FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883840
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40020.45279688631426507No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39340.4451031860970312No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27640.3127262853005069No Hit
CACTTTACACTGAAAATCAGCAGCGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGTTTTACAAATT27600.31227371469949317No Hit
CCCCAGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG24940.2821777697320782No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24900.2817251991310644No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24740.2799149167270094No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22880.25887038377986965No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22150.2506109703113686No Hit
GTAATGGATACAACTACTTGGATTGGTACCTGCAGAAGCCCGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT21690.24540640839971037No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21540.24370926864590875No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21510.24336984069514844No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21070.2383915640839971No Hit
GTGTAAAGTGTGTGCCTGACCCACTGCCACTGAACCTGTCAGGGACCCCG20990.23748642288196958No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20940.2369207096307024No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20560.2326212889210717No Hit
GGCACACACTTTACACTGAAAATCAGCAGCGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGTTTTA20360.2303584359160029No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20010.22639844315713253No Hit
GTGTGTGCCTGACCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAG19260.21791274438812455No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19060.21564989138305574No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18750.2121424692251991No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18130.20512762490948588No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTACTTGGATTGGTACCTGCAGAAGCCCGGGCAGTCTC17740.20071506154960175No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17650.19969677769732078No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17270.1953973569876901No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17040.19279507603186097No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT15740.17808653149891382No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAAGCTTGGTCC15110.17095854453294715No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15060.17039283128167992No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14900.16858254887762492No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14890.16846940622737147No Hit
GTGCAGCCACAGTTCGTTTGATCTCAAGCTTGGTCCCCTGGCCAAAATTG14670.16598026792179582No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14440.1633779869659667No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14420.1631517016654598No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCAGGAGAGCCGGCCTCCATCTCCT14250.16122827661115136No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14000.15839971035481534No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA13660.1545528602461984No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13550.15330829109341057No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13200.1493482983345402No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13170.14900887038377986No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13000.1470854453294714No Hit
GCCCTATATTTCTGCGCGACCCGCTTCGGTGGTAACTCTGAGGAGCACTA12990.14697230267921796No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12790.14470944967414917No Hit
CTACTGGGGCCAGGGAACCCAGGTCACCGTCTCGTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT12710.14380430847212167No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC12580.14233345401882694No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCGGGCTTCTGCAGGTA12540.1418808834178132No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12460.14097574221578565No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGGTCAGGCACACACTTTACACTGAA12350.13973117306299782No Hit
CTCCTGTGTCGCCTCTGGATTCACCTTTAGCAGATATGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG12330.13950488776249095No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12250.13859974656046345No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11970.13543175235336713No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11820.13373461259956554No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11730.1327163287472846No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11730.1327163287472846No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11490.13000090514120202No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC11240.12717233888486604No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11240.12717233888486604No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11180.1264934829833454No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11050.1250226285300507No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11040.12490948587979725No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10850.1227597755249819No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10820.12242034757422157No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10710.12117577842143375No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10670.12072320782041998No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10630.12027063721940623No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10560.11947863866763216No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10560.11947863866763216No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10490.11868664011585807No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10440.11812092686459089No Hit
GCCTGAGACCCGAGGACACGGCCCTATATTTCTGCGCGACCCGCTTCGGT10370.1173289283128168No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10330.11687635771180305No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10210.11551864590876176No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10040.1135952208544533No Hit
GTTCAGTGGCAGTGGGTCAGGCACACACTTTACACTGAAAATCAGCAGCG10020.11336893555394642No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT9970.11280322230267921No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9930.11235065170166546No Hit
GGATACAACTACTTGGATTGGTACCTGCAGAAGCCCGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAAT9930.11235065170166546No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9820.11110608254887762No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9820.11110608254887762No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT9770.11054036929761042No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9750.11031408399710355No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9660.10929580014482258No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9640.10906951484431571No Hit
GAAATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTCCATTTGCAGATACA9570.10827751629254165No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9570.10827751629254165No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9470.10714608979000724No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9420.10658037653874006No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9420.10658037653874006No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9390.10624094858797972No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9350.10578837798696597No Hit
CTCCAGGGACAATTCGAAGAACATGCTGTATCTGCAAATGGACAGCCTGAGACCCGAGGACACGGCCCTATATTT9340.10567523533671253No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9280.1049963794351919No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9200.10409123823316438No Hit
AAATTGTACATGGGAATTTGTAAAACTTGCATGCAATAATAAACCCCAAC9180.1038649529326575No Hit
ATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTCCATTTGCAGATACAGCA9170.10375181028240406No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9120.10318609703113685No Hit
CTCCTGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC9120.10318609703113685No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9000.10182838522809559No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8990.10171524257784215No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8980.10160209992758869No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8970.10148895727733526No Hit
CTCCCTGCCCGTCACCCCAGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA8970.10148895727733526No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC8940.10114952932657494No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8930.1010363866763215No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8860.10024438812454743No Hit
GTACAATTTTGGCCAGGGGACCAAGCTTGAGATCAAACGAACTGTGGCTG8850.100131245474294No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8840.10001810282404054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTTA253.2871158E-668.2993515
CCGTCTT900.061.9048547
CGTATGC950.058.2368241
CCGTTAA309.6930835E-656.9161216
TCGTATG1000.055.27431540
CGTCTTC1050.053.1410148
ATGCCGT1050.052.8296744
GCCGTCT1100.050.5793946
TCTACAT1100.049.8640932
ATCTCGT1050.049.16634437
CATCTCG1050.049.12712536
TACATCT1050.049.04887834
CTACATC1050.048.99870733
GGTACCG352.4121975E-548.790776
CTTATAC1200.048.3978731
CACGAGA1150.047.52065321
ATCTCCG1150.047.5125910
GGTATCA6550.047.4633831
GTGGTAT1600.046.9744071
TATACAC1350.045.5380553
Produced by FastQC (version 0.11.5)