FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883840
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37750.42711350470673426No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34360.38875814627081823No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28810.3259639753801593No Hit
CACTTTACACTGAAAATCAGCAGCGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGTTTTACAAATT26450.2992623099203476No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25510.28862690079652426No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24760.28014120202751625No Hit
CCCCAGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG23080.2611332367849385No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22310.2524212527154236No Hit
GTGTGTGCCTGACCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAG22310.2524212527154236No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21060.23827842143374364No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG20830.23567614047791458No Hit
GTAATGGATACAACTACTTGGATTGGTACCTGCAGAAGCCCGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT20000.2262853005068791No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19970.22594587255611878No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19950.22571958725561186No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19830.2243618754525706No Hit
GTGTAAAGTGTGTGCCTGACCCACTGCCACTGAACCTGTCAGGGACCCCG19780.2237961622013034No Hit
GGCACACACTTTACACTGAAAATCAGCAGCGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGTTTTA19330.2187047429398986No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19210.21734703113685735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18640.2108979000724113No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18530.20965333091962343No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18380.20795619116582187No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTACTTGGATTGGTACCTGCAGAAGCCCGGGCAGTCTC18370.20784304851556842No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17600.19913106444605358No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17570.19879163649529327No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17100.1934739319333816No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT16180.18306480811006517No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16140.18261223750905142No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15490.17525796524257783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15490.17525796524257783No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15370.17390025343953658No Hit
GTGCAGCCACAGTTCGTTTGATCTCAAGCTTGGTCCCCTGGCCAAAATTG15170.17163740043446776No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14910.16869569152787833No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14840.16790369297610427No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAAGCTTGGTCC14330.16213341781317886No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13550.15330829109341057No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13470.15240314989138304No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13380.15138486603910212No Hit
GCCCTATATTTCTGCGCGACCCGCTTCGGTGGTAACTCTGAGGAGCACTA13370.15127172338884864No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCAGGAGAGCCGGCCTCCATCTCCT13100.1482168718320058No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13040.14753801593048516No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12720.14391745112237508No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12640.14301230992034758No Hit
CTCCTGTGTCGCCTCTGGATTCACCTTTAGCAGATATGCCATGAGCTGGGTCCGCCAGGCTCCAGGAAAGGGG12590.14244659666908038No Hit
CTACTGGGGCCAGGGAACCCAGGTCACCGTCTCGTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT12350.13973117306299782No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12280.13893917451122376No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC12200.13803403330919622No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11910.1347528964518465No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGGTCAGGCACACACTTTACACTGAA11880.13441346850108618No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11880.13441346850108618No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11840.13396089790007243No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11770.13316889934829834No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11670.13203747284576392No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11570.13090604634322955No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11520.13034033309196233No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11500.13011404779145547No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11480.12988776249094858No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA11370.12864319333816074No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11230.1270591962346126No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10870.12298606082548878No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10750.1216283490224475No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10610.12004435191889935No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC10570.1195917813178856No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCGGGCTTCTGCAGGTA10510.11891292541636495No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10390.11755521361332368No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT10270.11619750181028242No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10250.11597121650977552No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10210.11551864590876176No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10150.11483979000724114No Hit
CTCCCTGCCCGTCACCCCAGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA10140.1147266473569877No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9990.1130295076031861No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9910.11212436640115858No Hit
GCCTGAGACCCGAGGACACGGCCCTATATTTCTGCGCGACCCGCTTCGGT9900.11201122375090514No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9890.1118980811006517No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9730.11008779869659667No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9710.1098615133960898No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9660.10929580014482258No Hit
GTTCAGTGGCAGTGGGTCAGGCACACACTTTACACTGAAAATCAGCAGCG9660.10929580014482258No Hit
GGATACAACTACTTGGATTGGTACCTGCAGAAGCCCGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAAT9540.10793808834178131No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9510.10759866039102099No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9500.10748551774076756No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT9430.10669351918899349No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9330.1055620926864591No Hit
ATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTCCATTTGCAGATACAGCA9200.10409123823316438No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9140.10341238233164375No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9110.1030729543808834No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9030.1021678131788559No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9020.10205467052860247No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9010.10194152787834904No Hit
GAAATATAGGGCCGTGTCCTCGGGTCTCAGGCTGTCCATTTGCAGATACA9000.10182838522809559No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8980.10160209992758869No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGACGTGCAACTGCTGGAGT8960.10137581462708181No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8940.10114952932657494No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8920.10092324402606806No Hit
AAATTGTACATGGGAATTTGTAAAACTTGCATGCAATAATAAACCCCAAC8890.10058381607530775No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8840.10001810282404054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG100.009392894131.975970
ACATGCG200.007094485752.23029747
GGTATCA6500.044.623991
TGGTATC1800.043.590842
GTGGTAT2150.036.5049861
GTATCAA16750.036.059631
CGTTGAA601.3059547E-534.6380842
GTGTAAT501.9847597E-434.124231
GTAGTAC400.002747596534.1029853
ACGAACC652.085029E-532.34918252
ACGTAGG751.7896546E-631.91252327
TATCAAC19000.031.6008642
CGTAGGA652.459024E-531.5779628
TGTAATA652.5061543E-531.4903722
TACTCCG652.5139214E-531.4761165
GCTTGTA553.4723766E-431.0220241
GGACGAG450.00439165430.98888648
TAGCGGC702.9362149E-530.76851364
TGTATAT450.004817319630.40840528
AACCGTG450.004894036330.3103358
Produced by FastQC (version 0.11.5)