FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964751
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42600.44156471462584646No Hit
GTAATAATCGGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG41640.43161396049343304No Hit
GATTATTACTGTGCAACATGGGATCACAGCCTGAGTGGTTATGTGATATTCGGCGGAGGGACCCAGCTGACCGT40830.4232180116942092No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31510.32661277365869534No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29500.3057783821939547No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28590.2963458965059378No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAAGACGGTCAGCTGGGTCCCTC26650.2762370808633523No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26310.27271285544145585No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25460.2639022918867148No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25120.26037806646481837No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24730.25633557259852546No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24420.2531223082432669No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC23380.24234232459981903No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23240.24089117295550874No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22240.23052580406757806No Hit
GCCTTGGGCTGACGTAAGACGGTCAGCTGGGTCCCTCCGCCGAATATCAC21460.22244081633499216No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21390.221715240512837No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21220.21995312780188878No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTGGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG20540.21290467695809592No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20150.208862183091803No Hit
GTCTTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19160.19860046789275163No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT18870.1955945109152517No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18830.19517989615973447No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18690.19372874451542418No Hit
CCCCTGAGGGCCGCAGATAATTCCTATAGATGATGAGTTTGGGGGCCGCTCCTGGGAGTTGTTGGTACCAGTATA18590.1926922076266311No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18040.18699125473826927No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17990.18647298629387274No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17620.18263779980533837No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17210.1783879985612868No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16580.17185781616189047No Hit
GGCCATCAGTGGGCTCCGGTCCGAAGATGAGGCCGATTATTACTGTGCAA16370.16968108869542503No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT16300.1689555128732699No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16300.1689555128732699No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16270.16864455180663196No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16170.1676080149178389No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15460.16024860300740812No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC15210.15765726078542547No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14930.15475495749680487No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14850.15392572798577042No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAAGATG14650.15185265420818428No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14560.15091977100827053No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14240.14760285296413272No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14180.14698093083085687No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14080.1459443939420638No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14020.14532247180878796No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAAGACGGTCAGC13990.14501151074215005No Hit
ACATGGGATCACAGCCTGAGTGGTTATGTGATATTCGGCGGAGGGACCCA13960.14470054967551213No Hit
GTGCAACATGGGATCACAGCCTGAGTGGTTATGTGATATTCGGCGGAGGGACCCAGCTGACCGTCTTACGTCAGC13490.13982882629818472No Hit
GAGTACGGGAGCTTCAGCTGTGGTGGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT13370.13858498203163302No Hit
GTTATGTGATATTCGGCGGAGGGACCCAGCTGACCGTCTTACGTCAGCCC13370.13858498203163302No Hit
GACGTAAGACGGTCAGCTGGGTCCCTCCGCCGAATATCACATAACCACTC13320.1380667135872365No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13260.13744479145396066No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC13230.13713383038732274No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13110.13588998612077105No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12990.13464614185421936No Hit
GTGGTGGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12850.13319499020990908No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12590.1304999942990471No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12520.12977441847689195No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12480.12935980372137473No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12190.12635384674387484No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAAGACGGTCAGCTGGGTCCCTCCGCCGAATATCACATAACCA12050.12490269509956456No Hit
ATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCCCTGCCCGGGGGT11750.12179308443318534No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC11690.12117116229990951No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11670.1209638549221509No Hit
GAACAAGAGATGGTGACCCCCTGCCCGGGGGTCCCAGACGCTGAGGGTGG11620.12044558647775437No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11580.12003097172223715No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11510.11930539590008199No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT11230.1164030926114614No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11160.11567751678930625No Hit
TCACATAACCACTCAGGCTGTGATCCCATGTTGCACAGTAATAATCGGCC11140.11547020941154763No Hit
GTTTGGGGGCCGCTCCTGGGAGTTGTTGGTACCAGTATACATAATTAGTC11120.11526290203378903No Hit
GTACGGGAGCTTCAGCTGTGGTGGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC11090.1149519409671511No Hit
CTCCTGGGAGTTGTTGGTACCAGTATACATAATTAGTCCCAATATTGGAGCTGCTTCCAGAACAAGAGATGGTGA11040.11443367252275458No Hit
TCTTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10910.1130861745673236No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10780.1117386766118926No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG10600.10987291021206508No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10510.10894002701215132No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCACCATCTCTTGTTCTGG10420.10800714381223755No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCAG10410.10790349012335826No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10270.10645233847904796No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10230.10603772372353074No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10220.10593407003465143No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG10180.1055194552791342No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG10150.10520849421249628No Hit
GTCCGAAGATGAGGCCGATTATTACTGTGCAACATGGGATCACAGCCTGA10130.10500118683473766No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10100.10469022576809976No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10090.10458657207922044No Hit
GTATACATAATTAGTCCCAATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCCCTGCCCGGGGGTCCCAGA9710.10064773190180679No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9680.10033677083516887No Hit
ATGTTGCACAGTAATAATCGGCCTCATCTTCGGACCGGAGCCCACTGATG9650.10002580976853094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT250.001112992883.34086670
CGCGTAT252.602101E-455.04828339
AGGGGGG2300.054.35273770
GGTATCA8450.046.3260841
TGTCGTA306.435161E-445.8183638
ATCGCGT306.4817566E-445.7513337
AATCGCG306.523585E-445.69164736
GTGGTAT2150.044.3306431
GTATCAA22550.039.851241
TGGTATC2150.039.580932
GTCGTAT350.001366060239.320239
TCGCGTA350.001374171139.2728838
CTAATCG350.001405557239.0928734
AAAACCG1253.8029975E-738.89240370
TATACAC3600.038.7673263
TACACAT3600.037.821785
TCGTATG2750.037.58411440
CGAGACT3450.037.49872623
GTATAAG1001.0913936E-1137.443561
ACGAGAC3550.037.3956222
Produced by FastQC (version 0.11.5)