FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920381
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACAGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGACCAGTCACCAGTGATTA22780.24750619580369435No Hit
CCATAGTAGAGCAGGCAGTAATAATCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCC22490.24435532676141727No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC20160.21903972376657058No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19960.2168667106339657No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC18880.20513243971789943No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGAGCACCACCATAGTAGAGCAGGCAGTAATAATCAG17740.19274626486205168No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16840.1829677057653298No Hit
CTACTATGGTGGTGCTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC16390.17807842621696884No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16080.1747102558614313No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15800.1716680374757845No Hit
GATTATTACTGCCTGCTCTACTATGGTGGTGCTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAG15410.167430661867205No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA15370.16699605924068403No Hit
GTAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGCTCCAGGTAGGCTGTGCTCGTGGATGTGTCTGTGGT15310.16634415530090257No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG14960.16254138231884405No Hit
GCACTGGACCAGTCACCAGTGATTACTATCCAAACTGGTTCCAGCGGAAA14890.16178082772243235No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC14290.15526178832461773No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14270.15504448701135726No Hit
GTCCAGGAGTGTTTGTAACTTGTACCATAAATCAGTGCCCTGGGTGCCTG13860.15058981008951727No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13850.15048115943288703No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13670.14852544761354267No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13500.1466783864508285No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT12960.14081125099279537No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12860.13972474442649294No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12740.13842093654693002No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG12710.1380949845770393No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12540.13624792341432518No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12500.1358133207878042No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12290.13353165699856906No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12100.13146729452259445No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11970.13005483598640127No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11700.1271212682573847No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11540.1253828577513008No Hit
GTGGTGACTCAGGAGCCCTCACAGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGA11490.12483960446814961No Hit
GATTTATGGTACAAGTTACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC10940.11886381835348621No Hit
GTGTATTACTGTGCGAGAAGGGCTGGATCTTGTAGTGGTGGTTTCTGCTACCATGACTTCTGGGGCCAGGGAACC10790.11723405850403257No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10610.11527834668468818No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10380.11277938158219258No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG9830.10680359546752921No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9400.10213161723242874No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9240.10039320672634484No Hit
GCCTGAGGACGAGGCTGATTATTACTGCCTGCTCTACTATGGTGGTGCTCCGGTGTTCGGCGGAGGGACCAAG9220.10017590541308435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG200.00785154350.9080965
GGTATCA9650.038.6950451
CGTATGC1009.094947E-1238.0269341
GTATCAA25100.037.191951
TCGTATG952.1827873E-1036.32506640
TAGTACC400.002813465433.9387324
AACGCTA651.7208904E-533.2697961
CGAGACG1350.032.67996223
GTGGTAT2650.032.024631
ATCAACG29350.031.6838553
TATCAAC29550.031.4694142
TCAACGC29650.031.3632774
CAACGCA30000.030.8842455
CTTAACG703.031798E-530.62473758
GAGTTTA3900.030.4644551
AACGCAG30650.030.2292776
TACGTTA703.5545498E-529.9195447
ATTACGT703.6215308E-529.83782645
GGCTAAG900.00566853929.4102170
CGCAGAG32600.028.517448
Produced by FastQC (version 0.11.5)