FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007359
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTACACAGCTCCAATCATAAGAACTACTTAACTTGGTACCAGCAGAA81760.8116272351763373No Hit
GTGTAAAACAGTCTGGTTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCAAGGGACTCTGGAGA58160.5773512719894297No Hit
CTACTAAGAGACTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAAT53680.532878546774288No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51300.509252411503744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50610.5024028176648047No Hit
ACTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGTTAAGTAGTTCTTATGATTGGAGCTGTGTAAAACAGT47460.47113293274790813No Hit
CTCTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAA46700.46358845257748227No Hit
CTCCTAAGTTGCTCATTTACTGGGCGTCTAACCGGCAATCCGGGGTCCCT44180.4385725446439651No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40390.40094941326776257No Hit
GTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA40300.4000559879844226No Hit
GTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCGCTCTCAC36200.35935550285449375No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36140.3587598859989338No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35580.35320079534704113No Hit
GGCCAAAACTGTACGGACTACTAAGAGACTGCTGACAGAAATAAACTGCC34710.34456435094142207No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32900.3265965757986974No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32240.32004479038753814No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29670.2945325350743876No Hit
GTCAGGGACCCCGGATTGCCGGTTAGACGCCCAGTAAATGAGCAACTTAGGAGGCTGTCGTGGTTTCTGCTGGTA29420.2920507981762212No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28660.28450631800579534No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27960.27755745469092946No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27030.2683253934297505No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26510.26316338068156436No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26040.2584977153130115No Hit
ACTAAGAGACTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAAT25900.25710794265003833No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25240.2505561572388791No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25180.24996054038331916No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24890.24708172558144614No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTG24540.2436072939240132No Hit
CTTCAGTGGCAGCGGGTCTGGGACAGATTTCGCTCTCACAATCAGCAGCC24420.24241606021289333No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24310.2413240959777001No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23990.2381474727480471No Hit
GTCTCTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT23530.23358107685542095No Hit
GTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCGGATTGCCGGTT23430.2325883820961544No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22920.22752563882389495No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC20920.20767174363856383No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19930.19784406552182487No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGACTGTTTTACACAGCTCCAATCATAAGAACTA19600.19456817281624525No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19480.19337693910512538No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19360.19218570539400553No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18290.18156387146985334No Hit
GCTCCAATCATAAGAACTACTTAACTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGTTGCTCATTTACTGGG18040.17908213457168695No Hit
CCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAAC17900.1776923619087138No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17880.17749382295686045No Hit
TCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACC17640.17511135553462073No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17250.17123984597348116No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16100.15982385624191572No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16020.1590297004345025No Hit
GTCCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA15980.15863262253079588No Hit
CTACTTAACTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGTTGCTCATTTACTGGGCGTCTAACCGGCAATC15850.15734211934374934No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15660.15545599930114287No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15490.15376841821038975No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15340.1522793760714899No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15300.1518822981677833No Hit
CCAGAGTCCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGACTGTTTTACACAG15180.15069106445666341No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAATCTGTCCCAGACCCGCTGCCA15180.15069106445666341No Hit
ACTTAACTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGTTGCTCATT15120.15009544760110347No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15060.14949983074554354No Hit
GTCTGGGACAGATTTCGCTCTCACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAGT14980.1487056749381303No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14970.14860640546220363No Hit
CTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACG14850.1474151717510838No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14770.14662101594367052No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14700.14592612961218393No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14700.14592612961218393No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14630.14523124328069736No Hit
GGTCTGGGACAGATTTCGCTCTCACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAGT14580.14473489590106406No Hit
GTTTATTTCTGTCAGCAGTCTCTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA14390.1428487758584576No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14340.14235242847882434No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14260.14155827267141108No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14140.1403670389602912No Hit
CCACTGAAGCGGTCAGGGACCCCGGATTGCCGGTTAGACGCCCAGTAAAT14120.14016850000843792No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14060.13957288315287797No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14010.1390765357732447No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13990.1388779968213914No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13650.13550283463988508No Hit
GTAAATGAGCAACTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGTTA13630.13530429568803176No Hit
AAACTGTACGGACTACTAAGAGACTGCTGACAGAAATAAACTGCCACATC13610.13510575673617847No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13580.1348079483083985No Hit
GTTAAGTAGTTCTTATGATTGGAGCTGTGTAAAACAGTCTGGTTGGACTT13400.1330210977417187No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13370.13272328931393873No Hit
CTACGGGGACATCGTGGTGACCCAGTCTCCAGAGTCCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13180.13083716927133227No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13160.13063863031947895No Hit
GTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTG13160.13063863031947895No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13060.1296459355602124No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12940.12845470184909252No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12900.12805762394538592No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12900.12805762394538592No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12900.12805762394538592No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12780.12686639023426605No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGGTGACCCAGTCTCCAGAGTCCCTTGCTGTGTCTCTGGGCGAGAGGG12770.12676712075833937No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12590.12498027019165958No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAGTCTCTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAA12560.1246824617638796No Hit
CATTTACTGGGCGTCTAACCGGCAATCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCG12500.12408684490831969No Hit
GACTACTAAGAGACTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAAT12440.12349122805275974No Hit
CCCAGAGACACAGCAAGGGACTCTGGAGACTGGGTCACCACGATGTCCCC12120.12031460482310675No Hit
CATAAGAACTACTTAACTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGTTGCTCATTTACTGGGCGTCTAAC12110.1202153353471801No Hit
CTCACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCA11930.11842848478050029No Hit
AACCACGACAGCCTCCTAAGTTGCTCATTTACTGGGCGTCTAACCGGCAA11760.11674090368974716No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11490.11406062783972744No Hit
GTTTGGTCCCCTGGCCAAAACTGTACGGACTACTAAGAGACTGCTGACAGAAATAAACTGCCACATCTTCAGC11430.11346501098416752No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11350.11267085517675426No Hit
CACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGC11290.11207523832119433No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11250.11167816041748771No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11210.11128108251378108No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA11210.11128108251378108No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGGTGA11160.1107847351341478No Hit
GATTTCGCTCTCACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAGTCTCTTAGTAGT11080.10999057932673456No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGC11070.1098913098508079No Hit
GTCTCCAGAGTCCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGACTGTTTTACA11050.1096927708989546No Hit
GGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10960.10879934561561469No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC10820.10740957295264152No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG10720.10641687819337496No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT10610.10532491395818175No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10320.10244609915630873No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10250.10175121282482215No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10240.10165194334889548No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT10220.10145340439704216No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT10190.1011555959692622No Hit
GCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTGTACGG10150.10075851806555557No Hit
ACCATCAACTGCAAGTCCAACCAGACTGTTTTACACAGCTCCAATCATAA10130.10055997911370226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)