No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17580.13544487632372712No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17380.13390397898216028No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT16870.12997469076116475No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16450.12673880634387436No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16390.1262765371414043No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16150.12442746033152408No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16040.1235799667936623No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15780.12157680024962537No Hit
ACCTAGGACGGTGATCATGGTCCCAGTTCCGAAGACATAACTGCTGCTGG15760.12142271051546868No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15150.11672297362368975No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC14980.11541321088335793No Hit
TAGTTATAATGACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT14790.1139493584088694No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14420.11109869832697072No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14310.11025120478910895No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC13810.10639896143519179No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13540.10431875002407652No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG13390.10316307701790138No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13260.10216149374588292No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13010.10023537206892434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)