FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702640
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC45900.2695813560118404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45460.2669971338627073No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG38560.22647183197857446No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC37120.21801437767232065No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGC37050.2176032514213222No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG37040.21754451909975098No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGCAGTGGTGAGGTACCACACCTGACAGAAATAGTCAC36070.21184748390734387No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34910.2050345346050839No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC34150.20057087816567212No Hit
GGATTGGACTTAGGAATGTCCACTGGTTCCAGCAGAAGGCAGGCCAGGCCCCTGTCCTCGTCATCTACAGAGATG33860.1988676408401071No Hit
CTTTACAGGTTCTGTGGCCTCCTATGCGGTGACGCAGCCACTTGCAGTGT31630.18577033312972796No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31150.18295118169431002No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28750.16885542451722033No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA28530.16756331344265377No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT28490.16732838415636894No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28300.166212470046516No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG27720.162805995395386No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC27310.1603979702109665No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGCGGTGACG27240.15998684395996804No Hit
GTAATAAATGCCCGAATCGTCGCCTCGCAGGCTGTTCATTTGCAGAGAAA26540.15587558144998356No Hit
GTCCAATCCTGTCTCCTTCACAAGAAATCTTGACCGTTTGACCCAGGGCCACTGACACTGCAAGTGGCTGCGTCA26470.1554644551989851No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25700.15094206643800215No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC25320.1487102382182963No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG25250.14829911196729784No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24970.14665460696330404No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG24450.14360052624160127No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG24160.14189728891603626No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23660.13896067283747593No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC23400.13743363247662455No Hit
GGGTGGCCGTGTTCCCCGAGTTGGAGCCAGAGAATCGCCCAGGGATCCCA23340.1370812385471973No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23330.1370225062256261No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22740.13355729925292487No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22460.13191279424893107No Hit
GTGGTGATGGTGCCACCACGGACTACGCAGCCTCCGTGAAGGGCCGCTTC22410.13161913264107505No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC22380.1314429356763614No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22220.1305032185312221No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC22030.12938730442136917No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22010.12926983977822676No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21980.12909364281351315No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG21150.12421886012310296No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT20950.1230442136916788No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20650.12128224404454259No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20500.1204012592209745No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT20490.12034252689940327No Hit
GTACCACACCTGACAGAAATAGTCACCCGCATCTCCGGCTTGGGCCTGGCTGATGGTCAGGGTGGCCGTGTTCCC20490.12034252689940327No Hit
CCTATGCGGTGACGCAGCCACTTGCAGTGTCAGTGGCCCTGGGTCAAACG20430.11999013296997603No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20210.11869802189540948No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19940.1171122492129869No Hit
GTGTGGTACCTCACCACTGCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19740.11593760278156276No Hit
CGGCCACCCTGACCATCAGCCAGGCCCAAGCCGGAGATGCGGGTGACTATTTCTGTCAGGTGTGGTACCTCACCA19710.11576140581684913No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19330.11352957759714327No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC19290.11329464831085843No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18730.11000563830287084No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18670.1096532443734436No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18140.10654043133016963No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC18050.10601184043602875No Hit
CTCCAGCCCCTTCCCTCGAGCCTGGCGAATCCAGTTCATGGCATAGTTGT18040.10595310811445754No Hit
GGCCAGGCCCCTGTCCTCGTCATCTACAGAGATGCAAAGCGGCCCTCTGG17950.10542451722031668No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17510.10284029507118357No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17510.10284029507118357No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17240.10125452238876097No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17120.1005497345299065No Hit
GTGTAAAAGATGCGGGTGACTTACTGCGATCTGGCGGAGACTACTTTGAC17030.10002114363576564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3400.038.736611
GGTATCA10000.037.8228231
GTATCAA23550.037.713841
TGGTATC3700.034.672912
TATCAAC27350.032.340922
TCAACGC27350.032.340924
CAACGCA27350.032.2212645
ATCAACG27350.032.2165343
AACGCAG27650.031.8791586
GAATACG1500.031.5384779
ACGCAGA28550.030.884197
AGAGTAC29000.030.07226411
CAGAGTA29450.029.83792310
ATACGTA1700.029.8254311
TACGTAC1600.029.57775512
AATACGT1600.029.57253810
CGCAGAG30000.029.3975018
GCAGAGT30350.029.3933569
GTGCTAG1750.028.9460411
ACGTACC1750.027.0425213
Produced by FastQC (version 0.11.5)