FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572871
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60800.38655426923123387No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42330.269125694351285No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41110.2613691777647372No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAA40700.2587624795676187No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38680.24591972259644942No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36930.2347935717550899No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35620.2264648531252722No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35000.22252301682719056No Hit
GCGTTACAACTGGCCTCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT31550.20058860516851035No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30920.19658319086562087No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAACTGGTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCT30850.1961381448319665No Hit
GTTGTAACGCTGGTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGGTGATGGTGAGAGTGAAGTGTGT29400.18691933413484005No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTTACAAC28390.18049795564925541No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28240.1795442855771389No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28070.1784634594954068No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG27870.17719189939925142No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27660.17585676129828828No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27550.17515740324540283No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26790.1703254748800124No Hit
CACTTCACTCTCACCATCACCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTTACAACTGG26150.1662564825723152No Hit
GTCTTTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAACTGGTTAGCCTGGTT25820.16415840841365886No Hit
GTCTGGGACACACTTCACTCTCACCATCACCAGCCTAGAGCCTGAAGATT25440.16174244423096362No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24280.15436739567326246No Hit
GTTTATTACTGTCACCAGCGTTACAACTGGCCTCCGGTCACCTTCGGCCA23980.15246005552902941No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23680.15055271538479634No Hit
CACCATCACCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCACCAGCGTTACAACTGGCCTCCGGTCAC23090.14680161310113798No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22970.14603867704344475No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22550.14336840084151847No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22260.14152463870209317No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21390.1359933522838173No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21200.1347853701924697No Hit
CAATAATACATGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGAAA21190.13472179218766192No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20960.13325949807708326No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20910.1329416080530444No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20830.13243298401458226No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20750.13192435997612012No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20260.12880903774053942No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACACACTTCACT20240.1286818817309239No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19920.1266473855770753No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19780.12575729350976653No Hit
GAGTGAAGTGTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA19640.12486720144245778No Hit
GTATTATTGTGCTAGAGTAACGGCAGTGGCAATTGACTACTGGGGCCAGG19640.12486720144245778No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19480.12384995336553346No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT19010.12086178713956834No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC18880.12003527307706736No Hit
GAGTGTTAGTAACTGGTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC18770.1193359150241819No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18730.11908160300495081No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG18670.1187001349761042No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18530.11781004290879545No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT18160.11545765673090799No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18080.11494903269244586No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGGTGATGGTGAGAGTGAAGTGTGTCCCAGACCCACTGCCA18010.11450398665879147No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17930.11399536262032932No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17910.11386820661071378No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC17870.1136138945914827No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17740.11278738052898171No Hit
GTTAGTAACTGGTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAG17570.11170655444724964No Hit
GTCGTGTCCCTTGGCCGAAGGTGACCGGAGGCCAGTTGTAACGCTGGTGA17510.11132508641840304No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17340.11024426033667097No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACACACTTCA17330.11018068233186319No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAACAGC17150.10903627824532336No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16870.10725609411070583No Hit
GTCAGAGTGTTAGTAACTGGTTAGCCTGGTTCCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG16710.10623884603378154No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16410.10433150588954848No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA16340.10388645985589409No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16120.10248774375012318No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16020.1018519637020455No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15730.10000820156262019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)