FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572871
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60920.38731720528892705No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49350.3137574537263386No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAA42120.26779055625032183No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA41610.26454807800512564No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38820.2468098146637582No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38430.24433027247625522No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37300.23714595793297732No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35070.22296806286084492No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32200.2047211754810153No Hit
GCGTTACAACTGGCCTCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT31540.20052502716370255No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30750.19550236478388883No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAACTGGTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCT30050.19105190444734502No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTTACAAC29380.1867921781252245No Hit
CACTTCACTCTCACCATCACCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTTACAACTGG28630.18202382776464185No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28530.18138804771656417No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28450.18087942367810203No Hit
GTTGTAACGCTGGTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGGTGATGGTGAGAGTGAAGTGTGT28230.17948070757233112No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG27860.17712832139444365No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27330.1737586871396319No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27000.17166061298097554No Hit
GTCTGGGACACACTTCACTCTCACCATCACCAGCCTAGAGCCTGAAGATT26130.1661293265626997No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26040.16555712451942975No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24600.15640189182711106No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24470.15557537776461008No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24300.15449455168287798No Hit
GTTTATTACTGTCACCAGCGTTACAACTGGCCTCCGGTCACCTTCGGCCA24270.1543038176684547No Hit
CACCATCACCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTTACAACTGGCCTCCGGTCAC24050.15290510156268378No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23350.14845464122613997No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22760.1447035389424816No Hit
CAATAATACATGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGAAA22640.1439406028847884No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22640.1439406028847884No Hit
GTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAACTGGTTAGCCTGGTT22240.14139748269247765No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22090.14044381262036112No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22060.1402530786059378No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACACACTTCACT21800.13860005048093582No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT21470.13650197632227945No Hit
GTATTATTGTGCTAGAGTAACGGCAGTGGCAATTGACTACTGGGGCCAGG21200.1347853701924697No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG20610.13103426790881134No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20440.12995344182707927No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20050.12747389963957628No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC19770.12569371550495875No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19550.12429499939918785No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC19490.12391353137034124No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19420.12346848533668688No Hit
GAGTGAAGTGTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA19260.12245123725976256No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACACACTTCA19180.1219426132213004No Hit
GTTAGTAACTGGTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAG19000.12079820913476057No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18960.1205438971155295No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT18890.12009885108187512No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGGTGATGGTGAGAGTGAAGTGTGTCCCAGACCCACTGCCA18800.1195266490386052No Hit
GAGTGTTAGTAACTGGTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC18790.11946307103379743No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18740.11914518100975859No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18680.11876371298091197No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18630.11844582295687314No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18320.1164749048078323No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18260.11609343677898569No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18150.11539407872610023No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA18030.11463114266840702No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17820.11329600456744388No Hit
GTCAGAGTGTTAGTAACTGGTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG17690.11246949050494287No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC17690.11246949050494287No Hit
GTCGTGTCCCTTGGCCGAAGGTGACCGGAGGCCAGTTGTAACGCTGGTGA17570.11170655444724964No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17460.1110071963943642No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC17460.1110071963943642No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17380.11049857235590203No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16860.10719251610589807No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16750.10649315805301263No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAACAGC16620.10566664399051162No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCA16160.10274205576935426No Hit
GTCTCTGGATTCACCTTCAGTGCCAACTACATGGACTGGGTCCGCCAGGC16080.10223343173089211No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACACACTTCACTCTCACCATCACCAG15780.10032609158665905No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15750.10013535757223574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1350.046.04722241
TCGTATG1450.045.19491640
GACGCAA650.001172617940.5597470
GGTATCA9850.037.6190531
GTATCAA25050.037.184371
TGCGTTA400.002421224734.9954549
CGAGACA1750.034.98094623
CCCACGA1800.033.9973619
TGTTAGA15350.033.4915170
ATCTCCG1950.033.1214210
ATACGGA550.004084368731.45272369
TATCAAC29450.031.397942
GCCGTCT2000.031.34101546
ATCAACG30150.030.6689683
CGTTTAG450.00469899130.56487538
TCAACGC30150.030.5562154
CAACGCA30450.030.0318815
ACATGCG2050.029.89411427
CTTATAC2050.029.8494431
AACGCAG31150.029.4661456
Produced by FastQC (version 0.11.5)