FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294863
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT19960.6769245378362154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.41239490882206314No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9430.3198095386671098No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6880.2333286984124831No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6750.2289198712622472No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG6540.2217979197118662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.21840651421168475No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6000.20348433001088642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.19229269186028766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.1902578485601788No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5520.1872055836100155No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG5350.18144019425970706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.16821371280899944No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC4790.16244832345869098No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT4330.14684785815785636No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG4240.14379559320769308No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGG4110.13938676605745717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.13362137670714874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.13260395505709432No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3770.1278559873568403No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC3650.12378630075662257No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG3420.11598606810620526No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC3400.11530778700616898No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT3330.11293380315604196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.11293380315604196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.10988153820587866No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACC3080.10445528940558836No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.1024204461054795No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC3010.10208130555546134No Hit
AGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTTACAAAATGGATTGTTTCAAATGGCAATTT2970.10072474335538877No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.10038560280537064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2850.0102.39609570
CGTCGCT156.520837E-495.32329668
CTAGGCG150.00111352183.3159360
GCGTGAA251.2365916E-463.9668956
GTCCGCG200.003938806260.5892457
ACCTAAC1500.056.0537871
CCGGCTT200.005652501755.30684747
ACGTTAG402.6740963E-854.496731
CGCGTAT200.007601082351.31733739
TCTGGTC250.0076411251.24569362
CCTAACG1650.050.9320642
CTAACGC1650.050.9320643
CGAATGT200.00897321149.20777534
AAGGGGG4200.048.50160669
TAACGCC1750.048.0216564
CTCTTAT651.0913936E-1147.909221
CTTGAAA3900.047.6428257
GGGATTT655.5046106E-447.2597470
AAAGGGG3950.047.05833468
ACGCCAT1800.046.687726
Produced by FastQC (version 0.11.5)