FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011047
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAGACAGTTCCAAGAACACGCTGTACCTTCAAATGAGTGACTTGA42720.42253228583834385No Hit
CAATAATACACAGCCGTGTCCTCGGGTCTCAAGTCACTCATTTGAAGGTA39510.3907830199782998No Hit
GTCCTGGACCCAGTCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCCCCTG37210.3680343248137821No Hit
GTATTATTGTGTGCGAGACCATATAGCAGTGCAGATTATTCCACCGAGAA34130.3375708547673847No Hit
GATTATTACTGCTCCTTATATAGAATTGACGGGTCTCCCCACGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTC26560.2626979754650377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25320.25043346155025437No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24090.23826785500575148No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23340.2308498022347131No Hit
GCTATATGGTCTCGCACACAATAATACACAGCCGTGTCCTCGGGTCTCAA21690.21453008613842878No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG20940.20711203336739045No Hit
GTACCAACACCACCCAGGCAAGGCCCCCAGAGTCATCATTTATGGGGTCATGACTCGGCCCTCAGAGGTTTCTG20590.20365027540757255No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19300.1908912246413866No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18810.18604476349764154No Hit
GTCTCTAGACAGGGTGAATCGGCCCTTTACGGAGTCTGCGTAGTATGTGGTACCGTCACTATGAATGACCGAGAC18780.1857480413868No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTC18510.18307754238922622No Hit
GTCTGGGGGAGGCTCGGTCCGGCCGGGGGGGTCCCTGAGACTCTCCTGCGCAGCCTCTGGAATCGTCGTCAGTTC18450.18248409816754316No Hit
CCATATAGCAGTGCAGATTATTCCACCGAGAAAATACAACTACTTTTACT18060.17862671072660322No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17820.17625293383987095No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17610.17417587906398022No Hit
GTCGATCACCATCCCCTGCACTACTGACGTTGGTGATTTTAAATATGTCG17350.1716042874366869No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16670.16487858625761218No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16270.16092229144639172No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15930.15755944085685433No Hit
ACCACATACTACGCAGACTCCGTAAAGGGCCGATTCACCCTGTCTAGAGACAGTTCCAAGAACACGCTGTACCTT15750.15577910819180513No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15610.15439440500787796No Hit
GTGTTGGTACCAGACGACATATTTAAAATCACCAACGTCAGTAGTGCAGGGGATGGTGATCGACTGTCCAGGAGA15480.15310860919423133No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15350.15182281338058468No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14890.14727307434768117No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14730.145690556423193No Hit
GGGCACAGGGTCCTGGACCCAGTCTGCCCTGACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14640.1448003900906684No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14470.1431189647958997No Hit
CTCCTGCGCAGCCTCTGGAATCGTCGTCAGTTCCAACTACATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG14340.14183316898225307No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14120.13965720683608182No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13850.136986707838508No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13790.13639326361682494No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCTCCTTATAT13730.13579981939514188No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13130.1298653771783112No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12670.12531563814540767No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12610.1247221939237246No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12570.12432656444260257No Hit
ATAATACACAGCCGTGTCCTCGGGTCTCAAGTCACTCATTTGAAGGTACAGCGTGTTCTTGGAACTGTCTCTAGA12480.12343639811007796No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12370.12234841703699234No Hit
CCCCTGCACTACTGACGTTGGTGATTTTAAATATGTCGTCTGGTACCAACACCACCCAGGCAAGGCCCCCAGAGT12330.1219527875558703No Hit
ATATAGCAGTGCAGATTATTCCACCGAGAAAATACAACTACTTTTACTACGGTATGGACGTCTGGGGCCGGGGGA12300.12165606544502876No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12200.12066699174222366No Hit
GACGTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACGTGGGGAGAC12140.12007354752054059No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11990.11858993696633292No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11980.1184910295960524No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGACCCAGTCTGCCCTGACTCAGC11820.11690851167156424No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11530.11404019793342941No Hit
CTGCTATATGGTCTCGCACACAATAATACACAGCCGTGTCCTCGGGTCTC11510.1138423831928684No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11400.11275440211978277No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11390.11265549474950225No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11350.11225986526838021No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11350.11225986526838021No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11260.1113696989358556No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11160.11038062523305049No Hit
GGGCTGGAGTGGGTCTCGGTCATTCATAGTGACGGTACCACATACTACGCAGACTCCGTAAAGGGCCGATTCACC11110.10988608838164793No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGCTTGGGCTGAGCTGGG10940.10820466308687925No Hit
GACTCAGCCTGCCGCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCC10920.10800684834631823No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10880.10761121886519619No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGACCCAGTC10650.10533634934874442No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10530.10414946090537829No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10380.10266585035117062No Hit
GAACACAGAGGACTCGCCATGGAGCTTGGGCTGAGCTGGGTTTTCCTTGT10370.1025669429808901No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10360.1024680356106096No Hit
ATATAGAATTGACGGGTCTCCCCACGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTACGTCAGCCCAAGG10330.10217131349976806No Hit
GTGTATTATTGTGTGCGAGACCATATAGCAGTGCAGATTATTCCACCGAG10260.10147896190780448No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10240.10128114716724346No Hit
CCTTGGTCCCTCCGCCGAAAACCACGTGGGGAGACCCGTCAATTCTATATAAGGAGCAGTAATAATCAGCCTCGT10200.10088551768612142No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10130.10019316609415783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC150.002501167.963854
CTAGTAC252.7648816E-454.376463
ACGGTTC200.007742360751.08911527
GCGAACG200.007803227350.98801818
GGTATCA8150.044.6518481
TCTCTAG14200.044.18149670
CGGTATG12650.039.89166370
CTAGTCA350.001299409739.7224846
TGGTATC2650.038.492952
GTATTAT15450.037.51728470
GTGGTAT2750.037.1023861
GTATCAA20050.036.639761
TCTAGTA400.002787981934.00212
ACGGCTG10100.032.45522362
ATACACC450.003965276331.64275658
CGGCTGT10400.031.6035163
TGTATTA12100.031.2684269
TATCAAC23450.031.0296382
TGTGTAT10500.030.73602767
CGTGTTC12950.030.26336154
Produced by FastQC (version 0.11.5)