FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011047
Sequences flagged as poor quality0
Sequence length21-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATACACAGCCGTGTCCTCGGGTCTCAAGTCACTCATTTGAAGGTA42660.42193884161666073No Hit
GTCTAGAGACAGTTCCAAGAACACGCTGTACCTTCAAATGAGTGACTTGA42580.42114758265441665No Hit
GTATTATTGTGTGCGAGACCATATAGCAGTGCAGATTATTCCACCGAGAA34410.340340261135239No Hit
GTCCTGGACCCAGTCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCCCCTG32880.3252074334823208No Hit
GATTATTACTGCTCCTTATATAGAATTGACGGGTCTCCCCACGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTC27660.27357778619589396No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25810.2552799226939994No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG23750.23490500441621406No Hit
GCTATATGGTCTCGCACACAATAATACACAGCCGTGTCCTCGGGTCTCAA22970.22719022953433418No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22680.2243219157961994No Hit
GTACCAACACCACCCAGGCAAGGCCCCCAGAGTCATCATTTATGGGGTCATGACTCGGCCCTCAGAGGTTTCTG22130.21888201043077127No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTC21610.2137388271761847No Hit
GTCTCTAGACAGGGTGAATCGGCCCTTTACGGAGTCTGCGTAGTATGTGGTACCGTCACTATGAATGACCGAGAC21430.2119584945111355No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19850.1963311300068147No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19100.18891307723577638No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19090.18881416986549587No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18670.1846600603137144No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18060.17862671072660322No Hit
GTCGATCACCATCCCCTGCACTACTGACGTTGGTGATTTTAAATATGTCG18020.17823108124548115No Hit
ACCACATACTACGCAGACTCCGTAAAGGGCCGATTCACCCTGTCTAGAGACAGTTCCAAGAACACGCTGTACCTT17750.17556058224790738No Hit
CCATATAGCAGTGCAGATTATTCCACCGAGAAAATACAACTACTTTTACT17730.17536276750734636No Hit
GTCTGGGGGAGGCTCGGTCCGGCCGGGGGGGTCCCTGAGACTCTCCTGCGCAGCCTCTGGAATCGTCGTCAGTTC17140.1695272326607962No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16830.16646110418210033No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16770.16586765996041727No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16360.1618124577789163No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15920.15746053348657382No Hit
GTGTTGGTACCAGACGACATATTTAAAATCACCAACGTCAGTAGTGCAGGGGATGGTGATCGACTGTCCAGGAGA15800.1562736450432077No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15520.15350423867535337No Hit
GACGTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACGTGGGGAGAC15140.14974575860469394No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14870.14707525960712015No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14760.1459872785340345No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14750.14588837116375403No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14470.1431189647958997No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14090.13936048472524026No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14090.13936048472524026No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14070.13916266998467924No Hit
ATAATACACAGCCGTGTCCTCGGGTCTCAAGTCACTCATTTGAAGGTACAGCGTGTTCTTGGAACTGTCTCTAGA13930.13777796680075208No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13770.13619544887626392No Hit
CTCCTGCGCAGCCTCTGGAATCGTCGTCAGTTCCAACTACATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG13570.1342173014706537No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13210.13065663614055528No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13120.1297664698080307No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13100.12956865506746967No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12980.12838176662410353No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12950.128085044513262No Hit
GCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCAC12700.12561236025624922No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12670.12531563814540767No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCTCCTTATAT12450.12313967599923645No Hit
CCCCTGCACTACTGACGTTGGTGATTTTAAATATGTCGTCTGGTACCAACACCACCCAGGCAAGGCCCCCAGAGT12430.1229418612586754No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12410.12274404651811438No Hit
GGGCACAGGGTCCTGGACCCAGTCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12360.12224950966671184No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12330.1219527875558703No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12220.12086480648278468No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12190.12056808437194313No Hit
ATATAGCAGTGCAGATTATTCCACCGAGAAAATACAACTACTTTTACTACGGTATGGACGTCTGGGGCCGGGGGA12110.11977682540969906No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGACCCAGTCTGCCCTGACTCAGC11980.1184910295960524No Hit
CTCCTGGACAGTCGATCACCATCCCCTGCACTACTGACGTTGGTGATTTT11820.11690851167156424No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11720.11591943796875911No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11460.11334784634146583No Hit
GAACACAGAGGACTCGCCATGGAGCTTGGGCTGAGCTGGGTTTTCCTTGT11350.11225986526838021No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11330.11206205052781917No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGCTTGGGCTGAGCTGGG11290.11166642104669713No Hit
CCTTGGTCCCTCCGCCGAAAACCACGTGGGGAGACCCGTCAATTCTATATAAGGAGCAGTAATAATCAGCCTCGT11270.11146860630613611No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11240.11117188419529457No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11190.11067734734389201No Hit
CTGCTATATGGTCTCGCACACAATAATACACAGCCGTGTCCTCGGGTCTC11160.11038062523305049No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11150.11028171786276997No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCCCGG11100.10978718101136743No Hit
GTATGTGGTACCGTCACTATGAATGACCGAGACCCACTCCAGCCCCTTCC11060.10939155153024537No Hit
CTACTGACGTTGGTGATTTTAAATATGTCGTCTGGTACCAACACCACCCA10740.10622651568126902No Hit
GCACTGAACACAGAGGACTCGCCATGGAGCTTGGGCTGAGCTGGGTTTTC10570.10454509038650033No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10540.1042483682756588No Hit
ATATAGAATTGACGGGTCTCCCCACGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTACGTCAGCCCAAGG10500.10385273879453674No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10490.10375383142425625No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10450.1033582019431342No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10400.10286366509173164No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10380.10266585035117062No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10370.1025669429808901No Hit
GTGTATTATTGTGTGCGAGACCATATAGCAGTGCAGATTATTCCACCGAG10370.1025669429808901No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10340.10227022087004858No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10260.10147896190780448No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10250.10138005453752398No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCACC10200.10088551768612142No Hit
ATATAAGGAGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGCTGCCAGA10200.10088551768612142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7100.047.9143261
CGTATGC1400.044.38447641
ATGCCGT1350.043.66411244
CGGTATG11200.043.09088570
GTGGTAT1600.042.5239641
TCTCGTA1250.041.24537338
GCCGTCT1450.038.35428246
ACGAGAC1600.038.26399222
TCGTATG1550.037.80319640
ACGGGAC451.08838634E-437.7953383
GTATTAT14200.037.76352770
CACGAGA1650.037.10264621
TCTCTAG14200.036.8194470
ATCTCGT1400.036.79312537
GTATGCC1600.036.7526442
CGTGCAA754.5565685E-836.27993811
TGGTATC1900.035.806112
GTATCAA17050.035.5156141
TATACAC1650.035.046593
AATGGTA2750.034.124570
Produced by FastQC (version 0.11.5)