FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832549
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20210.24274847486454249No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19570.23506123963874798No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATTACAATCTGACTGCCTGCATATGAGCTGCAGTAATAAT18060.21692416902788905No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17990.21608337767506777No Hit
GATTATTACTGCAGCTCATATGCAGGCAGTCAGATTGTAATCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT16410.19710551571138754No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16180.19434291555211763No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16000.19218088064486297No Hit
CTCCTGCACTGGAGGCAGCGGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAGCAACACCCAGGCAAAGC15980.19194065454405687No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC15940.1914602023424447No Hit
GTCCTGGGCCCAGTCTGCCCTGGCTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG15670.18821714998156264No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15560.18689590642712922No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAGGCAGCGGTGACGTT15090.18125059305818636No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14670.1762058449412587No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14350.17236222732836146No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATTACAATCTGACTGCCTGCATATGAGCTG13950.16755770531223987No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13930.1673174792114338No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13470.161792278892894No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13280.15951013093523625No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCAGCTCATAT13180.15830900043120585No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13060.15686764382636936No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12870.15458549586871165No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12470.14978097385259004No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12390.14882006944936577No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12380.14869995639896272No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12340.14821950419735055No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11910.14305464303001986No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11520.13837023406430132No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11490.1380098949130922No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11350.13632831220744965No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11340.13620819915704663No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11080.13308525984656758No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10910.13104333798971593No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10850.13032265968729767No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGGCTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC10830.1300824335864916No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10640.12780028562883386No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG10190.12239519836069708No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9940.1193923721006211No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9920.11915214599981504No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9880.11867169379820286No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9790.11759067634457551No Hit
GCTTATAACTATGTCTCCTGGTACCAGCAACACCCAGGCAAAGCCCCCAAACTCATGCTTTATGAGGTCAGTAAA9770.11735045024376944No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9630.11566886753812688No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9580.11506830228611169No Hit
GTCAGTCACCATCTCCTGCACTGGAGGCAGCGGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAGCAACA9480.11386717178208129No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACT9410.11302638042926003No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9400.11290626737885698No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9340.11218558907643876No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9270.11134479772361747No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9260.11122468467321443No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9130.10966321501797492No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8940.10738106706031716No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC8870.1065402757074959No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATTACAATCTGACTGC8780.10545925825386855No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8420.10113518843935913No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC8360.10041451013694089No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA252.587597E-455.10960840
GTATAGG252.9358247E-453.7187841
ACGACCG250.00671631152.9571969
TCTAGTG605.4518605E-447.37916670
GGAATAT658.0875313E-443.73461570
CGCTACA359.6422347E-442.20508263
GGTATCA7950.040.120161
GGATGGG7000.039.59544870
GTGGTAT2700.036.0612221
TGTGTCG8600.033.05523370
GTATCAA18250.032.5624161
AATGGTA5500.032.30397870
TGGTATC3250.029.9495532
CCCGGTT450.005199345829.93731327
TAGCCTG450.005288828629.8330145
TAACCCA450.00529193629.829434
TATCAAC20050.028.9605942
ATAGGTG4900.028.7640934
GTCGATG500.00665345528.46370552
TAAGTCA2253.4082404E-828.42749870
Produced by FastQC (version 0.11.5)