FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112569
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCGGCTCTCAGGCTGTTCAATTGCAGATACA43090.3873018212802981No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25380.22812068285202985No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25260.22704209806313136No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24300.21841341975194348No Hit
GTGTATTACTGTGCGAGAGATTTTTGGCTTGGTAGTGGCTGGGGCCAGGG20740.18641540434795506No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19550.17571943852471172No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18690.16798958087093924No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18480.16610205749036688No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17580.15801267157362825No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCCTA16660.14974352152540651No Hit
CAGTAATACACAGCCGTGTCGTCGGCTCTCAGGCTGTTCAATTGCAGATA16280.14632800302722795No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATCTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA15750.14156425354292634No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15740.14147437147718478No Hit
GTTTATTACTGCATGCAAGCTGCACACTTTCCCGTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15610.14030590462254477No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15430.13868802743919703No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15380.13823861711048932No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15340.13787908884752317No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15120.13590168340120926No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15040.13518262687527696No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14890.13383439588915383No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14230.12790217955021216No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14180.12745276922150447No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14160.12727300509002137No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTCAGTGGCTGTGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG13440.12080149635663047No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13150.1181949164501258No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTATTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGATAGAGT12960.11648715720103651No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12960.11648715720103651No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATGAGATTTCTAAGCGGCTC12440.11181328978247641No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12310.11064482292783638No Hit
GAATTAGGACTCCTCAGGTCACCTTCTCACAATGAGGCTCCTTGCTCAGCTTCTGGGGCTGCTAATGCTCTGGGT12200.10965612020467945No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11740.10552154518056857No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11710.10525189898334394No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC11600.104263196260187No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11490.10327449353703007No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11410.10255543701109773No Hit
GTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATTTCACACTGAA11280.10138697015645771No Hit
GTCTGGGACCCCAGAGAGCCGCTTAGAAATCTCATAAATTAGGAGTCTTGGAGGCTGGCCTGGCCTCTGCTGAAA11260.10120720602497463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTG200.007684841351.1859219
CTAGCGC2050.046.71751428
GGTATCA12100.045.970691
TCGTATG1800.044.1726840
ACTAGCG2200.043.51066627
TCTGGAG15100.042.06585370
CGTATGC1850.041.14389841
GTGGTAT2500.040.950581
CGCTCAT1950.040.4286732
TAGCGCT2400.039.92793329
CGAGACT2500.039.60336323
ACGAGAC2550.038.81635322
GACTAGC2500.038.27387626
CTTATAC2600.038.0630381
ATCTCGT1750.035.42053237
TGGTATC2900.035.3022232
TATCAAC33400.034.8406872
AGACTAG2850.034.75855325
CACGAGA2900.034.12701821
GCCGTCT1950.033.90429346
Produced by FastQC (version 0.11.5)