FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495389
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCGAGGACGGTCAGGTGGGTGCCACCTCCGAAAAGAGCACCACTCAGGCCGTTGTCATAGGT62720.41942263852415657No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC60760.4063156810702767No Hit
CTCCTGTACTGGGACCAGGTCCAACATCGGGGCAAATTTTGATGTACACTGGTACCAGCAATTCCCACGAAGAGC51570.3448600999472378No Hit
GTCCTGGGCCCAGGCTGTGCTGACACAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC32560.21773598709098435No Hit
GTAATATACGCCCGTGTCTTCGGCTCTCAGGCTGTTCAGTTGCAGATAAA30220.20208788482461754No Hit
GTCATAGGTCTGGCAGTAATAATCAGCCTCATCGTCAGGCTGGAGTCCAG29480.1971393396634588No Hit
GGCACATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGGTTTATCTG28800.19259202789374535No Hit
GTGCTGACACAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC28710.19199017780657743No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28280.18911467183455274No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT27880.18643978255825075No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG27660.1849685934562846No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGGTGGGTGCCAC26870.17968568713558813No Hit
TCACTGGACTCCAGCCTGACGATGAGGCTGATTATTACTGCCAGACCTAT26150.1748708864382445No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25470.17032357466853107No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25460.1702567024366235No Hit
ACCTATGACAACGGCCTGAGTGGTGCTCTTTTCGGAGGTGGCACCCACCTGACCGTCCTCGGTCAGCCCAAGG24410.1632351180863307No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24250.1621651623758099No Hit
GTACAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG23540.15741723391037382No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22630.15133186080678673No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT22520.15059626625580366No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21950.1467845490370733No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21850.1461158267179978No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21600.14444402092030903No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21370.14290595958643537No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21220.14190287610782212No Hit
GGCCCAGGCTGTGCTGACACAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC20910.13982983691868806No Hit
GTACTGGGACCAGGTCCAACATCGGGGCAAATTTTGATGTACACTGGTAC20640.1380242866571842No Hit
GATTATTACTGCCAGACCTATGACAACGGCCTGAGTGGTGCTCTTTTCGG20480.13695433094666337No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20370.13621873639568033No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19940.13334323042365565No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19580.13093583007498383No Hit
GTCCAGTGATGGCCAGGGAGGCTGACGTGTCAGACTTGGAAGCAGAGAAT19490.13033397998781587No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG19390.12966525766874037No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC18850.12605415714573265No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18510.12378050126087593No Hit
GTATGTGCCAGCCCCACTTCCACTCATACCTGAGACCCACTCCAGCCCCTTCCCTGGGGCCTGGCGGACCCAGCT18290.12230931215890982No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18210.12177433430364942No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18190.12164058983983432No Hit
GTCCAACATCGGGGCAAATTTTGATGTACACTGGTACCAGCAATTCCCACGAAGAGCCCCCAAACTCCTCATCT18020.12050376189740597No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGG17900.11970129511451537No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17640.11796261708491905No Hit
GTAATAATCAGCCTCATCGTCAGGCTGGAGTCCAGTGATGGCCAGGGAGG17530.11722702253393598No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA17320.11582270566387744No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17310.11575583343196988No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17200.11502023888098681No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17110.11441838879381887No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17010.11374966647474336No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC16920.11314781638757541No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTACTTATTATGCCATGAGCTGGGTCCGCCAGGCCCCAGGGAAGGGG16860.1127465829961301No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16830.11254596630040745No Hit
CTGCACAGGGTCCTGGGCCCAGGCTGTGCTGACACAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC16780.1122116051408697No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16680.1115428828217942No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16640.111275393894164No Hit
ATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGGTTTATCTGCAA16560.11074041603890358No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16450.11000482148792053No Hit
GTATATTACTGCGCGAAGAATGGGGCGGTTGCTGCTATCTTCTACTACGG16360.10940297140075259No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16310.10906861024121482No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC16220.10846676015404688No Hit
GGGCAGAGGGTCACCATCTCCTGTACTGGGACCAGGTCCAACATCGGGGCAAATTTTGATGTACACTGGTACCAG16220.10846676015404688No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16060.10739680444352606No Hit
CTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGGTTTATCTGCAACTGAA15960.10672808212445056No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC15570.1041200650800561No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15510.10371883168861079No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC15380.10284949267381263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)