FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826851
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39360.47602288683208943No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAGCCACCCTCAGCGTCTGGGGCCC29710.359315039831844No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28990.3506073040971106No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26020.3146878941913356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24880.3009006459446744No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23250.2811872997674309No Hit
GCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGGCGAATATGCG22940.27743813577053184No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22650.27393085332181977No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22550.27272144558088457No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20250.2449050675393753No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20100.24309095592797253No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18970.2294246484554049No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTC18950.22918276690721784No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18620.22519172136213178No Hit
GTCTGGGGGCCGTTCCTGGGACATGTTGATACCAATAGAGAAAACTCTTC18360.22204726123570026No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17440.21092071001909654No Hit
GTAGTAGATTCCGGAGTCCCCAGGTCTCACATCGTTCATTTGTAAGTACA16120.1949565278387521No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16100.19471464629056506No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15870.19193300848641412No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15780.19084454151957245No Hit
ATCTACTACTGTGCAAAGGGATCTACCACTCCCTATGGGGACCTGGTCAA15710.18999795610091783No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15320.1852812659112706No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15300.18503938436308356No Hit
GGTCATCAGTGACCTCCAGTCTGAAGATGAAGCTTATTATTATTGTACAATTTGGGATGATAGTCTGAATGGTCG15120.18286245042940022No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14830.17935516798068818No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14690.1776619971433789No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14630.1769363524988178No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14610.1766944709506308No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG14500.17536412243560207No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTCCTCACTCAATGTGCAGAGTCCTGGGCCCAGT14350.1735500108241993No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14340.17342907005010577No Hit
GGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG14250.1723406030832641No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13840.1673820313454298No Hit
CTGATGACCAGGGAGGCTGAGGAGGCAGACCTGGAGCCAGAGAACCGCTC13690.16556791973402704No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13530.16363286734853075No Hit
GTTCTCTGGCTCCAGGTCTGCCTCCTCAGCCTCCCTGGTCATCAGTGACC13500.1632700450262502No Hit
CCTTGGTCCCTCCGGCGAATATGCGACCATTCAGACTATCATCCCAAATTGTACAATAATAATAAGCTTCATCTT13310.16097217031847336No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGG13180.15939994025525758No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13170.15927899948116409No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT12610.15250631613192703No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC12120.14658021820134462No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11890.14379858039719368No Hit
CCCTCAGCGTCTGGGGCCCCCGGACAGACGATCACCATCTCTTGTTTTGG11670.14113788336713629No Hit
GCCCCCAGACTCCTCATCGATAATTTTGATCGGCGGCCCGCAGGGGTCCCTGAGCGGTTCTCTGGCTCCAGGT11560.13980753485210756No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC11560.13980753485210756No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTCCTCACTCAATGTGCAGAGT11480.13884000865935942No Hit
GACGATCACCATCTCTTGTTTTGGCGGCAGATCCGACATCAAGAAGAGTT11310.1367840154997696No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11290.13654213395158257No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTCCTCACTCAATGTGCAGAGTCCTGGGC11190.13533272621064737No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11030.13339767382515108No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTCCTCA11000.13303485150287053No Hit
CCCCTGCGGGCCGCCGATCAAAATTATCGATGAGGAGTCTGGGGGCCGTT10810.1307369767950937No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10720.12964850982825202No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10650.12880192440959737No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10480.12674593125000755No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10330.12493181963860478No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10300.12456899731632422No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCATCCTC10250.12396429344585663No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10060.12166641873807979No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9940.12021512944895756No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9870.11936854403030292No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9740.11779631396708719No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9650.11670784700024552No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9580.11586126158159088No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9560.11561938003340384No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9370.113321505325627No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC9240.11174927526241124No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9220.11150739371422422No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9210.11138645294013069No Hit
CTCCAGGTCTGCCTCCTCAGCCTCCCTGGTCATCAGTGACCTCCAGTCTG9210.11138645294013069No Hit
TCTTCAGACTGGAGGTCACTGATGACCAGGGAGGCTGAGGAGGCAGACCTGGAGCCAGAGAACCGCTCAGGGACC9050.1094514005546344No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTC9000.10884669668416679No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC8970.10848387436188624No Hit
TGTTGATACCAATAGAGAAAACTCTTCTTGATGTCGGATCTGCCGCCAAA8960.10836293358779273No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8900.10763728894323162No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCACCTTGGTCCCTCCGGCGAATATG8860.10715352584685753No Hit
GTCTGAATGGTCGCATATTCGCCGGAGGGACCAAGGTGACCGTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCA8850.10703258507276403No Hit
AGTCTGACCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGACAGACG8820.10666976275048347No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8780.10618599965410938No Hit
GACTGGAGGTCACTGATGACCAGGGAGGCTGAGGAGGCAGACCTGGAGCC8600.10400906572042605No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8530.10316248030177143No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8480.10255777643130382No Hit
GTCTGTAGAGGGTCGATTCGCCGTCTCCAGAGACAACACCCGAAATTTGT8400.10159025023855567No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8360.10110648714218161No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8360.10110648714218161No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8310.100501783271714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA252.7113565E-454.59092717
AATACTT200.00769561851.166616
GTATTAA350.001422708538.9958761
TTCAATA350.001424825238.9840854
GTGGTAT2000.035.827461
GCTTGTA400.002740236234.121391
GATTGAC905.7771103E-934.121391
ACAGGGG1450.032.9447941
AATGGTA3400.031.11468370
TATTAGA553.478808E-431.0119462
ATTAATC553.481276E-431.0081943
ATCAACG15600.027.7682323
CAACGCA15750.027.505445
TCAACGC15750.027.505444
TAACGGC500.00792335427.46326436
CGGGTAC500.00816387627.29546418
GACAGGT907.520832E-626.538861
ACGCAGA16500.026.4619227
TGCACAA3006.002665E-1126.4474870
AATACGT659.244019E-426.24087510
Produced by FastQC (version 0.11.5)