FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151779
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33030.2867737647586907No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31390.2725349220640418No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26650.23138119378804442No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26140.22695326099885482No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAT26080.22643232772953842No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25200.21879197311289755No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24630.2138431070543915No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG20620.17902740022174393No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20320.1764227338751618No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19260.1672195794505717No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19030.16522266858485873No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCATGCTGCCGGTTGTGTATGAGGTGCAGTAATAGTCAC18640.16183660233430197No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18370.15949240262237807No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18320.15905829156461437No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18080.1569745584873487No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18080.1569745584873487No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17470.15167840358263174No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACATT17440.15141793694797354No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16980.14742411521654764No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15650.13587676108003358No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15600.1354426500222699No Hit
CTCCTGCACTGGAACCAGCAATGACATTGGTGCTAATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC15360.1333589169450042No Hit
CTATTACTGCACCTCATACACAACCGGCAGCATGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC15330.133098450310346No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15280.1326643392525823No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15220.13214340598326588No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15110.13118836165618578No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14860.12901780636736734No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14630.1270208955016544No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14280.12398211809730861No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14130.12267978492401753No Hit
TCTCTGGGCTCCAGGCTGAGGACGCGGGTGACTATTACTGCACCTCATAC14010.1216379183853847No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14000.12155109617383196No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13690.1188596076156971No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACATTGGTGCTAATAACTATGTCTCCTGGTACCAACAACA13610.11816502992327521No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13440.11668905232687869No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13110.11382391934563836No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13050.11330298607632194No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12970.11260840838390003No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12650.10983009761421246No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12630.10965645319110698No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12160.10557580924812832No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12140.10540216482502285No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG11610.1008005876127278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA204.919558E-4102.35068570
ACGAACG200.00653632453.32477652
CTAACGC601.6002008E-533.6265683
CTAATGG1857.546987E-933.19481370
CCTAACG752.0342613E-631.3943392
TATCAAC54200.029.4787392
ATCAACG56600.028.2201773
GTATCAA59500.028.1604881
TCAACGC57300.027.9316844
CAACGCA58250.027.5338735
AACGCAG58600.027.4267966
TACGTAT1000.00816459927.29351470
AACGAAC1056.0454295E-727.01739151
CGGGTAT757.326157E-526.90826417
TCTATAC500.00876559726.9012553
TAACGCC757.3434996E-526.8989184
AATGGTA5600.026.8061370
GTGCTAG2650.026.662521
ATAGCGG708.945399E-426.38970863
CGCAGAG61200.026.0967868
Produced by FastQC (version 0.11.5)