FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007359
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTACACAGCTCCAATCATAAGAACTACTTAACTTGGTACCAGCAGAA80790.8019980960114517No Hit
CTACTAAGAGACTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAAT52330.5194771675241895No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50770.5039911292796312No Hit
GTGTAAAACAGTCTGGTTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCAAGGGACTCTGGAGA49140.48781020470358627No Hit
CTCTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAA45400.45068342070701706No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44590.442642593156958No Hit
ACTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGTTAAGTAGTTCTTATGATTGGAGCTGTGTAAAACAGT42880.42566751277349985No Hit
CTCCTAAGTTGCTCATTTACTGGGCGTCTAACCGGCAATCCGGGGTCCCT42200.4189171884104872No Hit
GTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA41470.4116705166678413No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38720.38437141078801107No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38570.3828823686491112No Hit
GGCCAAAACTGTACGGACTACTAAGAGACTGCTGACAGAAATAAACTGCC34870.3461526625562486No Hit
GTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCGCTCTCAC32960.3271921926542573No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32060.3182579398208583No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31450.31220250178933234No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30370.3014813983892535No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29490.29274568450770777No Hit
GTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCGGATTGCCGGTT28080.2787486884020493No Hit
GTCAGGGACCCCGGATTGCCGGTTAGACGCCCAGTAAATGAGCAACTTAGGAGGCTGTCGTGGTTTCTGCTGGTA26910.2671341597186306No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26750.2655458481038041No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26610.2641560754408309No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26550.263560458585271No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25460.2527400857092655No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTG25180.24996054038331916No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25120.2493649235277592No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24710.24529487501476635No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24430.24251532968881995No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24380.2420189823091867No Hit
ACTAAGAGACTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAAT24320.24142336545362678No Hit
GTCTCTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT23880.2370555085128539No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23870.23695623903692725No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23520.2334818073794943No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23150.22980883677020802No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21510.21352864271823652No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20800.20648050992744393No Hit
CTTCAGTGGCAGCGGGTCTGGGACAGATTTCGCTCTCACAATCAGCAGCC20730.20578562359595734No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19910.19764552656997159No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19640.19496525071995188No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGACTGTTTTACACAGCTCCAATCATAAGAACTA19540.19397255596068533No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC18590.18454195574765303No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18000.17868505666798035No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17690.175607702914254No Hit
TCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACC17420.1729274270642343No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17400.172728888112381No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17370.172431079684601No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16990.1686588395993881No Hit
GCTCCAATCATAAGAACTACTTAACTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGTTGCTCATTTACTGGG16780.16657418060492835No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16270.1615114373326689No Hit
GTCTGGGACAGATTTCGCTCTCACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAGT16140.16022093414562236No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15950.1583348141030159No Hit
CCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAAC15930.1581362751511626No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15900.15783846672338264No Hit
GGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAGTCTCTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAA15510.15396695716224307No Hit
GGTCTGGGACAGATTTCGCTCTCACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAGT15500.1538676876863164No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15390.1527757234511232No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15180.15069106445666341No Hit
GTCCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA15100.14989690864925018No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14960.148507135986277No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14850.1474151717510838No Hit
CTACTTAACTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGTTGCTCATTTACTGGGCGTCTAACCGGCAATC14850.1474151717510838No Hit
GAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAATCTGTCCCAGACCCGCTGCCA14700.14592612961218393No Hit
CCAGAGTCCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGACTGTTTTACACAG14650.14542978223255065No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14630.14523124328069736No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14550.1444370874732841No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGGTGACCCAGTCTCCAGAGTCCCTTGCTGTGTCTCTGGGCGAGAGGG14240.1413597337195578No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14100.13996996105658457No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13920.13818311048990478No Hit
GTTTATTTCTGTCAGCAGTCTCTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAA13860.13758749363434486No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13760.1365947988750783No Hit
CTTAGTAGTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACG13740.136396259923225No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13680.13580064306766504No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13640.13540356516395843No Hit
CTACGGGGACATCGTGGTGACCCAGTCTCCAGAGTCCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG13140.13044009136762563No Hit
AAACTGTACGGACTACTAAGAGACTGCTGACAGAAATAAACTGCCACATC12960.12865324080094584No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12850.12756127656575264No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12730.12637004285463274No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12700.12607223442685278No Hit
GACTACTAAGAGACTGCTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATTGTGAGAGCGAAAT12630.12537734809536621No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12620.12527807861943954No Hit
ACTTAACTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGTTGCTCATT12600.12507953966758623No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12380.12289561119719981No Hit
GTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTG12350.12259780276941984No Hit
GTAAATGAGCAACTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGTTA12270.1218036469620066No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12230.12140656905829997No Hit
CCACTGAAGCGGTCAGGGACCCCGGATTGCCGGTTAGACGCCCAGTAAAT12120.12031460482310675No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12120.12031460482310675No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12090.1200167963953268No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC11830.11743579002123374No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGGTGA11760.11674090368974716No Hit
GTTAAGTAGTTCTTATGATTGGAGCTGTGTAAAACAGTCTGGTTGGACTT11630.11545040050270064No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11560.11475551417121403No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11540.11455697521936073No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA11490.11406062783972744No Hit
CTCACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCA11410.11326647203231421No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT11390.11306793308046088No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11310.11227377727304763No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11240.11157889094156105No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11220.11138035198970775No Hit
GTTTGGTCCCCTGGCCAAAACTGTACGGACTACTAAGAGACTGCTGACAGAAATAAACTGCCACATCTTCAGC11020.10939496247117461No Hit
CATTTACTGGGCGTCTAACCGGCAATCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCG10950.10870007613968803No Hit
GTCTCCAGAGTCCCTTGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAACCAGACTGTTTTACA10940.10860080666376139No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10920.10840226771190807No Hit
GATTGTGAGAGCGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCGGATTGCCGGTTAGACGC10850.10770738138042148No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10770.10691322557300822No Hit
GGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10680.10601980028966834No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG10680.10601980028966834No Hit
CACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGC10650.10572199186188837No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10530.1045307581507685No Hit
GATTTCGCTCTCACAATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAGCAGTCTCTTAGTAGT10520.10443148867484184No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10500.10423294972298854No Hit
AACCACGACAGCCTCCTAAGTTGCTCATTTACTGGGCGTCTAACCGGCAA10480.1040344107711352No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10440.10363733286742859No Hit
CATAAGAACTACTTAACTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGTTGCTCATTTACTGGGCGTCTAAC10400.10324025496372197No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10390.10314098548779532No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10350.10274390758408869No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGC10310.10234682968038207No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10160.10085778754148222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGAGT200.00511454256.7344963
AAAACCG1055.853144E-849.34972470
GCCCGTT305.0665723E-448.09565454
TGGTATC2400.045.7307132
GGTATCA6100.037.0807951
AATGGTA2001.7826096E-1037.0122970
GTATCAA14500.035.78711
AACGGAA551.772586E-434.7871265
TCGTAGC400.003107014633.2586982
CTACGGA4050.031.39995469
TATCAAC16650.031.3610572
CGGGATA1400.030.91684217
AGCTAAG450.005103020530.05260535
GGGATAC1451.8189894E-1229.85222618
ATCAACG17500.029.8363253
CGAACTA450.00537161329.73896429
GAGCATT450.00550535429.58972715
TCAACGC17750.029.228734
GACAGGT1152.5156623E-928.9249151
CAACGCA18000.028.8227755
Produced by FastQC (version 0.11.5)