FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297945
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38010.292847539764782No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35110.2705045283120625No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34020.2621066378005231No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33660.2593330225857028No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28220.21742061489508413No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26950.20763591677613458No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG26410.20347549395390405No Hit
GATTATTACTGCAGCTCATATACAACCAGCAGCAGTTATGTCTTCGGAACTGGGACCATGATCACCGTCCTAGGT26340.20293617988435564No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24020.18506177072218005No Hit
GTACTCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC23210.17882113648883427No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22630.17435253419829036No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22050.16988393190774648No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT21310.1641826117439491No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20990.1617171759974421No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20460.1576337980422899No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT20420.15732561857397653No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGATCATGGTCCCAGTTCCGAAGACATAACTGCTG20290.1563240353019581No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20160.15532245202993963No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19940.1536274649542161No Hit
GCCTTGGGCTGACCTAGGACGGTGATCATGGTCCCAGTTCCGAAGACATAACTGCTGCTGGTTGTATATGAGCTG19830.15277997141635433No Hit
GGTCAGTACTCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCAT19660.15147020867602248No Hit
GACCTAGGACGGTGATCATGGTCCCAGTTCCGAAGACATAACTGCTGCTGGTTGTATATGAGCTGCAGTAATAAT19570.1507768048723174No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18910.14569184364514676No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTCATTATAACTATGTCTCCTGGTACCAACAGCA18420.14191664515830793No Hit
TTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAAC18080.13929711967764427No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17920.13806440180439078No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTCATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC17780.13698577366529396No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG17740.1366775941969806No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17220.13267126110890676No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17070.13151558810273165No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT16710.12874197288791128No Hit
GTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACATAGTTATAATGA16400.12635358200848262No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16280.1254290436035425No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16150.12442746033152408No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15290.11780160176278656No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15010.11564434548459294No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14850.11441162761133947No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14450.11132983292820574No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13780.10616782683395676No Hit
ACCTAGGACGGTGATCATGGTCCCAGTTCCGAAGACATAACTGCTGCTGG13740.10585964736564339No Hit
TAGTTATAATGACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAT13680.10539737816317332No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC13420.1033942116191364No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC13200.10169922454341285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)