FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832549
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21420.2572821539633103No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19810.23794395284842093No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATTACAATCTGACTGCCTGCATATGAGCTGCAGTAATAAT19530.23458078743713584No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19420.2332595438827024No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19080.22917570016899907No Hit
GTCCTGGGCCCAGTCTGCCCTGGCTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG18930.2273740044129535No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17260.2073151249956459No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC17190.20647433364282464No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16530.19854687231622403No Hit
CTCCTGCACTGGAGGCAGCGGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAGCAACACCCAGGCAAAGC16000.19218088064486297No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15860.19049929793922038No Hit
GATTATTACTGCAGCTCATATGCAGGCAGTCAGATTGTAATCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT15700.18857748913277175No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15180.18233161051181374No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAGGCAGCGGTGACGTT15170.1822114974614107No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14950.17956901035254383No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCAGCTCATAT14880.17872821899972255No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATTACAATCTGACTGCCTGCATATGAGCTG14440.1734432447819888No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13690.16443476600176085No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13690.16443476600176085No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13440.16143193974168488No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13410.16107160059047576No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12550.15074187825581437No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12470.14978097385259004No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12450.14954074775178397No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12450.14954074775178397No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12420.14918040860057485No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12350.14833961724775357No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG12300.1477390519957384No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12070.14497645183646848No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11780.14149317337478035No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11550.13873057321551044No Hit
GTCAGTCACCATCTCCTGCACTGGAGGCAGCGGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAGCAACA11470.13776966881228614No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11230.13488695560261318No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11150.13392605119938886No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11110.13344559899777672No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11040.13260480764495544No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11010.13224446849374633No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10920.13116345104011895No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGGCTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC10920.13116345104011895No Hit
GCTTATAACTATGTCTCCTGGTACCAGCAACACCCAGGCAAAGCCCCCAAACTCATGCTTTATGAGGTCAGTAAA10170.12215497225989101No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATTACAATCTGACTGC10150.12191474615908494No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10080.12107395480626365No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACT9740.11699011109256033No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9690.11638954584054513No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC9520.11434762398369344No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9380.1126660412780509No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGGCT9170.11014366721958707No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8960.10762129316112323No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8930.10726095400991414No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8780.10545925825386855No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8770.1053391452034655No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8640.10377767554822599No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8600.10329722334661384No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8600.10329722334661384No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGGCTCAGCCTCCCTCCGCGTCCGGGT8580.10305699724580775No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8570.10293688419540471No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8550.10269665809459864No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8530.10245643199379255No Hit
GTCTCAGGTGGCTCCGTCAGTAGTGGTTATTACTACTGGAGCTGGGTCCG8500.10209609284258345No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAGGCAGCGGTGACGTTGGTG8490.1019759797921804No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC8470.10173575369137433No Hit
GCCCTGGCTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC8410.10101507538895609No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGGCTCAGC8410.10101507538895609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAC200.00612723254.20176757
ATTCGCG200.00796496650.7232630
TGCGACG200.008156938550.4185816
TCCGAGA6400.040.8822570
GTACGTA557.684072E-540.09364769
GTATCAA17000.035.9828641
GGATGGG5700.035.03114370
TACGTAT800.002621397134.4271670
GGTATCA6850.034.346361
TTAACGG400.002755287334.08326335
GTGAGCG2106.548362E-1032.78776670
CTGAGCG951.0686563E-832.03574430
TGTGTCG9900.031.99291470
TATCAAC19100.031.4987222
TAACGGC450.00486856530.34243636
ATCAACG20000.030.081283
AATGGTA5050.029.99593770
AGGGGGG1154.2741644E-429.93665970
CTACGGG450.005251833229.8758813
TAGTACA450.005251833229.8758814
Produced by FastQC (version 0.11.5)