FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052142
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTGGTCGTGTCTGATGACACCGACCGGCCCTCAGGGATCCCTGAGCG33090.31450127454278987No Hit
CCTATGTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACG31890.30309596993561705No Hit
CTATATTACTGTGCGAGAAGTATAATTGTCCCTGATGCTTTTGATGTTTGGGGCCAAGGGACAATGGTCTCCGT30390.28883933917665106No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30140.2864632340501567No Hit
GTAATATAGAGCCGTGTCTTCAGGTGTCAAGTTGTGCATGTGCAGAAAGA29400.2794299628757335No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29060.27619845990370123No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24360.23152768352560776No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22100.210047693182099No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21930.20843194169608287No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21660.20586574815946895No Hit
CCCCTGTGTTGGTCGTGTCTGATGACACCGACCGGCCCTCAGGGATCCCT20830.1979770791395078No Hit
ATATTACTGTGCGAGAAGTATAATTGTCCCTGATGCTTTTGATGTTTGGGGCCAAGGGACAATGGTCTCCGTCT20620.19598115083325254No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTTCTGACT20040.1904685869397857No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19770.1879023934031718No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19710.18733212817281317No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG19510.1854312440716177No Hit
CCCCAGGAGAGACGGCCAGACTTACCTGTGGGGGAGACAACATTGGAGAT19270.18315018315018314No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18630.17706735402635768No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18620.17697230982129789No Hit
CTCCAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGGAAAGCAAAGTTTC18590.17668717720611857No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18420.17507142572010242No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18150.17250523218348854No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17970.1707944364924126No Hit
TGGCCACCCTGACCGTCAGTGGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCACGTGTGGCATACTTCTA17940.1705093038772333No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17780.16898859659627694No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17630.16756293352038035No Hit
GGCTCTGTGACCTCCTATGTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAGACTTAC17260.16404629793316872No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA17220.16366612111292964No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTTCTGACTCAGCCAC17000.1615751486016146No Hit
GCCCTCAGGGATCCCTGAGCGATTCCTTGGCTCCAACTCTGGGAACATGG16930.1609098391661962No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16690.15862877824476165No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16690.15862877824476165No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACAGCCTCATCT16650.15824860142452254No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCCACTGACGGTCAGGGT16650.15824860142452254No Hit
CAGTAATATAGAGCCGTGTCTTCAGGTGTCAAGTTGTGCATGTGCAGAAA16450.15634771732332708No Hit
CTATTACTGTCACGTGTGGCATACTTCTACTAATCAGGGGGTAATCGGCG16320.15511214265755002No Hit
GTATAATTGTCCCTGATGCTTTTGATGTTTGGGGCCAAGGGACAATGGTC16230.15425674481201207No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16140.1534013469664741No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16120.15321125855635456No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16110.15311621435129477No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15840.1505500208146809No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15830.15045497660962115No Hit
GCACAGGCTCTGTGACCTCCTATGTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAGA15610.1483640040983061No Hit
CGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACAGCC15610.1483640040983061No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTTATTTTCAGAAACTTTGCT15430.1466532084072302No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15360.1459878989718118No Hit
GACTTACCTGTGGGGGAGACAACATTGGAGATGAGGCTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCC15260.14503745692121406No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTTCTGACTCAGC15250.14494241271615427No Hit
GGCCTGGAGTGGCTGGCCCTTATTTCATTTGATGGAAGTATCATCCATTA14850.14114064451376335No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14800.14066542348846447No Hit
ACATTGGAGATGAGGCTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTGGTCGTGTCTGATGACA14800.14066542348846447No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCCACTGACGGTCAGGGTGG14790.1405703792834047No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA14630.13904967200244833No Hit
GGGTGGCCATGTTCCCAGAGTTGGAGCCAAGGAATCGCTCAGGGATCCCTGAGGGCCGGTCGGTGTCATCAGACA14420.1370537436961931No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14220.13515285959499762No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14190.1348677269798183No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14130.13429746174945967No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13900.1321114450330849No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13580.12907003047117213No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC13210.12555339488396053No Hit
GTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAG13120.12469799703842258No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13010.12365251078276507No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAATTGGGTTTTC12890.12251198032204777No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGG12570.11947056576013504No Hit
GTTAAGAGGTATCGAATGTCAGATGCAATTGAGACAGTCTGGCGGAGGCG12270.11661923960834183No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12260.11652419540328207No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG12200.11595393017292342No Hit
CCCTGACCGTCAGTGGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGT12060.11462331130208661No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12060.11462331130208661No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12010.11414809027678774No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT11980.11386295766160841No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11550.10977605684403817No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11480.10911074740861974No Hit
GATTAGTAGAAGTATGCCACACGTGACAGTAATAGTCGGCCTCATCCCCG11300.10739995171754382No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11290.10730490751248406No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11260.10701977489730474No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11220.10663959807706565No Hit
GACTATTACTGTCACGTGTGGCATACTTCTACTAATCAGGGGGTAATCGGCGGGGGGACCAAGCTGACCGTCCTA11160.10606933284670701No Hit
GTGTCATCAGACACGACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTA11110.10559411182140814No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11100.10549906761634838No Hit
CCTGAGGGCCGGTCGGTGTCATCAGACACGACCAACACAGGGGCCTGGCC11080.10530897920622882No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11080.10530897920622882No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11060.10511889079610928No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11060.10511889079610928No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10900.10359818351515289No Hit
CAATTATACTTCTCGCACAGTAATATAGAGCCGTGTCTTCAGGTGTCAAGTTGTGCATGTGCAGAAAGAGTGTG10900.10359818351515289No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10810.10274278566961494No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG10790.1025526972594954No Hit
GGCCAGGCCCCTGTGTTGGTCGTGTCTGATGACACCGACCGGCCCTCAGG10780.10245765305443562No Hit
CTCCTGTGCAGCCTCTGGATTTATTTTCAGAAACTTTGCTTTCCACTGGGTCCGCCAGGCTCCAGGCAAGGGC10750.1021725204392563No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA10650.10122207838865857No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10620.10093694577347925No Hit
CCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAGACTTACCTGTGGGGGAGACAACATTGGAGATGAGGCTGTC10600.10074685736335971No Hit
TGTCTGATGACACCGACCGGCCCTCAGGGATCCCTGAGCGATTCCTTGGCTCCAACTCTGGGAACATGGCCACC10530.10008154792794127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT250.001236282381.1661670
ACCGGGT253.4332097E-667.80244418
GTCCGAG301.011725E-556.507411
ATCGCGT252.645366E-454.8652837
GGTATCA6050.048.1947481
TATACAC1800.047.087273
AATGGTA4750.045.56696770
TTATACA1800.043.3202862
ACACATC2000.042.3785446
CTTATAC1850.042.1514741
TCCGAGC2050.041.33902413
TCTCCGA2100.040.35284411
TACACAT2200.038.5259485
CACGAGA2150.037.85582421
CCCACGA2150.037.84502419
CGTATGC557.35478E-637.66530641
GTATCAA15600.037.1644861
ATCTCCG2300.036.843910
TCGTATG754.045978E-836.7731140
ATACACA2500.036.615064
Produced by FastQC (version 0.11.5)