FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495389
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCGAGGACGGTCAGGTGGGTGCCACCTCCGAAAAGAGCACCACTCAGGCCGTTGTCATAGGT59370.39702044083512716No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC58480.3910688121953552No Hit
CTCCTGTACTGGGACCAGGTCCAACATCGGGGCAAATTTTGATGTACACTGGTACCAGCAATTCCCACGAAGAGC52820.35321912893568164No Hit
GTCCTGGGCCCAGGCTGTGCTGACACAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC31850.2129880586255483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29940.20021546233120613No Hit
GTCATAGGTCTGGCAGTAATAATCAGCCTCATCGTCAGGCTGGAGTCCAG29370.19640374511247574No Hit
GTGCTGACACAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC29230.19546753386577004No Hit
GTAATATACGCCCGTGTCTTCGGCTCTCAGGCTGTTCAGTTGCAGATAAA27540.18416612667339402No Hit
GGCACATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGGTTTATCTG26100.17453652527870672No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT25720.17199538046621982No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25420.16998921350899332No Hit
TCACTGGACTCCAGCCTGACGATGAGGCTGATTATTACTGCCAGACCTAT25320.16932049118991782No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGGTGGGTGCCAC25100.1678493020879517No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24980.1670468353050611No Hit
GTACAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG24800.16584313513072518No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT24560.16423820156494398No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23650.1581528284613569No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23450.15681538382320587No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23150.15480921686597937No Hit
ACCTATGACAACGGCCTGAGTGGTGCTCTTTTCGGAGGTGGCACCCACCTGACCGTCCTCGGTCAGCCCAAGG22290.14905820492193No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT22240.14872384376239225No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21290.14237098173117496No Hit
GATTATTACTGCCAGACCTATGACAACGGCCTGAGTGGTGCTCTTTTCGG20500.1370880754104785No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20290.13568375854041992No Hit
GTACTGGGACCAGGTCCAACATCGGGGCAAATTTTGATGTACACTGGTAC20280.13561688630851235No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19960.13347697488747073No Hit
GTCCAGTGATGGCCAGGGAGGCTGACGTGTCAGACTTGGAAGCAGAGAAT19740.13200578578550465No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19600.13106957453879894No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19500.13040085221972345No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19490.13033397998781587No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG19030.12725785732006856No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18830.12592041268191756No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18730.12525169036284206No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTACTTATTATGCCATGAGCTGGGTCCGCCAGGCCCCAGGGAAGGGG18720.1251848181309345No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18700.1250510736671194No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18570.12418173465232124No Hit
GGCCCAGGCTGTGCTGACACAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC18540.1239811179565986No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18050.1207043785931286No Hit
GTATGTGCCAGCCCCACTTCCACTCATACCTGAGACCCACTCCAGCCCCTTCCCTGGGGCCTGGCGGACCCAGCT17420.11649142798295294No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC17290.11562208896815478No Hit
GTCCAACATCGGGGCAAATTTTGATGTACACTGGTACCAGCAATTCCCACGAAGAGCCCCCAAACTCCTCATCT17250.11535460004052459No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17190.11495336664907928No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGG17160.11475274995335662No Hit
GTAATAATCAGCCTCATCGTCAGGCTGGAGTCCAGTGATGGCCAGGGAGG16960.1134153053152056No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC16880.1128803274599452No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG16800.11234534960468481No Hit
CTGCACAGGGTCCTGGGCCCAGGCTGTGCTGACACAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC16460.11007169371982808No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16310.10906861024121482No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16270.10880112131358463No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG16220.10846676015404688No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA16180.10819927122641666No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC16170.10813239899450913No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16010.10706244328398831No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15960.10672808212445056No Hit
ATGTTGGACCTGGTCCCAGTACAGGAGATGGTGACCCTCTGCCCTGGGGC15710.1050562763267618No Hit
GGGTGAGGGTCGGGGGCCTAAGAGCATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15520.10378570392051835No Hit
CTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGGTTTATCTGCAACTGAA15480.10351821499288813No Hit
GGGCAGAGGGTCACCATCTCCTGTACTGGGACCAGGTCCAACATCGGGGCAAATTTTGATGTACACTGGTACCAG15370.10278262044190509No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15310.10238138705045978No Hit
GTATATTACTGCGCGAAGAATGGGGCGGTTGCTGCTATCTTCTACTACGG15290.10224764258664468No Hit
ATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGGTTTATCTGCAA15080.10084332571658614No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC15070.10077645348467858No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15040.10057583678895592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)