FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964751
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39400.4083955341844683No Hit
GATTATTACTGTGCAACATGGGATCACAGCCTGAGTGGTTATGTGATATTCGGCGGAGGGACCCAGCTGACCGT37660.39035979231946893No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31620.32775296423636774No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31460.32609450521429884No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27220.28214534112947276No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26300.2726092017525766No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC25660.26597536566430097No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25240.2616219107313701No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25230.26151825704249076No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24310.25198211766559453No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23360.2421350172220604No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23220.2406838655777501No Hit
GTAATAATCGGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG23050.2389217528668019No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAAGACGGTCAGCTGGGTCCCTC22020.22824542291223332No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20080.2081366072696478No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTGGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG20070.2080329535807685No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19580.20295392282568248No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19550.20264296175904456No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19380.20088084904809636No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT19110.1980821994483551No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18970.19663104780404478No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18460.19134470967120012No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC17750.18398529776076936No Hit
CCCCTGAGGGCCGCAGATAATTCCTATAGATGATGAGTTTGGGGGCCGCTCCTGGGAGTTGTTGGTACCAGTATA17690.18336337562749352No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17670.1831560682497349No Hit
GCCTTGGGCTGACGTAAGACGGTCAGCTGGGTCCCTCCGCCGAATATCAC17660.1830524145608556No Hit
GTCTTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17630.18274145349421766No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17140.17766242273913166No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16710.17320531411732148No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16640.1724797382951663No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16560.17165050878413185No Hit
GGCCATCAGTGGGCTCCGGTCCGAAGATGAGGCCGATTATTACTGTGCAA15980.16563859482913207No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15920.16501667269585624No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15820.16398013580706317No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT15710.1628399452293908No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15340.15900475874085646No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15030.15579149438559795No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14450.14977958043059816No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAAGATG14400.14926131198620163No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14160.14677362345309827No Hit
GTGGTGGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13920.1442859349199949No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13490.13982882629818472No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13250.13734113776508136No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13230.13713383038732274No Hit
GTGCAACATGGGATCACAGCCTGAGTGGTTATGTGATATTCGGCGGAGGGACCCAGCTGACCGTCTTACGTCAGC13170.1365119082540469No Hit
GTTATGTGATATTCGGCGGAGGGACCCAGCTGACCGTCTTACGTCAGCCC13170.1365119082540469No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12970.13443883447646077No Hit
ACATGGGATCACAGCCTGAGTGGTTATGTGATATTCGGCGGAGGGACCCA12930.13402421972094353No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12710.1317438385655988No Hit
GAGTACGGGAGCTTCAGCTGTGGTGGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT12470.1292561500324954No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12460.1291524963436161No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12440.12894518896585752No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12380.1283232668325817No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12310.12759769101042653No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11850.12282962132197842No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAAGACGGTCAGC11650.12075654754439227No Hit
CTCCTGGGAGTTGTTGGTACCAGTATACATAATTAGTCCCAATATTGGAGCTGCTTCCAGAACAAGAGATGGTGA11580.12003097172223715No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11580.12003097172223715No Hit
GTTTGGGGGCCGCTCCTGGGAGTTGTTGGTACCAGTATACATAATTAGTC11570.11992731803335782No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11490.11909808852232338No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11340.11754328318913379No Hit
ATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCCCTGCCCGGGGGT11340.11754328318913379No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC11240.11650674630034072No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAAGACGGTCAGCTGGGTCCCTCCGCCGAATATCACATAACCA11010.11412271145611666No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10950.1135007893228408No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10910.1130861745673236No Hit
GACGTAAGACGGTCAGCTGGGTCCCTCCGCCGAATATCACATAACCACTC10910.1130861745673236No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT10840.11236059874516843No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCACCATCTCTTGTTCTGG10690.11080579341197884No Hit
GTACGGGAGCTTCAGCTGTGGTGGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC10510.10894002701215132No Hit
GAACAAGAGATGGTGACCCCCTGCCCGGGGGTCCCAGACGCTGAGGGTGG10280.10655599216792726No Hit
CTCTTGTTCTGGAAGCAGCTCCAATATTGGGACTAATTATGTATACTGGTACCAACAACTCCCAGGAGCGGCCCC10260.10634868479016865No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10160.1053121479013756No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10150.10520849421249628No Hit
TCTTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10100.10469022576809976No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10020.1038609962570653No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA9880.102409844612755No Hit
GTCCGAAGATGAGGCCGATTATTACTGTGCAACATGGGATCACAGCCTGA9680.10033677083516887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTG150.00249269268.0213620
TACGTAT400.006330718853.75240370
TCGGTTA200.00771939551.1273429
GGTATCA9150.045.7548521
GTGGTAT2400.045.3828621
TGGTATC2500.040.821282
GACAGGT1400.038.8995971
GTATCAA21800.037.6282231
TAAGTCT601.036397E-535.8271161
TATCAAC24600.033.0497472
GACAGCC3000.032.9025761
ATCAACG25700.031.3623093
GACAGCT1650.030.942861
TCAACGC26050.030.9361214
CGGGTCA553.543282E-430.91559418
CAACGCA27550.029.251765
AACGCAG27600.029.1987676
GATTGAC704.2220134E-529.1746981
ACGCAGA28000.028.7816417
CGCAGAG28700.028.3166128
Produced by FastQC (version 0.11.5)