FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920381
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACAGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGACCAGTCACCAGTGATTA21760.2364238288274095No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20790.2258847151342759No Hit
CCATAGTAGAGCAGGCAGTAATAATCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTTTGCCC19430.2111082258325628No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGC19090.20741410350713455No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC18780.20404593315159703No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGAGCACCACCATAGTAGAGCAGGCAGTAATAATCAG17220.18709643071727905No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17120.1860099241509766No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16710.18155524722913663No Hit
CTACTATGGTGGTGCTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC16420.17840437818685959No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA16390.17807842621696884No Hit
GATTATTACTGCCTGCTCTACTATGGTGGTGCTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAG16240.1764486663675152No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15090.1639538408550372No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14790.16069432115612992No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14690.15960781458982748No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14670.15939051327656698No Hit
GTAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGCTCCAGGTAGGCTGTGCTCGTGGATGTGTCTGTGGT14570.15830400671026454No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14430.15678289751744115No Hit
GTCCAGGAGTGTTTGTAACTTGTACCATAAATCAGTGCCCTGGGTGCCTG14380.15623964423428993No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14340.15580504160776895No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG14230.15460988438483628No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13930.151350364685929No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT13630.14809084498702169No Hit
GTGGTGACTCAGGAGCCCTCACAGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGA13360.14515727725800512No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC12640.1373344299806276No Hit
GCACTGGACCAGTCACCAGTGATTACTATCCAAACTGGTTCCAGCGGAAA12520.13603062210106467No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG12400.13472681422150176No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12250.1330970543720481No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11980.13016348664303154No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11710.12722991891401494No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11150.12114548214272132No Hit
GATTTATGGTACAAGTTACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC10920.11864651704022572No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10750.11679945587751159No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10690.11614755193773013No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10520.114300490775016No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10380.11277938158219258No Hit
GTGTATTACTGTGCGAGAAGGGCTGGATCTTGTAGTGGTGGTTTCTGCTACCATGACTTCTGGGGCCAGGGAACC10020.10886795794350383No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9740.10582573955785703No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9740.10582573955785703No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG9670.10506518496144532No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9550.10376137708188239No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9490.10310947314210092No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9480.1030008224854707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3750.040.6835561
GGTATCA11650.037.249611
TGGTATC4150.035.9374922
GTATCAA28950.034.664171
GTATAGA502.0621397E-433.9029661
ATCCGTA400.002840883633.87165512
ATGGCAA2254.3655746E-1133.1748270
TATCAAC30950.032.088562
ATCAACG32600.030.6523933
TCAACGC32850.030.5205784
CAACGCA33150.030.450385
AACGCAG33250.030.35886
CGCAGAG34800.028.6172628
ACGCAGA35100.028.5656817
AGAGTAC35100.028.17965911
CAGAGTA36200.027.32336810
ATAGCGG1355.6188583E-926.82584263
ACCCGCT700.001005012725.86777963
GCAGAGT39700.024.9145099
ACGAACC1301.4964644E-724.40970852
Produced by FastQC (version 0.11.5)