FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112569
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCGGCTCTCAGGCTGTTCAATTGCAGATACA34140.30685737244161937No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25600.23009808829834374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24450.2197616507380666No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24210.21760448116026962No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19220.1727533303552409No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19200.1725735662237578No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18130.16295618518941296No Hit
GTGTATTACTGTGCGAGAGATTTTTGGCTTGGTAGTGGCTGGGGCCAGGG17570.1579227895078867No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17500.15729361504769593No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15320.13769932471604007No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCCTA15060.13536239100676004No Hit
GTAATAAACCCCGACATCCTCAGCTTCCACCCTGCTGATCTTCAGTGTGAAATCTGTCCCTGCCCCACTGCCA15060.13536239100676004No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15040.13518262687527696No Hit
GTTTATTACTGCATGCAAGCTGCACACTTTCCCGTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA14960.13446357034934464No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14310.12862123607614448No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13940.12529559964370748No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13940.12529559964370748No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTATTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGATAGAGT13480.12116102461959663No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13310.11963302950199044No Hit
CAGTAATACACAGCCGTGTCGTCGGCTCTCAGGCTGTTCAATTGCAGATA13090.11765562405567655No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13020.11702644959548575No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12930.11621751100381189No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12870.11567821860936264No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12860.11558833654362112No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12660.1137906952287903No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTCAGTGGCTGTGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG12180.10947635607319636No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11670.1048923707203778No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC11500.1033643756027716No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11360.10210602668239002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11230.10093755982775No Hit
GGCCAGGCCAGCCTCCAAGACTCCTAATTTATGAGATTTCTAAGCGGCTC11150.10021850330181768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG200.007158409352.11285844
GGTATCA10550.040.7177161
TCTGGAG12950.036.8291370
TATCAAC30750.034.679392
GTTCGGG601.4561512E-534.0930061
GTGGTAT3100.034.0930061
ATCAACG32100.033.0939333
TCAACGC32400.032.7875064
CAACGCA32950.032.238775
GTATCAA35250.032.110291
AACGCAG33450.031.858666
TGGTATC3450.030.6136282
CGCAGAG34950.030.5887558
AGAGTAC35600.030.41006711
CAGAGTA35700.029.94478610
ACGCAGA35700.029.8507617
GCAGAGT36100.029.6143199
GATAGTC500.00713049728.06381452
TCGAGAC500.0082536127.23518211
ATTGCAG12350.025.16678239
Produced by FastQC (version 0.11.5)