FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835365
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27100.32440909063702694No Hit
GTATGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25770.30848790648399205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24660.29520030166454186No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23820.2851448169363093No Hit
GCCTTGGGCTGACCTAGTACGGTCAGCTTGGTCCCTCCACCGAAGACCACATTCAGGCTGGTGGCATAGGACTGG20470.24504258617490557No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18120.2169111705661597No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG17990.21535496459631417No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16860.20182794347381083No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT16580.19847611523106665No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16430.19668049295816797No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16300.19512428698832246No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGTACGGTCAGCTTGGTCCCTC16160.19344837286695038No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGATAACGACATTCGG15710.18806150604825436No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14790.17704835610780914No Hit
GATTATTACTGCCAGTCCTATGCCACCAGCCTGAATGTGGTCTTCGGTGGAGGGACCAAGCTGACCGTACTAGGT14660.17549215013796363No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14590.17465419307727761No Hit
GTCCTATGCCACCAGCCTGAATGTGGTCTTCGGTGGAGGGACCAAGCTGACCGTACTAGGTCAGCCCAAGGCTGC14320.17142207298606No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14240.17046440777384736No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT14210.17010528331926764No Hit
GTACTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14170.1696264507131613No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13830.16555637356125766No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGCCACCAGCCTGAATGTGGT12960.15514176437844535No Hit
GTAATACACGGCCGTGTCCTCGGCCCTCAGGCTGTTCATGTGAAGATACA12740.15250818504486063No Hit
CTCCTGCATTGGGAGCAGCTCCGACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC12660.151550519832648No Hit
CCCTCCATACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12330.14760015083227093No Hit
GACCTAGTACGGTCAGCTTGGTCCCTCCACCGAAGACCACATTCAGGCTGGTGGCATAGGACTGGCAGTAATAAT12140.14532569595326594No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11910.14257240846815464No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTATGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11760.14077678619525597No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11740.1405373698922028No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11560.1383826231647244No Hit
GGGCAGAGGGTCACCATCTCCTGCATTGGGAGCAGCTCCGACATCGGGGCAGGTTATGATGTACACTGGTACCAG11560.1383826231647244No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTATGGAGGGTGTGGTGGTCTCCA11440.13694612534640546No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11410.13658700089182574No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGCCACCAGCCTGAATGTGGTCTTCGGTGGAGGGACCAAGCTGAC11120.13311546449755496No Hit
GCCTGAATGTGGTCTTCGGTGGAGGGACCAAGCTGACCGTACTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT10880.1302424688609171No Hit
GTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGGCAGAGAATCGGTC10690.1279680139819121No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC10490.12557385095138054No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10450.12509501834527423No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC10440.12497531019374764No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10330.12365852052695528No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10100.12090523304184397No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT9980.11946873522352505No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT9650.11551836622314797No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9640.11539865807162139No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT9630.11527894992009481No Hit
TACTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9590.11480011731398848No Hit
CCACCACACCCTCCATACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9220.11037091570750511No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGTACGGTCAGC9090.1088147097376596No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9000.10773733637392038No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8920.10677967116170775No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC8730.10450521628270278No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8660.10366725922201672No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCATTGGGAGCAG8540.10223076140369779No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG8420.10079426358537884No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8390.10043513913079911No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8390.10043513913079911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)