FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826851
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC42630.51557051996067No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31780.3843497800692023No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAGCCACCCTCAGCGTCTGGGGCCC30480.3686274794370449No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24660.298239948914617No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23860.28856468698713555No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22400.2709073339694818No Hit
GCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGGCGAATATGCG21790.26352994674977714No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21680.2621995982347484No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTC19570.23668109490101605No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19270.23305287167821048No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19170.23184346393727528No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19060.23051311542224656No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18960.2293037076813114No Hit
GTAGTAGATTCCGGAGTCCCCAGGTCTCACATCGTTCATTTGTAAGTACA18650.22555454368441233No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17790.21515363711236973No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17320.20946942072997432No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17130.20717154602219748No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17090.2066877829258234No Hit
ATCTACTACTGTGCAAAGGGATCTACCACTCCCTATGGGGACCTGGTCAA16900.20438990821804656No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16870.204027085895766No Hit
GTCTGGGGGCCGTTCCTGGGACATGTTGATACCAATAGAGAAAACTCTTC16490.19943133648021227No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16370.19798004719109005No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16280.19689158022424838No Hit
GGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG15890.19217489003460114No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15220.1840718581703354No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTCCTCACTCAATGTGCAGAGTCCTGGGCCCAGT15120.18286245042940022No Hit
GGTCATCAGTGACCTCCAGTCTGAAGATGAAGCTTATTATTATTGTACAATTTGGGATGATAGTCTGAATGGTCG14710.17790387869156596No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14690.1776619971433789No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14510.1754850632096956No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14100.1705264914718613No Hit
CCCTCAGCGTCTGGGGCCCCCGGACAGACGATCACCATCTCTTGTTTTGG13780.1666563867008687No Hit
CTGATGACCAGGGAGGCTGAGGAGGCAGACCTGGAGCCAGAGAACCGCTC13760.1664145051526817No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG13630.16484227508946594No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13460.1627862819298761No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13380.16181875573712798No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13200.15964182180344463No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13170.15927899948116409No Hit
GTTCTCTGGCTCCAGGTCTGCCTCCTCAGCCTCCCTGGTCATCAGTGACC13060.15794865096613536No Hit
GCCCCCAGACTCCTCATCGATAATTTTGATCGGCGGCCCGCAGGGGTCCCTGAGCGGTTCTCTGGCTCCAGGT12850.1554088947101715No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12680.15335290155058168No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTCCTCACTCAATGTGCAGAGTCCTGGGC12590.15226443458374No Hit
GACGATCACCATCTCTTGTTTTGGCGGCAGATCCGACATCAAGAAGAGTT12540.1516597307132724No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT12390.14984561910186964No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12360.14948279677958906No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTCCTCACTCAATGTGCAGAGT12340.149240915231402No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12210.1476686851681863No Hit
CCCCTGCGGGCCGCCGATCAAAATTATCGATGAGGAGTCTGGGGGCCGTT12070.14597551433087702No Hit
CCTTGGTCCCTCCGGCGAATATGCGACCATTCAGACTATCATCCCAAATTGTACAATAATAATAAGCTTCATCTT11940.14440328426766127No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11870.14355669884900665No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11830.14307293575263258No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11480.13884000865935942No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11410.1379934232407048No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11410.1379934232407048No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC11210.13557460775883443No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11060.13376049614743166No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTCCTCA10750.13001133215053257No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTC10580.12795533899094275No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10480.12674593125000755No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10270.12420617499404366No Hit
TCTTCAGACTGGAGGTCACTGATGACCAGGGAGGCTGAGGAGGCAGACCTGGAGCCAGAGAACCGCTCAGGGACC10210.12348053034948255No Hit
AGTCTGACCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGACAGACG10140.12263394493082792No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGG10080.12190830028626681No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10080.12190830028626681No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10020.1211826556417057No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9740.11779631396708719No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9520.11513561693702976No Hit
TGTTGATACCAATAGAGAAAACTCTTCTTGATGTCGGATCTGCCGCCAAA9450.11428903151837515No Hit
ATGTGAGACCTGGGGACTCCGGAATCTACTACTGTGCAAAGGGATCTACCACTCCCTATGGGGACCTGGTCAA9320.11271680145515939No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTC9320.11271680145515939No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9300.11247491990697235No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9290.11235397913287883No Hit
GTCTGTAGAGGGTCGATTCGCCGTCTCCAGAGACAACACCCGAAATTTGT9220.11150739371422422No Hit
CTCCAGGTCTGCCTCCTCAGCCTCCCTGGTCATCAGTGACCTCCAGTCTG9210.11138645294013069No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT9120.11029798597328902No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG9110.11017704519919551No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC9040.10933045978054087No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG9020.10908857823235384No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8980.10860481513597976No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8980.10860481513597976No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC8920.10787917049141864No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8860.10715352584685753No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTCCTCACTCAATGTGCAGAGTCCTGGGCCCAGTCTGAC8810.10654882197638994No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8750.10582317733182883No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8740.10570223655773531No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8720.10546035500954827No Hit
CCGCAGGGGTCCCTGAGCGGTTCTCTGGCTCCAGGTCTGCCTCCTCAGCCTCCCTGGTCATCAGTGACCTCCAGT8700.10521847346136123No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8670.10485565113908069No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA8580.10376718417223901No Hit
GAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTG8530.10316248030177143No Hit
GTATCAACATGTCCCAGGAACGGCCCCCAGACTCCTCATCGATAATTTTG8520.1030415395276779No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT8460.10231589488311679No Hit
CCTCCAGTCTGAAGATGAAGCTTATTATTATTGTACAATTTGGGATGATA8400.10159025023855567No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8400.10159025023855567No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8370.10122742791627512No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8360.10110648714218161No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8340.10086460559399456No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8300.1003808424976205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)