FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702640
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC48910.28725978480477377No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG43460.25525066954846587No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGC39780.2336371752102617No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGCAGTGGTGAGGTACCACACCTGACAGAAATAGTCAC39660.23293238735140723No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG38570.22653056430014568No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC36960.2170746605271813No Hit
GGATTGGACTTAGGAATGTCCACTGGTTCCAGCAGAAGGCAGGCCAGGCCCCTGTCCTCGTCATCTACAGAGATG35450.20820607996992904No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34830.20456467603251421No Hit
CTTTACAGGTTCTGTGGCCTCCTATGCGGTGACGCAGCCACTTGCAGTGT33020.19393412582812572No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32560.1912324390358502No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA31960.18770849974157777No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC31930.18753230277686417No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT30950.18177653526288587No Hit
GTAATAAATGCCCGAATCGTCGCCTCGCAGGCTGTTCATTTGCAGAGAAA29340.17232063148992152No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28930.16991260630550203No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA28690.16850303058779306No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC27740.1629234600385284No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT27700.16268853075224357No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT27570.16192501057181788No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGCGGTGACG26590.15616924305783958No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC26520.15575811680684112No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG26290.15440727341070337No Hit
GTCCAATCCTGTCTCCTTCACAAGAAATCTTGACCGTTTGACCCAGGGCCACTGACACTGCAAGTGGCTGCGTCA25960.15246910679885356No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25950.15241037447728234No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25820.15164685429685665No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC25690.15088333411643096No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25240.14824037964572664No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25080.14730066250058732No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG25040.1470657332143025No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24820.14577362213973594No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC24770.14547996053187992No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT24540.14412911713574214No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC24290.14266080909646198No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23950.14066391016304092No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22960.13484941032749143No Hit
GTACCACACCTGACAGAAATAGTCACCCGCATCTCCGGCTTGGGCCTGGCTGATGGTCAGGGTGGCCGTGTTCCC22630.1329112437156416No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22230.13056195085279332No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21740.12768406709580418No Hit
CGGCCACCCTGACCATCAGCCAGGCCCAAGCCGGAGATGCGGGTGACTATTTCTGTCAGGTGTGGTACCTCACCA21410.12574590048395434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21290.12504111262509984No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21150.12421886012310296No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG21140.12416012780153173No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21130.12410139547996053No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20740.12181083493868347No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20550.12069492082883052No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG20340.11946154207583518No Hit
GTGGTGATGGTGCCACCACGGACTACGCAGCCTCCGTGAAGGGCCGCTTC20130.11822816332283982No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC19850.11658365831884604No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19380.1138232392049993No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCCCAGGGATCCCA19120.1122961988441479No Hit
CCTATGCGGTGACGCAGCCACTTGCAGTGTCAGTGGCCCTGGGTCAAACG18910.11106282009115256No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18610.10930085044401636No Hit
GGCCAGGCCCCTGTCCTCGTCATCTACAGAGATGCAAAGCGGCCCTCTGG18310.10753888079688015No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG18040.10595310811445754No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17910.10518958793403187No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGCAGTGGTGAGGTACCACACCTGACAGAAATAGTCACC17890.10507212329088943No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC17790.10448480007517738No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17630.10354508293003806No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17440.10242916882018513No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17430.10237043649861392No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17430.10237043649861392No Hit
GTGTAAAAGATGCGGGTGACTTACTGCGATCTGGCGGAGACTACTTTGAC17300.10160691631818822No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA17280.1014894516750458No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG17250.1013132547103322No Hit
GTGTGGTACCTCACCACTGCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC17170.10084339613776253No Hit
CTCCAGCCCCTTCCCTCGAGCCTGGCGAATCCAGTTCATGGCATAGTTGT17030.10002114363576564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA200.00761805851.3002136
CGATTGT400.00789231850.8410970
TTATACA3400.045.7516142
CTTATAC3400.045.7516141
GGTATCA10100.045.534971
TATACAC3650.042.6179433
TCGTATG3150.041.5759540
AGACTAA3750.041.51560625
CGTATGC3200.040.99012841
TCTCGTA2950.040.7058238
AATCTCG3400.039.22957236
CGAGACT4000.038.9014423
TAAGGCG4050.038.52871729
GACTAAG4050.038.456226
ATCTCGT3450.037.72463237
GTATCAA23400.037.5737881
GCCGTCT3250.037.50948746
ACTAAGG4200.037.10022427
ACGAGAC4300.036.96972322
GTGGTAT2400.036.6344451
Produced by FastQC (version 0.11.5)